Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01320 and PGA1_262p01330 overlap by 14 nucleotides PGA1_262p01330 and PGA1_262p01340 are separated by 81 nucleotides PGA1_262p01340 and PGA1_262p01350 are separated by 80 nucleotides
PGA1_262p01320: PGA1_262p01320 - Uncharacterized protein conserved in bacteria, at 143,235 to 143,936
_262p01320
PGA1_262p01330: PGA1_262p01330 - transcriptional regulator, MarR family, at 143,923 to 144,480
_262p01330
PGA1_262p01340: PGA1_262p01340 - hypothetical protein, at 144,562 to 145,311
_262p01340
PGA1_262p01350: PGA1_262p01350 - putative vanillate O-demethylase oxidoreductase, at 145,392 to 146,336
_262p01350
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1
2 at 143.739 kb on + strand, within PGA1_262p01320 at 143.935 kb on + strand at 144.118 kb on + strand, within PGA1_262p01330 at 144.604 kb on - strand at 144.650 kb on + strand, within PGA1_262p01340 at 144.650 kb on + strand, within PGA1_262p01340 at 144.658 kb on - strand, within PGA1_262p01340 at 144.827 kb on + strand, within PGA1_262p01340 at 144.830 kb on - strand, within PGA1_262p01340 at 144.830 kb on - strand, within PGA1_262p01340 at 144.865 kb on - strand, within PGA1_262p01340 at 144.922 kb on + strand, within PGA1_262p01340 at 145.005 kb on - strand, within PGA1_262p01340 at 145.176 kb on - strand, within PGA1_262p01340 at 145.177 kb on - strand, within PGA1_262p01340 at 145.189 kb on + strand, within PGA1_262p01340 at 145.213 kb on - strand, within PGA1_262p01340 at 145.619 kb on + strand, within PGA1_262p01350
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 143,739 + PGA1_262p01320 0.72 +1.8 143,935 + +0.4 144,118 + PGA1_262p01330 0.35 +0.1 144,604 - +0.4 144,650 + PGA1_262p01340 0.12 +0.7 144,650 + PGA1_262p01340 0.12 +0.5 144,658 - PGA1_262p01340 0.13 +0.3 144,827 + PGA1_262p01340 0.35 +0.1 144,830 - PGA1_262p01340 0.36 -0.4 144,830 - PGA1_262p01340 0.36 +1.0 144,865 - PGA1_262p01340 0.40 +0.6 144,922 + PGA1_262p01340 0.48 +0.0 145,005 - PGA1_262p01340 0.59 +0.5 145,176 - PGA1_262p01340 0.82 +1.0 145,177 - PGA1_262p01340 0.82 -0.1 145,189 + PGA1_262p01340 0.84 -0.6 145,213 - PGA1_262p01340 0.87 +0.6 145,619 + PGA1_262p01350 0.24 +1.3
Or see this region's nucleotide sequence