Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00170 and PGA1_65p00180 are separated by 354 nucleotides PGA1_65p00180 and PGA1_65p00190 are separated by 21 nucleotides
PGA1_65p00170: PGA1_65p00170 - putative capsular polysaccharide biosynthesis protein, at 22,292 to 24,139
_65p00170
PGA1_65p00180: PGA1_65p00180 - putative sugar transferase, at 24,494 to 25,105
_65p00180
PGA1_65p00190: PGA1_65p00190 - putative NAD dependent epimerase/dehydratase, at 25,127 to 26,176
_65p00190
Position (kb) 24
25
26 Strain fitness (log2 ratio) -1
0
1 at 23.504 kb on - strand, within PGA1_65p00170 at 23.514 kb on - strand, within PGA1_65p00170 at 23.723 kb on - strand, within PGA1_65p00170 at 23.833 kb on - strand, within PGA1_65p00170 at 23.838 kb on - strand, within PGA1_65p00170 at 23.856 kb on - strand, within PGA1_65p00170 at 23.879 kb on + strand, within PGA1_65p00170 at 23.894 kb on - strand, within PGA1_65p00170 at 23.926 kb on + strand, within PGA1_65p00170 at 24.023 kb on + strand at 24.023 kb on + strand at 24.046 kb on - strand at 24.052 kb on + strand at 24.092 kb on + strand at 24.184 kb on + strand at 24.294 kb on + strand at 24.302 kb on - strand at 24.394 kb on - strand at 24.428 kb on - strand at 24.490 kb on + strand at 24.498 kb on - strand at 24.612 kb on + strand, within PGA1_65p00180 at 24.926 kb on + strand, within PGA1_65p00180 at 24.944 kb on + strand, within PGA1_65p00180 at 24.963 kb on - strand, within PGA1_65p00180 at 24.971 kb on - strand, within PGA1_65p00180 at 24.971 kb on - strand, within PGA1_65p00180 at 24.989 kb on - strand, within PGA1_65p00180 at 25.057 kb on + strand at 25.144 kb on - strand at 25.257 kb on + strand, within PGA1_65p00190 at 25.392 kb on - strand, within PGA1_65p00190
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 23,504 - PGA1_65p00170 0.66 -0.5 23,514 - PGA1_65p00170 0.66 +0.4 23,723 - PGA1_65p00170 0.77 +0.5 23,833 - PGA1_65p00170 0.83 +1.4 23,838 - PGA1_65p00170 0.84 +1.0 23,856 - PGA1_65p00170 0.85 -0.2 23,879 + PGA1_65p00170 0.86 -1.2 23,894 - PGA1_65p00170 0.87 -0.4 23,926 + PGA1_65p00170 0.88 +0.4 24,023 + -0.8 24,023 + +0.6 24,046 - +0.3 24,052 + +0.8 24,092 + +1.5 24,184 + +0.7 24,294 + +1.6 24,302 - +0.2 24,394 - +0.4 24,428 - -1.0 24,490 + +0.8 24,498 - +0.6 24,612 + PGA1_65p00180 0.19 +1.1 24,926 + PGA1_65p00180 0.71 -1.6 24,944 + PGA1_65p00180 0.74 -0.0 24,963 - PGA1_65p00180 0.77 -0.6 24,971 - PGA1_65p00180 0.78 +0.2 24,971 - PGA1_65p00180 0.78 -0.3 24,989 - PGA1_65p00180 0.81 +0.5 25,057 + +0.9 25,144 - +1.0 25,257 + PGA1_65p00190 0.12 -0.0 25,392 - PGA1_65p00190 0.25 -1.0
Or see this region's nucleotide sequence