Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35190 and PGA1_c35200 are separated by 115 nucleotides PGA1_c35200 and PGA1_c35210 overlap by 4 nucleotides PGA1_c35210 and PGA1_c35220 overlap by 1 nucleotides PGA1_c35220 and PGA1_c35230 are separated by 21 nucleotides
PGA1_c35190: PGA1_c35190 - putative protein FxsA, at 3,690,768 to 3,691,274
_c35190
PGA1_c35200: PGA1_c35200 - putative transporter protein, at 3,691,390 to 3,692,046
_c35200
PGA1_c35210: PGA1_c35210 - putative membrane-bound lytic murein transglycosylase A, at 3,692,043 to 3,693,098
_c35210
PGA1_c35220: PGA1_c35220 - Uncharacterized protein conserved in bacteria, at 3,693,098 to 3,693,700
_c35220
PGA1_c35230: PGA1_c35230 - Uncharacterized protein conserved in bacteria, at 3,693,722 to 3,694,867
_c35230
Position (kb)
3692
3693
3694 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.198 kb on - strand, within PGA1_c35190 at 3691.209 kb on + strand, within PGA1_c35190 at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.300 kb on + strand at 3691.311 kb on - strand at 3691.316 kb on - strand at 3691.320 kb on + strand at 3691.330 kb on + strand at 3691.395 kb on - strand at 3691.416 kb on + strand at 3691.435 kb on - strand at 3691.460 kb on - strand, within PGA1_c35200 at 3691.468 kb on - strand, within PGA1_c35200 at 3691.472 kb on + strand, within PGA1_c35200 at 3691.480 kb on - strand, within PGA1_c35200 at 3691.488 kb on - strand, within PGA1_c35200 at 3691.600 kb on - strand, within PGA1_c35200 at 3691.645 kb on - strand, within PGA1_c35200 at 3691.689 kb on + strand, within PGA1_c35200 at 3691.749 kb on - strand, within PGA1_c35200 at 3691.794 kb on - strand, within PGA1_c35200 at 3691.818 kb on - strand, within PGA1_c35200 at 3691.858 kb on - strand, within PGA1_c35200 at 3691.878 kb on + strand, within PGA1_c35200 at 3691.910 kb on - strand, within PGA1_c35200 at 3692.129 kb on + strand at 3692.139 kb on + strand at 3692.139 kb on + strand at 3692.154 kb on + strand, within PGA1_c35210 at 3692.175 kb on - strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.281 kb on - strand, within PGA1_c35210 at 3692.335 kb on + strand, within PGA1_c35210 at 3692.343 kb on - strand, within PGA1_c35210 at 3692.380 kb on - strand, within PGA1_c35210 at 3692.390 kb on - strand, within PGA1_c35210 at 3692.409 kb on - strand, within PGA1_c35210 at 3692.469 kb on + strand, within PGA1_c35210 at 3692.482 kb on + strand, within PGA1_c35210 at 3692.482 kb on + strand, within PGA1_c35210 at 3692.601 kb on + strand, within PGA1_c35210 at 3692.601 kb on + strand, within PGA1_c35210 at 3692.609 kb on - strand, within PGA1_c35210 at 3692.609 kb on - strand, within PGA1_c35210 at 3692.636 kb on + strand, within PGA1_c35210 at 3692.641 kb on + strand, within PGA1_c35210 at 3692.681 kb on + strand, within PGA1_c35210 at 3692.681 kb on + strand, within PGA1_c35210 at 3692.681 kb on + strand, within PGA1_c35210 at 3692.686 kb on + strand, within PGA1_c35210 at 3692.689 kb on - strand, within PGA1_c35210 at 3692.689 kb on - strand, within PGA1_c35210 at 3692.689 kb on - strand, within