Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01820 and PGA1_c01830 are separated by 147 nucleotides PGA1_c01830 and PGA1_c01840 are separated by 593 nucleotides
PGA1_c01820: PGA1_c01820 - Predicted permeases, at 166,389 to 167,333
_c01820
PGA1_c01830: PGA1_c01830 - Uncharacterized conserved small protein, at 167,481 to 167,696
_c01830
PGA1_c01840: PGA1_c01840 - transmembrane ion channel-domain containing protein, at 168,290 to 168,802
_c01840
Position (kb)
167
168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 166.494 kb on - strand, within PGA1_c01820 at 166.513 kb on + strand, within PGA1_c01820 at 166.521 kb on - strand, within PGA1_c01820 at 166.521 kb on - strand, within PGA1_c01820 at 166.546 kb on - strand, within PGA1_c01820 at 166.555 kb on + strand, within PGA1_c01820 at 166.590 kb on - strand, within PGA1_c01820 at 166.592 kb on - strand, within PGA1_c01820 at 166.660 kb on + strand, within PGA1_c01820 at 166.686 kb on - strand, within PGA1_c01820 at 166.693 kb on + strand, within PGA1_c01820 at 166.693 kb on + strand, within PGA1_c01820 at 166.701 kb on - strand, within PGA1_c01820 at 166.701 kb on - strand, within PGA1_c01820 at 166.703 kb on + strand, within PGA1_c01820 at 166.739 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.758 kb on - strand, within PGA1_c01820 at 166.766 kb on - strand, within PGA1_c01820 at 166.766 kb on - strand, within PGA1_c01820 at 166.795 kb on + strand, within PGA1_c01820 at 166.821 kb on - strand, within PGA1_c01820 at 166.831 kb on - strand, within PGA1_c01820 at 166.835 kb on + strand, within PGA1_c01820 at 166.929 kb on - strand, within PGA1_c01820 at 166.942 kb on + strand, within PGA1_c01820 at 166.972 kb on - strand, within PGA1_c01820 at 166.979 kb on + strand, within PGA1_c01820 at 166.983 kb on + strand, within PGA1_c01820 at 166.996 kb on - strand, within PGA1_c01820 at 167.026 kb on + strand, within PGA1_c01820 at 167.040 kb on - strand, within PGA1_c01820 at 167.049 kb on + strand, within PGA1_c01820 at 167.063 kb on - strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.165 kb on + strand, within PGA1_c01820 at 167.191 kb on - strand, within PGA1_c01820 at 167.193 kb on + strand, within PGA1_c01820 at 167.196 kb on - strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.319 kb on - strand at 167.353 kb on - strand at 167.366 kb on + strand at 167.366 kb on + strand at 167.471 kb on + strand at 167.487 kb on - strand at 167.528 kb on - strand, within PGA1_c01830 at 167.558 kb on + strand, within PGA1_c01830 at 167.593 kb on - strand, within PGA1_c01830 at 167.596 kb on + strand, within PGA1_c01830 at 167.623 kb on - strand, within PGA1_c01830 at 167.744 kb on + strand at 167.910 kb on + strand at 167.929 kb on + strand at 167.942 kb on - strand at 167.952 kb on + strand