Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06500 and PGA1_c06510 are separated by 163 nucleotides PGA1_c06510 and PGA1_c06520 are separated by 135 nucleotides PGA1_c06520 and PGA1_c06530 are separated by 148 nucleotides
PGA1_c06500: PGA1_c06500 - glycyl-tRNA synthetase beta subunit, at 665,103 to 667,367
_c06500
PGA1_c06510: PGA1_c06510 - pyruvate, phosphate dikinase PpdK, at 667,531 to 670,131
_c06510
PGA1_c06520: PGA1_c06520 - cell wall hydrolase-like protein, at 670,267 to 670,950
_c06520
PGA1_c06530: PGA1_c06530 - dihydroneopterin aldolase-like protein, at 671,099 to 672,019
_c06530
Position (kb)
667
668
669
670
671 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 667.407 kb on - strand at 667.490 kb on + strand at 667.497 kb on + strand at 667.505 kb on - strand at 667.638 kb on + strand at 667.688 kb on + strand at 667.688 kb on + strand at 667.696 kb on - strand at 667.718 kb on + strand at 667.745 kb on - strand at 667.782 kb on + strand at 667.782 kb on + strand at 667.790 kb on - strand at 667.790 kb on - strand at 667.795 kb on - strand, within PGA1_c06510 at 667.831 kb on - strand, within PGA1_c06510 at 667.865 kb on + strand, within PGA1_c06510 at 667.865 kb on + strand, within PGA1_c06510 at 667.865 kb on + strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.873 kb on - strand, within PGA1_c06510 at 667.881 kb on - strand, within PGA1_c06510 at 667.919 kb on + strand, within PGA1_c06510 at 667.924 kb on + strand, within PGA1_c06510 at 667.927 kb on - strand, within PGA1_c06510 at 667.929 kb on + strand, within PGA1_c06510 at 667.929 kb on + strand, within PGA1_c06510 at 667.931 kb on + strand, within PGA1_c06510 at 667.937 kb on - strand, within PGA1_c06510 at 667.937 kb on - strand, within PGA1_c06510 at 667.937 kb on - strand, within PGA1_c06510 at 667.937 kb on - strand, within PGA1_c06510 at 667.956 kb on + strand, within PGA1_c06510 at 668.019 kb on - strand, within PGA1_c06510 at 668.036 kb on - strand, within PGA1_c06510 at 668.136 kb on + strand, within PGA1_c06510 at 668.144 kb on - strand, within PGA1_c06510 at 668.144 kb on - strand, within PGA1_c06510 at 668.203 kb on - strand, within PGA1_c06510 at 668.374 kb on + strand, within PGA1_c06510 at 668.422 kb on + strand, within PGA1_c06510 at 668.432 kb on + strand, within PGA1_c06510 at 668.470 kb on + strand, within PGA1_c06510 at 668.470 kb on + strand, within PGA1_c06510 at 668.478 kb on - strand, within PGA1_c06510 at 668.596 kb on - strand, within PGA1_c06510 at 668.669 kb on + strand, within PGA1_c06510 at 668.669 kb on + strand, within PGA1_c06510 at 668.669 kb on + strand, within PGA1_c06510 at 668.677 kb on - strand, within PGA1_c06510 at 668.713 kb on + strand, within PGA1_c06510 at 668.718 kb on + strand, within PGA1_c06510 at 668.723 kb on + strand, within PGA1_c06510 at 668.726 kb on - strand, within PGA1_c06510 at 668.787 kb on - strand, within PGA1_c06510 at 668.837 kb on + strand, within PGA1_c06510 at 668.852 kb on + strand, within PGA1_c06510 at 668.852 kb on + strand, within PGA1_c06510 at 668.852 kb on + strand, within PGA1_c06510 at 668.852 kb on + strand, within PGA1_c06510 at 668.876 kb on - strand, within PGA1_c06510 at 668.943 kb on + strand, within PGA1_c06510 at 669.046 kb on + strand, within PGA1_c06510 at 669.054 kb on - strand, within PGA1_c06510 at 669.119 kb on - strand, within PGA1_c06510 at 669.189 kb on + strand, within PGA1_c06510 at 669.253 kb on - strand, within PGA1_c06510 at 669.328 kb on - strand, within PGA1_c06510 at 669.370 kb on - strand, within PGA1_c06510 at 669.437 kb on - strand, within PGA1_c06510 at 669.446 kb on - strand, within PGA1_c06510 at 669.548 kb on + strand, within PGA1_c06510 at 669.548 kb on + strand, within PGA1_c06510 at 669.667 kb on + strand, within PGA1_c06510 at 669.787 kb on - strand, within PGA1_c06510 at 669.802 kb on - strand, within PGA1_c06510 at 669.852 kb on + strand, within PGA1_c06510 at 669.895 kb on - strand at 670.184 kb on - strand at 670.197 kb on + strand at 670.198 