PGA1_c35210 at 3692.689 kb on - strand, within PGA1_c35210 at 3692.689 kb on - strand, within PGA1_c35210 at 3692.694 kb on - strand, within PGA1_c35210 at 3692.694 kb on - strand, within PGA1_c35210 at 3692.694 kb on - strand, within PGA1_c35210 at 3692.694 kb on - strand, within PGA1_c35210 at 3692.694 kb on - strand, within PGA1_c35210 at 3692.699 kb on - strand, within PGA1_c35210 at 3692.704 kb on - strand, within PGA1_c35210 at 3692.704 kb on - strand, within PGA1_c35210 at 3692.893 kb on - strand, within PGA1_c35210 at 3693.092 kb on + strand at 3693.094 kb on + strand at 3693.100 kb on - strand at 3693.117 kb on + strand at 3693.208 kb on + strand, within PGA1_c35220 at 3693.213 kb on + strand, within PGA1_c35220 at 3693.247 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.258 kb on + strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.266 kb on - strand, within PGA1_c35220 at 3693.279 kb on + strand, within PGA1_c35220 at 3693.280 kb on + strand, within PGA1_c35220 at 3693.291 kb on + strand, within PGA1_c35220 at 3693.306 kb on + strand, within PGA1_c35220 at 3693.338 kb on - strand, within PGA1_c35220 at 3693.390 kb on - strand, within PGA1_c35220 at 3693.619 kb on + strand, within PGA1_c35220 at 3693.738 kb on + strand at 3693.783 kb on + strand at 3693.783 kb on + strand at 3693.783 kb on + strand at 3693.864 kb on - strand, within PGA1_c35230 at 3693.895 kb on - strand, within PGA1_c35230 at 3693.976 kb on + strand, within PGA1_c35230 at 3693.976 kb on + strand, within PGA1_c35230 at 3694.029 kb on + strand, within PGA1_c35230 at 3694.047 kb on - strand, within PGA1_c35230
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 3,691,184 + PGA1_c35190 0.82 -0.6 3,691,184 + PGA1_c35190 0.82 +1.0 3,691,184 + PGA1_c35190 0.82 +0.1 3,691,198 - PGA1_c35190 0.85 +0.5 3,691,209 + PGA1_c35190 0.87 -0.8 3,691,292 - +0.2 3,691,292 - -0.7 3,691,292 - +0.1 3,691,300 + -0.9 3,691,311 - +0.7 3,691,316 - -1.9 3,691,320 + -0.5 3,691,330 + -1.4 3,691,395 - -1.0 3,691,416 + -0.8 3,691,435 - -1.2 3,691,460 - PGA1_c35200 0.11 -3.0 3,691,468 - PGA1_c35200 0.12 -1.7 3,691,472 + PGA1_c35200 0.12 -0.6 3,691,480 - PGA1_c35200 0.14 -1.9 3,691,488 - PGA1_c35200 0.15 -1.2 3,691,600 - PGA1_c35200 0.32 +0.2 3,691,645 - PGA1_c35200 0.39 -2.0 3,691,689 + PGA1_c35200 0.46 +0.1 3,691,749 - PGA1_c35200 0.55 -1.7 3,691,794 - PGA1_c35200 0.61 -2.7 3,691,818 - PGA1_c35200 0.65 -1.6 3,691,858 - PGA1_c35200 0.71 -0.5 3,691,878 + PGA1_c35200 0.74 -1.3 3,691,910 - PGA1_c35200 0.79 -2.6 3,692,129 + +0.5 3,692,139 + -1.7 3,692,139 + -0.2 3,692,154 + PGA1_c35210 0.11 +0.3 3,692,175 - PGA1_c35210 0.12 +0.6 3,692,273 + PGA1_c35210 0.22 -1.9 3,692,273 + PGA1_c35210 0.22 -0.5 3,692,273 + PGA1_c35210 0.22 -2.0 3,692,273 + PGA1_c35210 0.22 +0.1 3,692,273 + PGA1_c35210 0.22 +0.9 3,692,273 + PGA1_c35210 0.22 -0.3 3,692,273 + PGA1_c35210 0.22 -0.6 3,692,273 + PGA1_c35210 0.22 -0.4 3,692,273 + PGA1_c35210 0.22 +0.9 3,692,273 + PGA1_c35210 0.22 -0.8 3,692,273 + PGA1_c35210 0.22 +1.4 3,692,273 + PGA1_c35210 0.22 +0.8 3,692,273 + PGA1_c35210 0.22 -0.5 3,692,273 + PGA1_c35210 0.22 -2.0 3,692,273 + PGA1_c35210 0.22 -0.0 3,692,273 + PGA1_c35210 0.22 -0.4 3,692,273 + PGA1_c35210 0.22 +1.1 3,692,281 - PGA1_c35210 0.23 -0.3 3,692,281 - PGA1_c35210 0.23 -1.6 3,692,281 - PGA1_c35210 0.23 +0.8 3,692,281 - PGA1_c35210 0.23 +0.0 3,692,281 - PGA1_c35210 0.23 +0.0 3,692,281 - PGA1_c35210 0.23 -0.5 3,692,281 - PGA1_c35210 0.23 +0.4 3,692,281 - PGA1_c35210 0.23 +0.8 3,692,281 - PGA1_c35210 0.23 -0.7 3,692,281 - PGA1_c35210 0.23 +0.6 3,692,281 - PGA1_c35210 0.23 -0.2 3,692,281 - PGA1_c35210 0.23 -0.1 3,692,335 + PGA1_c35210 0.28 +0.1 3,692,343 - PGA1_c35210 0.28 +0.5 3,692,380 - PGA1_c35210 0.32 +0.1 3,692,390 - PGA1_c35210 0.33 +0.1 3,692,409 - PGA1_c35210 0.35 +1.2 3,692,469 + PGA1_c35210 0.40 -0.9 3,692,482 + PGA1_c35210 0.42 -0.2 3,692,482 + PGA1_c35210 0.42 -0.2 3,692,601 + PGA1_c35210 0.53 +0.3 3,692,601 + PGA1_c35210 0.53 -0.5 3,692,609 - PGA1_c35210 0.54 -0.9 3,692,609 - PGA1_c35210 0.54 -0.2 3,692,636 + PGA1_c35210 0.56 -1.0 3,692,641 + PGA1_c35210 0.57 +0.3 3,692,681 + PGA1_c35210 0.60 +1.0 3,692,681 + PGA1_c35210 0.60 -0.4 3,692,681 + PGA1_c35210 0.60 -0.3 3,692,686 + PGA1_c35210 0.61 -0.8 3,692,689 - PGA1_c35210 0.61 +0.3 3,692,689 - PGA1_c35210 0.61 +0.4 3,692,689 - PGA1_c35210 0.61 +0.7 3,692,689 - PGA1_c35210 0.61 -0.2 3,692,689 - PGA1_c35210 0.61 +0.7 3,692,694 - PGA1_c35210 0.62 -0.6 3,692,694 - PGA1_c35210 0.62 -1.0 3,692,694 - PGA1_c35210 0.62 +0.5 3,692,694 - PGA1_c35210 0.62 +0.1 3,692,694 - PGA1_c35210 0.62 +0.6 3,692,699 - PGA1_c35210 0.62 -0.3 3,692,704 - PGA1_c35210 0.63 -0.2 3,692,704 - PGA1_c35210 0.63 -0.3 3,692,893 - PGA1_c35210 0.80 +1.2 3,693,092 + -1.7 3,693,094 + -0.1 3,693,100 - -0.9 3,693,117 + -0.3 3,693,208 + PGA1_c35220 0.18 -1.2 3,693,213 + PGA1_c35220 0.19 -0.2 3,693,247 + PGA1_c35220 0.25 +0.0 3,693,258 + PGA1_c35220 0.27 -1.4 3,693,258 + PGA1_c35220 0.27 -0.1 3,693,258 + PGA1_c35220 0.27 +0.1 3,693,258 + PGA1_c35220 0.27 -0.2 3,693,258 + PGA1_c35220 0.27 -0.2 3,693,258 + PGA1_c35220 0.27 +0.5 3,693,258 + PGA1_c35220 0.27 -0.6 3,693,258 + PGA1_c35220 0.27 -0.7 3,693,258 + PGA1_c35220 0.27 -0.2 3,693,258 + PGA1_c35220 0.27 -0.0 3,693,266 - PGA1_c35220 0.28 -0.0 3,693,266 - PGA1_c35220 0.28 +0.0 3,693,266 - PGA1_c35220 0.28 +0.4 3,693,266 - PGA1_c35220 0.28 -1.0 3,693,266 - PGA1_c35220 0.28 -0.0 3,693,266 - PGA1_c35220 0.28 -0.4 3,693,266 - PGA1_c35220 0.28 -0.4 3,693,266 - PGA1_c35220 0.28 +0.6 3,693,266 - PGA1_c35220 0.28 +0.5 3,693,266 - PGA1_c35220 0.28 -1.9 3,693,266 - PGA1_c35220 0.28 +0.5 3,693,266 - PGA1_c35220 0.28 -0.7 3,693,279 + PGA1_c35220 0.30 -1.2 3,693,280 + PGA1_c35220 0.30 +0.2 3,693,291 + PGA1_c35220 0.32 +0.4 3,693,306 + PGA1_c35220 0.34 +0.2 3,693,338 - PGA1_c35220 0.40 -0.3 3,693,390 - PGA1_c35220 0.48 +1.0 3,693,619 + PGA1_c35220 0.86 -0.9 3,693,738 + -0.8 3,693,783 + +1.0 3,693,783 + +1.3 3,693,783 + -0.2 3,693,864 - PGA1_c35230 0.12 -0.3 3,693,895 - PGA1_c35230 0.15 -1.4 3,693,976 + PGA1_c35230 0.22 -0.1 3,693,976 + PGA1_c35230 0.22 -0.2 3,694,029 + PGA1_c35230 0.27 +0.0 3,694,047 - PGA1_c35230 0.28 +0.4
Or see this region's nucleotide sequence