Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24190 and PGA1_c24200 are separated by 181 nucleotides PGA1_c24200 and PGA1_c24210 are separated by 90 nucleotides
PGA1_c24190: PGA1_c24190 - ATP-dependent RNA helicase HrpB, at 2,522,356 to 2,524,860
_c24190
PGA1_c24200: PGA1_c24200 - putative NUDIX hydrolase, at 2,525,042 to 2,525,500
_c24200
PGA1_c24210: PGA1_c24210 - putative phosphate transporter, at 2,525,591 to 2,527,072
_c24210
Position (kb)
2525
2526 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2524.121 kb on + strand, within PGA1_c24190 at 2524.121 kb on + strand, within PGA1_c24190 at 2524.121 kb on + strand, within PGA1_c24190 at 2524.126 kb on - strand, within PGA1_c24190 at 2524.129 kb on - strand, within PGA1_c24190 at 2524.134 kb on - strand, within PGA1_c24190 at 2524.152 kb on + strand, within PGA1_c24190 at 2524.152 kb on + strand, within PGA1_c24190 at 2524.177 kb on - strand, within PGA1_c24190 at 2524.187 kb on - strand, within PGA1_c24190 at 2524.226 kb on - strand, within PGA1_c24190 at 2524.228 kb on - strand, within PGA1_c24190 at 2524.251 kb on - strand, within PGA1_c24190 at 2524.298 kb on - strand, within PGA1_c24190 at 2524.298 kb on - strand, within PGA1_c24190 at 2524.315 kb on - strand, within PGA1_c24190 at 2524.507 kb on + strand, within PGA1_c24190 at 2524.507 kb on + strand, within PGA1_c24190 at 2524.515 kb on - strand, within PGA1_c24190 at 2524.544 kb on - strand, within PGA1_c24190 at 2524.590 kb on - strand, within PGA1_c24190 at 2524.637 kb on + strand at 2524.700 kb on - strand at 2524.724 kb on + strand at 2524.732 kb on - strand at 2524.762 kb on + strand at 2524.770 kb on - strand at 2524.800 kb on + strand at 2524.800 kb on + strand at 2524.805 kb on + strand at 2524.805 kb on + strand at 2524.808 kb on - strand at 2524.868 kb on - strand at 2524.935 kb on + strand at 2524.943 kb on - strand at 2524.944 kb on + strand at 2525.024 kb on + strand at 2525.087 kb on + strand at 2525.146 kb on + strand, within PGA1_c24200 at 2525.211 kb on - strand, within PGA1_c24200 at 2525.226 kb on - strand, within PGA1_c24200 at 2525.253 kb on + strand, within PGA1_c24200 at 2525.299 kb on + strand, within PGA1_c24200 at 2525.370 kb on - strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.400 kb on + strand, within PGA1_c24200 at 2525.408 kb on - strand, within PGA1_c24200 at 2525.408 kb on - strand, within PGA1_c24200 at 2525.410 kb on + strand, within PGA1_c24200 at 2525.431 kb on - strand, within PGA1_c24200 at 2525.453 kb on + strand, within PGA1_c24200 at 2525.713 kb on + strand at 2525.716 kb on - strand at 2525.845 kb on + strand, within PGA1_c24210 at 2525.853 kb on - strand, within PGA1_c24210 at 2525.878 kb on + strand, within PGA1_c24210 at 2525.878 kb on + strand, within