Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13160 and PGA1_c13170 are separated by 29 nucleotides PGA1_c13170 and PGA1_c13180 overlap by 4 nucleotides PGA1_c13180 and PGA1_c13190 are separated by 11 nucleotides PGA1_c13190 and PGA1_c13200 are separated by 9 nucleotides
PGA1_c13160: PGA1_c13160 - mannitol 2-dehydrogenase MtlK, at 1,361,569 to 1,363,035
_c13160
PGA1_c13170: PGA1_c13170 - Sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 1,363,065 to 1,363,838
_c13170
PGA1_c13180: PGA1_c13180 - ABC transporter for D-Sorbitol, ATPase component (from data) , at 1,363,835 to 1,364,839
_c13180
PGA1_c13190: PGA1_c13190 - ABC transporter for D-Sorbitol, permease component 1 (from data) , at 1,364,851 to 1,365,681
_c13190
PGA1_c13200: PGA1_c13200 - ABC transporter for D-Sorbitol, permease component 2 (from data) , at 1,365,691 to 1,366,557
_c13200
Position (kb)
1363
1364
1365 Strain fitness (log2 ratio)
-2
-1
0
1 at 1362.885 kb on + strand, within PGA1_c13160 at 1362.915 kb on - strand at 1362.969 kb on - strand at 1362.981 kb on - strand at 1362.990 kb on - strand at 1362.995 kb on + strand at 1363.052 kb on - strand at 1363.057 kb on - strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.106 kb on - strand at 1363.106 kb on - strand at 1363.106 kb on - strand at 1363.107 kb on - strand at 1363.107 kb on - strand at 1363.131 kb on + strand at 1363.160 kb on + strand, within PGA1_c13170 at 1363.176 kb on - strand, within PGA1_c13170 at 1363.225 kb on + strand, within PGA1_c13170 at 1363.263 kb on + strand, within PGA1_c13170 at 1363.263 kb on + strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.271 kb on - strand, within PGA1_c13170 at 1363.342 kb on - strand, within PGA1_c13170 at 1363.351 kb on + strand, within PGA1_c13170 at 1363.444 kb on + strand, within PGA1_c13170 at 1363.472 kb on - strand, within PGA1_c13170 at 1363.543 kb on + strand, within PGA1_c13170 at 1363.551 kb on - strand, within PGA1_c13170 at 1363.569 kb on - strand, within PGA1_c13170 at 1363.652 kb on - strand, within PGA1_c13170 at 1363.652 kb on - strand, within PGA1_c13170 at 1363.699 kb on + strand, within PGA1_c13170 at 1363.707 kb on - strand, within PGA1_c13170 at 1363.714 kb on - strand, within PGA1_c13170 at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.801 kb on + strand at 1363.809 kb on - strand at 1363.809 kb on - strand at 1363.822 kb on + strand at 1363.890 kb on - strand at 1364.093 kb on - strand, within PGA1_c13180 at 1364.098 kb on - strand, within PGA1_c13180 at 1364.169 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.190 kb on + strand, within PGA1_c13180 at 1364.258 kb on - strand, within PGA1_c13180 at 1364.349 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.433 kb on + strand, within PGA1_c13180 at 1364.436 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.441 kb on - strand, within PGA1_c13180 at 1364.482 kb on - strand, within PGA1_c13180 at 1364.665 kb on + strand, within PGA1_c13180 at 1364.681 kb on + strand, within PGA1_c13180 at 1364.823 kb on + strand at 1364.831 kb on - strand at 1364.831 kb on - strand at 1364.833 kb on + strand at 1364.841 kb on - strand at 1364.842 kb on - strand at 1364.869 kb on + strand at 1364.869 kb on + strand at 1364.869 kb on + strand at 1364.877 kb on - strand at 1364.877 kb on - strand at 1364.902 kb on - strand at 1364.919 kb on - strand at 1364.953 kb on - strand, within PGA1_c13190 at 1364.957 kb on + strand, within PGA1_c13190 at 1364.957 kb on + strand, within PGA1_c13190 at 1364.960 kb on - strand, within PGA1_c13190 at 1364.965 kb on - strand, within PGA1_c13190 at 1364.970 kb on - strand, within PGA1_c13190 at 1365.061 kb on - strand, within PGA1_c13190 at 1365.082 kb on + strand, within PGA1_c13190 at 1365.083 kb on + strand, within PGA1_c13190 at 1365.119 kb on + strand, within PGA1_c13190 at 1365.141 kb on + strand, within PGA1_c13190 at 1365.145 kb on + strand, within PGA1_c13190 at 1365.149 kb on + strand, within PGA1_c13190 at 1365.172 kb on - strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.199 kb on + strand, within PGA1_c13190 at 