at 167.960 kb on - strand at 167.960 kb on - strand at 167.960 kb on - strand at 168.018 kb on - strand at 168.042 kb on + strand at 168.048 kb on + strand at 168.048 kb on + strand at 168.089 kb on - strand at 168.089 kb on - strand at 168.113 kb on + strand at 168.113 kb on + strand at 168.113 kb on + strand at 168.115 kb on + strand at 168.121 kb on - strand at 168.123 kb on - strand at 168.138 kb on - strand at 168.219 kb on + strand at 168.272 kb on + strand at 168.376 kb on - strand, within PGA1_c01840 at 168.430 kb on + strand, within PGA1_c01840 at 168.435 kb on + strand, within PGA1_c01840 at 168.435 kb on + strand, within PGA1_c01840 at 168.443 kb on - strand, within PGA1_c01840 at 168.452 kb on + strand, within PGA1_c01840 at 168.482 kb on + strand, within PGA1_c01840 at 168.482 kb on + strand, within PGA1_c01840 at 168.490 kb on - strand, within PGA1_c01840 at 168.491 kb on + strand, within PGA1_c01840 at 168.497 kb on + strand, within PGA1_c01840 at 168.499 kb on - strand, within PGA1_c01840 at 168.499 kb on - strand, within PGA1_c01840 at 168.505 kb on - strand, within PGA1_c01840 at 168.511 kb on + strand, within PGA1_c01840 at 168.519 kb on - strand, within PGA1_c01840 at 168.519 kb on - strand, within PGA1_c01840 at 168.543 kb on + strand, within PGA1_c01840 at 168.543 kb on + strand, within PGA1_c01840 at 168.548 kb on + strand, within PGA1_c01840 at 168.548 kb on - strand, within PGA1_c01840 at 168.551 kb on - strand, within PGA1_c01840 at 168.551 kb on - strand, within PGA1_c01840 at 168.551 kb on - strand, within PGA1_c01840 at 168.551 kb on - strand, within PGA1_c01840 at 168.607 kb on + strand, within PGA1_c01840 at 168.619 kb on + strand, within PGA1_c01840 at 168.626 kb on - strand, within PGA1_c01840 at 168.664 kb on + strand, within PGA1_c01840 at 168.664 kb on + strand, within PGA1_c01840 at 168.664 kb on + strand, within PGA1_c01840 at 168.664 kb on + strand, within PGA1_c01840 at 168.672 kb on - strand, within PGA1_c01840 at 168.672 kb on - strand, within PGA1_c01840 at 168.688 kb on + strand, within PGA1_c01840
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 166,494 - PGA1_c01820 0.11 -1.0 166,513 + PGA1_c01820 0.13 -1.7 166,521 - PGA1_c01820 0.14 +0.4 166,521 - PGA1_c01820 0.14 +1.1 166,546 - PGA1_c01820 0.17 +1.4 166,555 + PGA1_c01820 0.18 +0.1 166,590 - PGA1_c01820 0.21 +0.7 166,592 - PGA1_c01820 0.21 +0.7 166,660 + PGA1_c01820 0.29 +0.4 166,686 - PGA1_c01820 0.31 -0.3 166,693 + PGA1_c01820 0.32 -0.4 166,693 + PGA1_c01820 0.32 +0.6 166,701 - PGA1_c01820 0.33 -2.8 166,701 - PGA1_c01820 0.33 -0.5 166,703 + PGA1_c01820 0.33 +0.9 166,739 - PGA1_c01820 0.37 -0.7 166,744 - PGA1_c01820 0.38 +0.5 166,744 - PGA1_c01820 0.38 -0.3 166,744 - PGA1_c01820 0.38 -0.7 166,758 - PGA1_c01820 0.39 -0.7 166,766 - PGA1_c01820 0.40 +0.1 