PGA1_c24210 at 2525.886 kb on - strand, within PGA1_c24210 at 2526.101 kb on + strand, within PGA1_c24210 at 2526.341 kb on - strand, within PGA1_c24210 at 2526.361 kb on - strand, within PGA1_c24210 at 2526.402 kb on + strand, within PGA1_c24210 at 2526.402 kb on - strand, within PGA1_c24210 at 2526.405 kb on - strand, within PGA1_c24210 at 2526.429 kb on - strand, within PGA1_c24210 at 2526.445 kb on + strand, within PGA1_c24210 at 2526.445 kb on + strand, within PGA1_c24210 at 2526.465 kb on - strand, within PGA1_c24210
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 2,524,121 + PGA1_c24190 0.70 -2.0 2,524,121 + PGA1_c24190 0.70 -0.1 2,524,121 + PGA1_c24190 0.70 +0.5 2,524,126 - PGA1_c24190 0.71 -0.5 2,524,129 - PGA1_c24190 0.71 -0.2 2,524,134 - PGA1_c24190 0.71 -0.9 2,524,152 + PGA1_c24190 0.72 -0.3 2,524,152 + PGA1_c24190 0.72 -2.9 2,524,177 - PGA1_c24190 0.73 +0.5 2,524,187 - PGA1_c24190 0.73 +0.3 2,524,226 - PGA1_c24190 0.75 +0.4 2,524,228 - PGA1_c24190 0.75 -1.0 2,524,251 - PGA1_c24190 0.76 -2.0 2,524,298 - PGA1_c24190 0.78 +0.7 2,524,298 - PGA1_c24190 0.78 -1.2 2,524,315 - PGA1_c24190 0.78 -1.8 2,524,507 + PGA1_c24190 0.86 +0.1 2,524,507 + PGA1_c24190 0.86 +0.8 2,524,515 - PGA1_c24190 0.86 -0.3 2,524,544 - PGA1_c24190 0.87 +1.2 2,524,590 - PGA1_c24190 0.89 -0.1 2,524,637 + -2.4 2,524,700 - -1.3 2,524,724 + +0.3 2,524,732 - +1.1 2,524,762 + +0.3 2,524,770 - -0.1 2,524,800 + +1.5 2,524,800 + -0.5 2,524,805 + +0.7 2,524,805 + -0.1 2,524,808 - -0.0 2,524,868 - +0.5 2,524,935 + +0.0 2,524,943 - -0.1 2,524,944 + +1.5 2,525,024 + +0.2 2,525,087 + -0.8 2,525,146 + PGA1_c24200 0.23 -3.0 2,525,211 - PGA1_c24200 0.37 -0.5 2,525,226 - PGA1_c24200 0.40 +0.8 2,525,253 + PGA1_c24200 0.46 -2.0 2,525,299 + PGA1_c24200 0.56 -1.4 2,525,370 - PGA1_c24200 0.71 +0.1 2,525,400 + PGA1_c24200 0.78 -1.4 2,525,400 + PGA1_c24200 0.78 -1.2 2,525,400 + PGA1_c24200 0.78 -0.4 2,525,400 + PGA1_c24200 0.78 +0.5 2,525,400 + PGA1_c24200 0.78 -1.4 2,525,400 + PGA1_c24200 0.78 -2.1 2,525,400 + PGA1_c24200 0.78 -1.8 2,525,408 - PGA1_c24200 0.80 +0.7 2,525,408 - PGA1_c24200 0.80 -1.1 2,525,410 + PGA1_c24200 0.80 -0.7 2,525,431 - PGA1_c24200 0.85 -0.2 2,525,453 + PGA1_c24200 0.90 -0.3 2,525,713 + +0.5 2,525,716 - +0.3 2,525,845 + PGA1_c24210 0.17 +0.2 2,525,853 - PGA1_c24210 0.18 -1.7 2,525,878 + PGA1_c24210 0.19 +1.3 2,525,878 + PGA1_c24210 0.19 +0.1 2,525,886 - PGA1_c24210 0.20 -0.2 2,526,101 + PGA1_c24210 0.34 -2.7 2,526,341 - PGA1_c24210 0.51 +0.5 2,526,361 - PGA1_c24210 0.52 -0.7 2,526,402 + PGA1_c24210 0.55 -0.0 2,526,402 - PGA1_c24210 0.55 -1.1 2,526,405 - PGA1_c24210 0.55 -0.6 2,526,429 - PGA1_c24210 0.57 -0.3 2,526,445 + PGA1_c24210 0.58 -0.5 2,526,445 + PGA1_c24210 0.58 +0.9 2,526,465 - PGA1_c24210 0.59 +0.8
Or see this region's nucleotide sequence