Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01760 and PGA1_262p01770 are separated by 62 nucleotides PGA1_262p01770 and PGA1_262p01780 are separated by 121 nucleotides PGA1_262p01780 and PGA1_262p01790 are separated by 91 nucleotides
PGA1_262p01760: PGA1_262p01760 - epoxide hydrolase, similar to eukaryotic, at 193,416 to 194,588
_262p01760
PGA1_262p01770: PGA1_262p01770 - putative endoribonuclease, at 194,651 to 194,992
_262p01770
PGA1_262p01780: PGA1_262p01780 - transcriptional regulator, LysR family, at 195,114 to 196,019
_262p01780
PGA1_262p01790: PGA1_262p01790 - aldehyde dehydrogenase, at 196,111 to 197,562
_262p01790
Position (kb) 195
196
197 Strain fitness (log2 ratio) -2
-1
0
1 at 194.164 kb on + strand, within PGA1_262p01760 at 194.172 kb on - strand, within PGA1_262p01760 at 194.302 kb on + strand, within PGA1_262p01760 at 194.321 kb on + strand, within PGA1_262p01760 at 194.403 kb on + strand, within PGA1_262p01760 at 194.414 kb on - strand, within PGA1_262p01760 at 194.446 kb on - strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.459 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.486 kb on + strand at 194.615 kb on + strand at 194.623 kb on - strand at 194.712 kb on - strand, within PGA1_262p01770 at 194.835 kb on + strand, within PGA1_262p01770 at 194.846 kb on + strand, within PGA1_262p01770 at 194.854 kb on - strand, within PGA1_262p01770 at 194.854 kb on - strand, within PGA1_262p01770 at 194.865 kb on - strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.906 kb on - strand, within PGA1_262p01770 at 194.912 kb on + strand, within PGA1_262p01770 at 194.937 kb on + strand, within PGA1_262p01770 at 194.942 kb on - strand, within PGA1_262p01770 at 194.973 kb on + strand at 195.026 kb on - strand at 195.038 kb on + strand at 195.046 kb on + strand at 195.124 kb on + strand at 195.132 kb on - strand at 195.132 kb on - strand at 195.134 kb on + strand at 195.134 kb on + strand at 195.159 kb on + strand at 195.173 kb on + strand at 195.173 kb on + strand at 195.181 kb on - strand at 195.181 kb on - strand at 195.181 kb on - strand at 195.260 kb on + strand, within PGA1_262p01780 at 195.268 kb on - strand, within PGA1_262p01780 at 195.527 kb on - strand, within PGA1_262p01780 at 195.588 kb on + strand, within PGA1_262p01780 at 195.808 kb on + strand, within PGA1_262p01780 at 195.808 kb on + strand, within PGA1_262p01780 at 196.061 kb on + strand at 196.072 kb on + strand at 196.072 kb on + strand at 196.074 kb on + strand at 196.080 kb on - strand at 196.151 kb on - strand at 196.192 kb on - strand at 196.192 kb on - strand at 196.226 kb on + strand at 196.255 kb on - strand at 196.280 kb on - strand, within PGA1_262p01790 at 196.280 kb on - strand, within PGA1_262p01790 at 196.354 kb on - strand, within PGA1_262p01790 at 196.383 kb on + strand, within PGA1_262p01790 at 196.421 kb on + strand, within PGA1_262p01790 at 196.431 kb on + strand, within PGA1_262p01790 at 196.439 kb on - strand, within PGA1_262p01790 at 196.478 kb on - strand, within PGA1_262p01790 at 196.540 kb on + strand, within PGA1_262p01790 at 196.602 kb on + strand, within PGA1_262p01790 at 196.757 kb on + strand, within PGA1_262p01790 at 196.842 kb on + strand, within PGA1_262p01790 at 196.895 kb on + strand, within PGA1_262p01790
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 194,164 + PGA1_262p01760 0.64 -0.8 194,172 - PGA1_262p01760 0.64 +0.7 194,302 + PGA1_262p01760 0.76 +0.0 194,321 + PGA1_262p01760 0.77 -0.7 194,403 + PGA1_262p01760 0.84 +0.5 194,414 - PGA1_262p01760 0.85 -0.2 194,446 - PGA1_262p01760 0.88 +0.3 194,456 + PGA1_262p01760 0.89 -1.5 194,456 + PGA1_262p01760 0.89 +0.5 194,456 + PGA1_262p01760 0.89 -0.5 194,459 - PGA1_262p01760 0.89 -1.7 194,464 - PGA1_262p01760 0.89 -0.5 194,464 - PGA1_262p01760 0.89 -1.4 194,464 - PGA1_262p01760 0.89 +0.3 194,486 + -1.0 194,615 + +0.0 194,623 - -1.3 194,712 - PGA1_262p01770 0.18 +0.6 194,835 + PGA1_262p01770 0.54 -0.2 194,846 + PGA1_262p01770 0.57 -0.1 194,854 - PGA1_262p01770 0.59 +0.5 194,854 - PGA1_262p01770 0.59 +1.0 194,865 - PGA1_262p01770 0.63 +0.4 194,876 + PGA1_262p01770 0.66 +0.2 194,876 + PGA1_262p01770 0.66 -0.8 194,876 + PGA1_262p01770 0.66 +0.3 194,906 - PGA1_262p01770 0.75 -1.9 194,912 + PGA1_262p01770 0.76 -1.5 194,937 + PGA1_262p01770 0.84 -0.7 194,942 - PGA1_262p01770 0.85 +0.2 194,973 + +1.2 195,026 - -0.5 195,038 + -0.8 195,046 + +0.4 195,124 + +0.6 195,132 - +1.1 195,132 - +0.9 195,134 + +0.7 195,134 + +0.6 195,159 + -1.2 195,173 + +0.7 195,173 + +0.0 195,181 - -0.2 195,181 - -1.0 195,181 - +0.0 195,260 + PGA1_262p01780 0.16 +0.3 195,268 - PGA1_262p01780 0.17 +0.7 195,527 - PGA1_262p01780 0.46 +0.9 195,588 + PGA1_262p01780 0.52 -0.9 195,808 + PGA1_262p01780 0.77 +0.8 195,808 + PGA1_262p01780 0.77 -0.3 196,061 + +0.8 196,072 + -1.1 196,072 + +0.5 196,074 + -0.9 196,080 - -2.5 196,151 - +0.2 196,192 - -1.5 196,192 - -0.3 196,226 + -0.1 196,255 - -0.4 196,280 - PGA1_262p01790 0.12 -0.1 196,280 - PGA1_262p01790 0.12 -1.0 196,354 - PGA1_262p01790 0.17 +0.5 196,383 + PGA1_262p01790 0.19 +0.6 196,421 + PGA1_262p01790 0.21 -0.7 196,431 + PGA1_262p01790 0.22 +0.8 196,439 - PGA1_262p01790 0.23 +0.0 196,478 - PGA1_262p01790 0.25 +0.2 196,540 + PGA1_262p01790 0.30 -0.7 196,602 + PGA1_262p01790 0.34 -0.8 196,757 + PGA1_262p01790 0.44 +0.0 196,842 + PGA1_262p01790 0.50 -1.2 196,895 + PGA1_262p01790 0.54 +0.7
Or see this region's nucleotide sequence