Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34740 and PGA1_c34750 are separated by 73 nucleotides PGA1_c34750 and PGA1_c34760 are separated by 105 nucleotides PGA1_c34760 and PGA1_c34770 are separated by 141 nucleotides
PGA1_c34740: PGA1_c34740 - ferrochelatase HemH, at 3,644,319 to 3,645,380
_c34740
PGA1_c34750: PGA1_c34750 - putative aminotransferase class 3, at 3,645,454 to 3,648,483
_c34750
PGA1_c34760: PGA1_c34760 - hypothetical protein, at 3,648,589 to 3,648,771
_c34760
PGA1_c34770: PGA1_c34770 - Uncharacterized protein conserved in bacteria, at 3,648,913 to 3,649,554
_c34770
Position (kb)
3645
3646
3647
3648
3649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3645.401 kb on - strand at 3645.456 kb on - strand at 3645.465 kb on + strand at 3645.486 kb on - strand at 3645.489 kb on - strand at 3645.496 kb on - strand at 3645.501 kb on + strand at 3645.512 kb on - strand at 3645.580 kb on - strand at 3645.587 kb on + strand at 3645.595 kb on - strand at 3645.620 kb on + strand at 3645.763 kb on + strand, within PGA1_c34750 at 3645.765 kb on - strand, within PGA1_c34750 at 3645.770 kb on - strand, within PGA1_c34750 at 3645.776 kb on + strand, within PGA1_c34750 at 3645.831 kb on - strand, within PGA1_c34750 at 3645.831 kb on - strand, within PGA1_c34750 at 3645.892 kb on + strand, within PGA1_c34750 at 3645.906 kb on + strand, within PGA1_c34750 at 3645.925 kb on + strand, within PGA1_c34750 at 3645.951 kb on + strand, within PGA1_c34750 at 3645.996 kb on - strand, within PGA1_c34750 at 3646.160 kb on + strand, within PGA1_c34750 at 3646.241 kb on - strand, within PGA1_c34750 at 3646.248 kb on - strand, within PGA1_c34750 at 3646.258 kb on - strand, within PGA1_c34750 at 3646.282 kb on - strand, within PGA1_c34750 at 3646.304 kb on - strand, within PGA1_c34750 at 3646.352 kb on + strand, within PGA1_c34750 at 3646.352 kb on + strand, within PGA1_c34750 at 3646.352 kb on + strand, within PGA1_c34750 at 3646.357 kb on + strand, within PGA1_c34750 at 3646.360 kb on - strand, within PGA1_c34750 at 3646.365 kb on + strand, within PGA1_c34750 at 3646.375 kb on - strand, within PGA1_c34750 at 3646.426 kb on + strand, within PGA1_c34750 at 3646.436 kb on - strand, within PGA1_c34750 at 3646.454 kb on + strand, within PGA1_c34750 at 3646.574 kb on + strand, within PGA1_c34750 at 3646.620 kb on + strand, within PGA1_c34750 at 3646.694 kb on + strand, within PGA1_c34750 at 3646.699 kb on + strand, within PGA1_c34750 at 3646.699 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.704 kb on + strand, within PGA1_c34750 at 3646.707 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.712 kb on - strand, within PGA1_c34750 at 3646.725 kb on + strand, within PGA1_c34750 at 3646.821 kb on - strand, within PGA1_c34750 at 3646.837 kb on + strand, within PGA1_c34750 at 3646.929 kb on - strand, within PGA1_c34750 at 3646.940 kb on - strand, within PGA1_c34750 at 3646.954 kb on + strand, within PGA1_c34750 at 3647.063 kb on - strand, within PGA1_c34750 at 3647.073 kb on + strand, within PGA1_c34750 at 3647.081 kb on - strand, within PGA1_c34750 at 3647.087 kb on + strand, within PGA1_c34750 at 3647.096 kb on - strand, within PGA1_c34750 at 3647.118 kb on + strand, within PGA1_c34750 at 3647.160 kb on - strand, within PGA1_c34750 at 3647.206 kb on + strand, within PGA1_c34750 at 3647.214 kb on - strand, within PGA1_c34750 at 3647.250 kb on + strand, within PGA1_c34750 at 3647.263 kb on + strand, within PGA1_c34750 at 3647.290 kb on - strand, within PGA1_c34750 at 3647.305 kb on + strand, within PGA1_c34750 at 3647.349 kb on + strand, within PGA1_c34750 at 3647.355 kb on - strand, within PGA1_c34750 at 3647.357 kb on - strand, within PGA1_c34750 at 3647.361 kb on - strand, within PGA1_c34750 at 3647.409 kb on + strand, within PGA1_c34750 at 3647.409 kb on + strand, within PGA1_c34750 at 3647.423 