166,766 - PGA1_c01820 0.40 -0.7 166,795 + PGA1_c01820 0.43 -0.2 166,821 - PGA1_c01820 0.46 +0.3 166,831 - PGA1_c01820 0.47 +0.7 166,835 + PGA1_c01820 0.47 -1.1 166,929 - PGA1_c01820 0.57 +0.6 166,942 + PGA1_c01820 0.59 +0.0 166,972 - PGA1_c01820 0.62 +2.5 166,979 + PGA1_c01820 0.62 -0.5 166,983 + PGA1_c01820 0.63 -0.9 166,996 - PGA1_c01820 0.64 +0.3 167,026 + PGA1_c01820 0.67 +1.1 167,040 - PGA1_c01820 0.69 -0.7 167,049 + PGA1_c01820 0.70 +1.6 167,063 - PGA1_c01820 0.71 -0.9 167,132 + PGA1_c01820 0.79 +0.7 167,132 + PGA1_c01820 0.79 -0.6 167,132 + PGA1_c01820 0.79 -0.1 167,140 - PGA1_c01820 0.79 -0.1 167,140 - PGA1_c01820 0.79 -0.3 167,140 - PGA1_c01820 0.79 -0.3 167,165 + PGA1_c01820 0.82 -0.1 167,191 - PGA1_c01820 0.85 -0.3 167,193 + PGA1_c01820 0.85 +1.0 167,196 - PGA1_c01820 0.85 +0.5 167,233 + PGA1_c01820 0.89 +0.8 167,233 + PGA1_c01820 0.89 +0.2 167,233 + PGA1_c01820 0.89 +0.9 167,301 + -0.1 167,301 + +0.6 167,301 + -0.7 167,301 + +1.7 167,301 + -0.3 167,301 + +0.7 167,309 - -1.9 167,309 - +0.1 167,309 - +0.0 167,309 - +0.9 167,319 - +0.0 167,353 - -2.4 167,366 + -0.6 167,366 + -1.3 167,471 + -0.1 167,487 - -1.6 167,528 - PGA1_c01830 0.22 -1.9 167,558 + PGA1_c01830 0.36 -1.0 167,593 - PGA1_c01830 0.52 -0.9 167,596 + PGA1_c01830 0.53 -1.6 167,623 - PGA1_c01830 0.66 -3.2 167,744 + +0.6 167,910 + +0.3 167,929 + +0.6 167,942 - -1.4 167,952 + +0.1 167,960 - -0.4 167,960 - -0.4 167,960 - +0.4 168,018 - +0.3 168,042 + -0.2 168,048 + -0.6 168,048 + -1.9 168,089 - +0.8 168,089 - -3.2 168,113 + +1.5 168,113 + -0.1 168,113 + +0.9 168,115 + +0.4 168,121 - +0.8 168,123 - -1.4 168,138 - -1.2 168,219 + -0.3 168,272 + +0.9 168,376 - PGA1_c01840 0.17 -1.9 168,430 + PGA1_c01840 0.27 +1.3 168,435 + PGA1_c01840 0.28 -0.6 168,435 + PGA1_c01840 0.28 +0.2 168,443 - PGA1_c01840 0.30 +0.3 168,452 + PGA1_c01840 0.32 +0.8 168,482 + PGA1_c01840 0.37 -1.1 168,482 + PGA1_c01840 0.37 +0.1 168,490 - PGA1_c01840 0.39 +1.7 168,491 + PGA1_c01840 0.39 -0.2 168,497 + PGA1_c01840 0.40 -0.4 168,499 - PGA1_c01840 0.41 +0.6 168,499 - PGA1_c01840 0.41 +1.1 168,505 - PGA1_c01840 0.42 +0.8 168,511 + PGA1_c01840 0.43 -0.3 168,519 - PGA1_c01840 0.45 -0.6 168,519 - PGA1_c01840 0.45 -0.0 168,543 + PGA1_c01840 0.49 +0.5 168,543 + PGA1_c01840 0.49 +1.4 168,548 + PGA1_c01840 0.50 -0.7 168,548 - PGA1_c01840 0.50 +0.6 168,551 - PGA1_c01840 0.51 -0.1 168,551 - PGA1_c01840 0.51 +0.7 168,551 - PGA1_c01840 0.51 +0.5 168,551 - PGA1_c01840 0.51 +0.2 168,607 + PGA1_c01840 0.62 -1.4 168,619 + PGA1_c01840 0.64 -0.7 168,626 - PGA1_c01840 0.65 -1.7 168,664 + PGA1_c01840 0.73 -0.5 168,664 + PGA1_c01840 0.73 -0.6 168,664 + PGA1_c01840 0.73 +0.4 168,664 + PGA1_c01840 0.73 -0.2 168,672 - PGA1_c01840 0.74 +0.3 168,672 - PGA1_c01840 0.74 -0.3 168,688 + PGA1_c01840 0.78 -0.7
Or see this region's nucleotide sequence