kb on - strand at 670.267 kb on - strand at 670.389 kb on + strand, within PGA1_c06520 at 670.389 kb on + strand, within PGA1_c06520 at 670.389 kb on + strand, within PGA1_c06520 at 670.389 kb on + strand, within PGA1_c06520 at 670.397 kb on - strand, within PGA1_c06520 at 670.397 kb on - strand, within PGA1_c06520 at 670.397 kb on - strand, within PGA1_c06520 at 670.397 kb on - strand, within PGA1_c06520 at 670.473 kb on + strand, within PGA1_c06520 at 670.473 kb on - strand, within PGA1_c06520 at 670.481 kb on - strand, within PGA1_c06520 at 670.509 kb on + strand, within PGA1_c06520 at 670.579 kb on - strand, within PGA1_c06520 at 670.722 kb on - strand, within PGA1_c06520 at 670.906 kb on - strand at 670.906 kb on - strand at 670.926 kb on + strand at 670.944 kb on - strand at 671.011 kb on + strand at 671.055 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 667,407 - -1.2 667,490 + -0.5 667,497 + +0.7 667,505 - +0.1 667,638 + +0.2 667,688 + -2.1 667,688 + -1.3 667,696 - -3.3 667,718 + -0.3 667,745 - -1.3 667,782 + -0.6 667,782 + -0.3 667,790 - -1.2 667,790 - -1.7 667,795 - PGA1_c06510 0.10 -1.1 667,831 - PGA1_c06510 0.12 -1.6 667,865 + PGA1_c06510 0.13 +1.7 667,865 + PGA1_c06510 0.13 -0.3 667,865 + PGA1_c06510 0.13 -1.8 667,873 - PGA1_c06510 0.13 -0.7 667,873 - PGA1_c06510 0.13 +0.2 667,873 - PGA1_c06510 0.13 -3.3 667,873 - PGA1_c06510 0.13 +0.9 667,873 - PGA1_c06510 0.13 -0.1 667,873 - PGA1_c06510 0.13 +0.2 667,873 - PGA1_c06510 0.13 -1.3 667,873 - PGA1_c06510 0.13 -0.3 667,873 - PGA1_c06510 0.13 -0.2 667,873 - PGA1_c06510 0.13 -1.9 667,881 - PGA1_c06510 0.13 -1.5 667,919 + PGA1_c06510 0.15 -0.4 667,924 + PGA1_c06510 0.15 +0.3 667,927 - PGA1_c06510 0.15 -0.6 667,929 + PGA1_c06510 0.15 +0.5 667,929 + PGA1_c06510 0.15 -0.9 667,931 + PGA1_c06510 0.15 +0.2 667,937 - PGA1_c06510 0.16 -2.1 667,937 - PGA1_c06510 0.16 -0.2 667,937 - PGA1_c06510 0.16 -1.7 667,937 - PGA1_c06510 0.16 -0.5 667,956 + PGA1_c06510 0.16 +0.5 668,019 - PGA1_c06510 0.19 -1.2 668,036 - PGA1_c06510 0.19 +0.1 668,136 + PGA1_c06510 0.23 -1.6 668,144 - PGA1_c06510 0.24 +0.1 668,144 - PGA1_c06510 0.24 -0.1 668,203 - PGA1_c06510 0.26 -0.8 668,374 + PGA1_c06510 0.32 -0.5 668,422 + PGA1_c06510 0.34 +0.2 668,432 + PGA1_c06510 0.35 -1.0 668,470 + PGA1_c06510 0.36 -0.5 668,470 + PGA1_c06510 0.36 +0.8 668,478 - PGA1_c06510 0.36 +0.4 668,596 - PGA1_c06510 0.41 -1.6 668,669 + PGA1_c06510 0.44 -2.2 668,669 + PGA1_c06510 0.44 -0.4 668,669 + PGA1_c06510 0.44 -1.3 668,677 - PGA1_c06510 0.44 -1.3 668,713 + PGA1_c06510 0.45 +0.9 668,718 + PGA1_c06510 0.46 -2.8 668,723 + PGA1_c06510 0.46 +0.2 668,726 - PGA1_c06510 0.46 -1.0 668,787 - PGA1_c06510 0.48 -0.7 668,837 + PGA1_c06510 0.50 -0.8 668,852 + PGA1_c06510 0.51 -0.8 668,852 + PGA1_c06510 0.51 -0.8 668,852 + PGA1_c06510 0.51 -0.6 668,852 + PGA1_c06510 0.51 -2.7 668,876 - PGA1_c06510 0.52 -2.6 668,943 + PGA1_c06510 0.54 -0.7 669,046 + PGA1_c06510 0.58 -0.7 669,054 - PGA1_c06510 0.59 -0.2 669,119 - PGA1_c06510 0.61 +0.6 669,189 + PGA1_c06510 0.64 -1.1 669,253 - PGA1_c06510 0.66 -1.1 669,328 - PGA1_c06510 0.69 -0.3 669,370 - PGA1_c06510 0.71 -1.1 669,437 - PGA1_c06510 0.73 -0.8 669,446 - PGA1_c06510 0.74 +0.5 669,548 + PGA1_c06510 0.78 +0.6 669,548 + PGA1_c06510 0.78 -0.5 669,667 + PGA1_c06510 0.82 -0.9 669,787 - PGA1_c06510 0.87 -1.6 669,802 - PGA1_c06510 0.87 -1.2 669,852 + PGA1_c06510 0.89 -0.8 669,895 - -1.0 670,184 - -0.9 670,197 + +0.9 670,198 - -0.0 670,267 - -0.4 670,389 + PGA1_c06520 0.18 -0.2 670,389 + PGA1_c06520 0.18 -0.5 670,389 + PGA1_c06520 0.18 +0.4 670,389 + PGA1_c06520 0.18 -0.2 670,397 - PGA1_c06520 0.19 -0.7 670,397 - PGA1_c06520 0.19 +0.2 670,397 - PGA1_c06520 0.19 -1.4 670,397 - PGA1_c06520 0.19 -0.8 670,473 + PGA1_c06520 0.30 +0.4 670,473 - PGA1_c06520 0.30 -0.9 670,481 - PGA1_c06520 0.31 +0.4 670,509 + PGA1_c06520 0.35 +0.2 670,579 - PGA1_c06520 0.46 -0.1 670,722 - PGA1_c06520 0.67 +0.5 670,906 - +0.4 670,906 - -0.1 670,926 + +0.5 670,944 - +0.8 671,011 + -0.2 671,055 + -0.1
Or see this region's nucleotide sequence