Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35000 and PGA1_c35010 are separated by 102 nucleotides PGA1_c35010 and PGA1_c35020 are separated by 9 nucleotides PGA1_c35020 and PGA1_c35030 are separated by 154 nucleotides PGA1_c35030 and PGA1_c35040 overlap by 4 nucleotides
PGA1_c35000: PGA1_c35000 - DNA mismatch repair protein MutS, at 3,672,204 to 3,674,837
_c35000
PGA1_c35010: PGA1_c35010 - protein GrpE, at 3,674,940 to 3,675,503
_c35010
PGA1_c35020: PGA1_c35020 - heat-inducible transcription repressor HrcA, at 3,675,513 to 3,676,589
_c35020
PGA1_c35030: PGA1_c35030 - ribonuclease PH, at 3,676,744 to 3,677,457
_c35030
PGA1_c35040: PGA1_c35040 - nucleoside-triphosphatase RdgB, at 3,677,454 to 3,678,068
_c35040
Position (kb)
3675
3676
3677 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3674.519 kb on - strand, within PGA1_c35000 at 3674.558 kb on + strand, within PGA1_c35000 at 3674.558 kb on + strand, within PGA1_c35000 at 3674.701 kb on - strand at 3674.754 kb on + strand at 3674.762 kb on - strand at 3674.862 kb on - strand at 3675.511 kb on - strand at 3675.553 kb on + strand at 3675.594 kb on + strand at 3675.627 kb on - strand, within PGA1_c35020 at 3675.642 kb on + strand, within PGA1_c35020 at 3675.642 kb on + strand, within PGA1_c35020 at 3675.650 kb on - strand, within PGA1_c35020 at 3675.664 kb on - strand, within PGA1_c35020 at 3675.716 kb on + strand, within PGA1_c35020 at 3675.724 kb on - strand, within PGA1_c35020 at 3675.814 kb on + strand, within PGA1_c35020 at 3675.926 kb on - strand, within PGA1_c35020 at 3675.936 kb on - strand, within PGA1_c35020 at 3675.994 kb on + strand, within PGA1_c35020 at 3676.002 kb on - strand, within PGA1_c35020 at 3676.031 kb on + strand, within PGA1_c35020 at 3676.039 kb on - strand, within PGA1_c35020 at 3676.102 kb on + strand, within PGA1_c35020 at 3676.186 kb on - strand, within PGA1_c35020 at 3676.196 kb on + strand, within PGA1_c35020 at 3676.198 kb on + strand, within PGA1_c35020 at 3676.204 kb on - strand, within PGA1_c35020 at 3676.206 kb on + strand, within PGA1_c35020 at 3676.209 kb on - strand, within PGA1_c35020 at 3676.231 kb on + strand, within PGA1_c35020 at 3676.247 kb on - strand, within PGA1_c35020 at 3676.321 kb on + strand, within PGA1_c35020 at 3676.359 kb on - strand, within PGA1_c35020 at 3676.378 kb on + strand, within PGA1_c35020 at 3676.386 kb on - strand, within PGA1_c35020 at 3676.386 kb on - strand, within PGA1_c35020 at 3676.393 kb on + strand, within PGA1_c35020 at 3676.439 kb on + strand, within PGA1_c35020 at 3676.481 kb on - strand, within PGA1_c35020 at 3676.533 kb on + strand at 3676.579 kb on + strand at 3676.625 kb on + strand at 3676.635 kb on - strand at 3676.639 kb on + strand at 3676.639 kb on + strand at 3676.647 kb on - strand at 3676.701 kb on - strand at 3676.712 kb on + strand at 3676.757 kb on + strand at 3676.824 kb on - strand, within PGA1_c35030 at 3676.828 kb on - strand, within PGA1_c35030 at 3676.840 kb on + strand, within PGA1_c35030 at 3676.847 kb on + strand, within PGA1_c35030 at 3676.855 kb on - strand, within PGA1_c35030 at 3676.855 kb on - strand, within PGA1_c35030 at 3676.855 kb on - strand, within PGA1_c35030 at 3676.868 kb on + strand, within PGA1_c35030 at 3676.876 kb on - strand, within PGA1_c35030 at 3676.901 kb on + strand, within PGA1_c35030 at 3676.927 kb on + strand, within PGA1_c35030 at 3677.054 kb on + strand, within PGA1_c35030 at 3677.103 kb on + strand, within PGA1_c35030 at 3677.156 kb on + strand, within PGA1_c35030 at 3677.198 