1365.207 kb on - strand, within PGA1_c13190 at 1365.310 kb on + strand, within PGA1_c13190 at 1365.364 kb on - strand, within PGA1_c13190 at 1365.551 kb on + strand, within PGA1_c13190 at 1365.559 kb on - strand, within PGA1_c13190 at 1365.561 kb on + strand, within PGA1_c13190 at 1365.569 kb on + strand, within PGA1_c13190 at 1365.580 kb on + strand, within PGA1_c13190 at 1365.580 kb on + strand, within PGA1_c13190 at 1365.590 kb on + strand, within PGA1_c13190 at 1365.590 kb on + strand, within PGA1_c13190 at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.626 kb on + strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.634 kb on - strand at 1365.639 kb on - strand at 1365.649 kb on - strand at 1365.690 kb on + strand at 1365.826 kb on + strand, within PGA1_c13200
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 5 mM remove 1,362,885 + PGA1_c13160 0.90 -0.4 1,362,915 - +0.2 1,362,969 - +0.0 1,362,981 - -1.0 1,362,990 - -1.6 1,362,995 + +0.6 1,363,052 - +0.9 1,363,057 - -0.3 1,363,098 + -1.0 1,363,098 + +0.3 1,363,098 + -0.4 1,363,106 - +0.7 1,363,106 - +0.2 1,363,106 - +1.3 1,363,107 - -2.3 1,363,107 - +0.3 1,363,131 + -0.7 1,363,160 + PGA1_c13170 0.12 +0.4 1,363,176 - PGA1_c13170 0.14 -0.4 1,363,225 + PGA1_c13170 0.21 -0.9 1,363,263 + PGA1_c13170 0.26 +0.9 1,363,263 + PGA1_c13170 0.26 -1.7 1,363,271 - PGA1_c13170 0.27 -0.9 1,363,271 - PGA1_c13170 0.27 -0.2 1,363,271 - PGA1_c13170 0.27 -0.2 1,363,342 - PGA1_c13170 0.36 -0.2 1,363,351 + PGA1_c13170 0.37 +1.0 1,363,444 + PGA1_c13170 0.49 +0.7 1,363,472 - PGA1_c13170 0.53 -1.6 1,363,543 + PGA1_c13170 0.62 -0.7 1,363,551 - PGA1_c13170 0.63 +0.1 1,363,569 - PGA1_c13170 0.65 -1.1 1,363,652 - PGA1_c13170 0.76 -1.3 1,363,652 - PGA1_c13170 0.76 +0.5 1,363,699 + PGA1_c13170 0.82 -0.1 1,363,707 - PGA1_c13170 0.83 -0.3 1,363,714 - PGA1_c13170 0.84 -0.5 1,363,801 + +0.4 1,363,801 + -0.7 1,363,801 + +1.2 1,363,801 + +0.7 1,363,801 + -0.1 1,363,801 + -1.1 1,363,801 + -0.8 1,363,809 - -0.6 1,363,809 - -1.2 1,363,822 + -2.5 1,363,890 - +0.5 1,364,093 - PGA1_c13180 0.26 +0.3 1,364,098 - PGA1_c13180 0.26 -0.9 1,364,169 + PGA1_c13180 0.33 +0.1 1,364,190 + PGA1_c13180 0.35 -0.2 1,364,190 + PGA1_c13180 0.35 +0.0 1,364,258 - PGA1_c13180 0.42 +0.8 1,364,349 + PGA1_c13180 0.51 -0.9 1,364,433 + PGA1_c13180 0.60 -0.3 1,364,433 + PGA1_c13180 0.60 +0.3 1,364,436 - PGA1_c13180 0.60 -2.1 1,364,441 - PGA1_c13180 0.60 +0.6 1,364,441 - PGA1_c13180 0.60 +0.8 1,364,482 - PGA1_c13180 0.64 -0.2 1,364,665 + PGA1_c13180 0.83 +0.1 1,364,681 + PGA1_c13180 0.84 -1.1 1,364,823 + -0.0 1,364,831 - +0.3 1,364,831 - -2.6 1,364,833 + -0.2 1,364,841 - -2.4 1,364,842 - -0.4 1,364,869 + +0.4 1,364,869 + +0.2 1,364,869 + +0.4 1,364,877 - -0.5 1,364,877 - -0.2 1,364,902 - -0.7 1,364,919 - +0.6 1,364,953 - PGA1_c13190 0.12 -0.4 1,364,957 + PGA1_c13190 0.13 +1.1 1,364,957 + PGA1_c13190 0.13 -0.0 1,364,960 - PGA1_c13190 0.13 -0.6 1,364,965 - PGA1_c13190 0.14 -0.2 1,364,970 - PGA1_c13190 0.14 -0.7 1,365,061 - PGA1_c13190 0.25 -1.1 1,365,082 + PGA1_c13190 0.28 +0.6 1,365,083 + PGA1_c13190 0.28 +1.1 1,365,119 + PGA1_c13190 0.32 +0.0 1,365,141 + PGA1_c13190 0.35 +0.1 1,365,145 + PGA1_c13190 0.35 +0.1 1,365,149 + PGA1_c13190 0.36 +0.3 1,365,172 - PGA1_c13190 0.39 -0.7 1,365,199 + PGA1_c13190 0.42 +0.3 1,365,199 + PGA1_c13190 0.42 +1.7 1,365,199 + PGA1_c13190 0.42 +0.5 1,365,207 - PGA1_c13190 0.43 -0.2 1,365,310 + PGA1_c13190 0.55 +0.1 1,365,364 - PGA1_c13190 0.62 +1.6 1,365,551 + PGA1_c13190 0.84 +0.3 1,365,559 - PGA1_c13190 0.85 -1.2 1,365,561 + PGA1_c13190 0.85 -0.6 1,365,569 + PGA1_c13190 0.86 -1.2 1,365,580 + PGA1_c13190 0.88 -2.0 1,365,580 + PGA1_c13190 0.88 -1.4 1,365,590 + PGA1_c13190 0.89 +0.1 1,365,590 + PGA1_c13190 0.89 -1.3 1,365,626 + +0.3 1,365,626 + +1.2 1,365,626 + +1.4 1,365,626 + +0.4 1,365,626 + -0.3 1,365,626 + -0.9 1,365,626 + +1.5 1,365,626 + -0.6 1,365,626 + -1.1 1,365,634 - -0.8 1,365,634 - -0.1 1,365,634 - -0.6 1,365,634 - +0.3 1,365,634 - -0.2 1,365,634 - -1.3 1,365,634 - +0.0 1,365,639 - +0.4 1,365,649 - -0.3 1,365,690 + +0.9 1,365,826 + PGA1_c13200 0.16 -0.5
Or see this region's nucleotide sequence