kb on - strand, within PGA1_c35030 at 3677.208 kb on + strand, within PGA1_c35030 at 3677.208 kb on + strand, within PGA1_c35030 at 3677.243 kb on + strand, within PGA1_c35030 at 3677.251 kb on - strand, within PGA1_c35030 at 3677.251 kb on - strand, within PGA1_c35030 at 3677.257 kb on + strand, within PGA1_c35030 at 3677.329 kb on - strand, within PGA1_c35030 at 3677.334 kb on - strand, within PGA1_c35030 at 3677.349 kb on - strand, within PGA1_c35030 at 3677.349 kb on - strand, within PGA1_c35030 at 3677.349 kb on - strand, within PGA1_c35030 at 3677.368 kb on - strand, within PGA1_c35030 at 3677.372 kb on + strand, within PGA1_c35030 at 3677.388 kb on + strand at 3677.436 kb on + strand at 3677.439 kb on + strand at 3677.444 kb on - strand at 3677.496 kb on + strand at 3677.498 kb on + strand at 3677.504 kb on + strand at 3677.578 kb on + strand, within PGA1_c35040
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 3,674,519 - PGA1_c35000 0.88 +0.8 3,674,558 + PGA1_c35000 0.89 -0.9 3,674,558 + PGA1_c35000 0.89 -1.4 3,674,701 - -0.1 3,674,754 + -0.1 3,674,762 - -1.1 3,674,862 - +0.1 3,675,511 - -1.4 3,675,553 + -1.4 3,675,594 + -2.2 3,675,627 - PGA1_c35020 0.11 -1.2 3,675,642 + PGA1_c35020 0.12 +0.3 3,675,642 + PGA1_c35020 0.12 -0.7 3,675,650 - PGA1_c35020 0.13 -2.3 3,675,664 - PGA1_c35020 0.14 -2.2 3,675,716 + PGA1_c35020 0.19 +0.5 3,675,724 - PGA1_c35020 0.20 -1.4 3,675,814 + PGA1_c35020 0.28 -0.6 3,675,926 - PGA1_c35020 0.38 -1.4 3,675,936 - PGA1_c35020 0.39 -1.7 3,675,994 + PGA1_c35020 0.45 -1.0 3,676,002 - PGA1_c35020 0.45 -1.6 3,676,031 + PGA1_c35020 0.48 -3.2 3,676,039 - PGA1_c35020 0.49 -0.2 3,676,102 + PGA1_c35020 0.55 -0.3 3,676,186 - PGA1_c35020 0.62 -1.3 3,676,196 + PGA1_c35020 0.63 -1.5 3,676,198 + PGA1_c35020 0.64 -2.5 3,676,204 - PGA1_c35020 0.64 -0.8 3,676,206 + PGA1_c35020 0.64 -0.1 3,676,209 - PGA1_c35020 0.65 -0.4 3,676,231 + PGA1_c35020 0.67 -0.7 3,676,247 - PGA1_c35020 0.68 -2.0 3,676,321 + PGA1_c35020 0.75 -0.4 3,676,359 - PGA1_c35020 0.79 -1.8 3,676,378 + PGA1_c35020 0.80 -1.9 3,676,386 - PGA1_c35020 0.81 -0.3 3,676,386 - PGA1_c35020 0.81 -0.3 3,676,393 + PGA1_c35020 0.82 -2.1 3,676,439 + PGA1_c35020 0.86 -1.8 3,676,481 - PGA1_c35020 0.90 -1.0 3,676,533 + -1.5 3,676,579 + +0.5 3,676,625 + +1.1 3,676,635 - -1.9 3,676,639 + -2.7 3,676,639 + -0.2 3,676,647 - -0.3 3,676,701 - -0.2 3,676,712 + -0.3 3,676,757 + -1.8 3,676,824 - PGA1_c35030 0.11 +0.1 3,676,828 - PGA1_c35030 0.12 +0.3 3,676,840 + PGA1_c35030 0.13 +0.9 3,676,847 + PGA1_c35030 0.14 +1.0 3,676,855 - PGA1_c35030 0.16 +1.0 3,676,855 - PGA1_c35030 0.16 -0.6 3,676,855 - PGA1_c35030 0.16 -1.7 3,676,868 + PGA1_c35030 0.17 -0.1 3,676,876 - PGA1_c35030 0.18 -2.4 3,676,901 + PGA1_c35030 0.22 +2.3 3,676,927 + PGA1_c35030 0.26 +0.6 3,677,054 + PGA1_c35030 0.43 -0.8 3,677,103 + PGA1_c35030 0.50 -1.0 3,677,156 + PGA1_c35030 0.58 +0.0 3,677,198 - PGA1_c35030 0.64 +0.7 3,677,208 + PGA1_c35030 0.65 -0.4 3,677,208 + PGA1_c35030 0.65 -2.2 3,677,243 + PGA1_c35030 0.70 -1.4 3,677,251 - PGA1_c35030 0.71 +3.1 3,677,251 - PGA1_c35030 0.71 -1.2 3,677,257 + PGA1_c35030 0.72 -1.3 3,677,329 - PGA1_c35030 0.82 +0.8 3,677,334 - PGA1_c35030 0.83 +0.5 3,677,349 - PGA1_c35030 0.85 +0.4 3,677,349 - PGA1_c35030 0.85 -0.2 3,677,349 - PGA1_c35030 0.85 +1.3 3,677,368 - PGA1_c35030 0.87 +0.4 3,677,372 + PGA1_c35030 0.88 -1.1 3,677,388 + -1.7 3,677,436 + +0.8 3,677,439 + -0.4 3,677,444 - -2.3 3,677,496 + -0.1 3,677,498 + +0.0 3,677,504 + -2.0 3,677,578 + PGA1_c35040 0.20 -0.0
Or see this region's nucleotide sequence