kb on + strand, within PGA1_c34750 at 3647.425 kb on - strand, within PGA1_c34750 at 3647.530 kb on + strand, within PGA1_c34750 at 3647.530 kb on + strand, within PGA1_c34750 at 3647.571 kb on + strand, within PGA1_c34750 at 3647.576 kb on + strand, within PGA1_c34750 at 3647.584 kb on - strand, within PGA1_c34750 at 3647.651 kb on + strand, within PGA1_c34750 at 3647.653 kb on + strand, within PGA1_c34750 at 3647.654 kb on - strand, within PGA1_c34750 at 3647.659 kb on - strand, within PGA1_c34750 at 3647.919 kb on + strand, within PGA1_c34750 at 3647.929 kb on + strand, within PGA1_c34750 at 3647.929 kb on + strand, within PGA1_c34750 at 3647.984 kb on - strand, within PGA1_c34750 at 3647.998 kb on - strand, within PGA1_c34750 at 3648.008 kb on - strand, within PGA1_c34750 at 3648.018 kb on + strand, within PGA1_c34750 at 3648.024 kb on + strand, within PGA1_c34750 at 3648.024 kb on + strand, within PGA1_c34750 at 3648.024 kb on + strand, within PGA1_c34750 at 3648.024 kb on + strand, within PGA1_c34750 at 3648.024 kb on + strand, within PGA1_c34750 at 3648.032 kb on - strand, within PGA1_c34750 at 3648.032 kb on - strand, within PGA1_c34750 at 3648.032 kb on - strand, within PGA1_c34750 at 3648.039 kb on + strand, within PGA1_c34750 at 3648.052 kb on - strand, within PGA1_c34750 at 3648.070 kb on + strand, within PGA1_c34750 at 3648.073 kb on - strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.104 kb on + strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.112 kb on - strand, within PGA1_c34750 at 3648.117 kb on - strand, within PGA1_c34750 at 3648.117 kb on - strand, within PGA1_c34750 at 3648.117 kb on - strand, within PGA1_c34750 at 3648.117 kb on - strand, within PGA1_c34750 at 3648.117 kb on - strand, within PGA1_c34750 at 3648.128 kb on + strand, within PGA1_c34750 at 3648.128 kb on + strand, within PGA1_c34750 at 3648.128 kb on + strand, within PGA1_c34750 at 3648.196 kb on - strand at 3648.254 kb on + strand at 3648.272 kb on + strand at 3648.304 kb on - strand at 3648.304 kb on - strand at 3648.304 kb on - strand at 3648.312 kb on + strand at 3648.469 kb on - strand at 3648.492 kb on + strand at 3648.512 kb on - strand at 3648.924 kb on + strand at 3648.981 kb on + strand, within PGA1_c34770 at 3648.981 kb on + strand, within PGA1_c34770 at 3648.981 kb on + strand, within PGA1_c34770 at 3648.993 kb on + strand, within PGA1_c34770 at 3649.001 kb on - strand, within PGA1_c34770 at 3649.007 kb on + strand, within PGA1_c34770 at 3649.024 kb on + strand, within PGA1_c34770 at 3649.024 kb on + strand, within PGA1_c34770 at 3649.032 kb on - strand, within PGA1_c34770 at 3649.034 kb on - strand, within PGA1_c34770 at 3649.105 kb on + strand, within PGA1_c34770 at 3649.105 kb on + strand, within PGA1_c34770 at 3649.105 kb on + strand, within PGA1_c34770 at 3649.113 kb on - strand, within PGA1_c34770 at 3649.131 kb on - strand, within PGA1_c34770 at 3649.159 kb on + strand, within PGA1_c34770 at 3649.161 kb on - strand, within PGA1_c34770 at 3649.161 kb on - strand, within PGA1_c34770 at 3649.167 kb on - strand, within PGA1_c34770 at 3649.199 kb on + strand, within PGA1_c34770 at 3649.224 kb on + strand, within PGA1_c34770 at 3649.260 kb on + strand, within PGA1_c34770 at 3649.278 kb on + strand, within PGA1_c34770 at 3649.286 kb on - strand, within PGA1_c34770 at 3649.297 kb on + strand, within PGA1_c34770 at 3649.300 kb on - strand, within PGA1_c34770 at 3649.302 kb on + strand, within PGA1_c34770 at 3649.337 kb on + strand, within PGA1_c34770 at 3649.444 kb on - strand, within PGA1_c34770 at 3649.480 kb on - strand, within PGA1_c34770
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 3,645,401 - +0.1 3,645,456 - +0.8 3,645,465 + -0.3 3,645,486 - -0.6 3,645,489 - +0.9 3,645,496 - -0.9 3,645,501 + -0.4 3,645,512 - +0.9 3,645,580 - -0.4 3,645,587 + -0.7 3,645,595 - -2.0 3,645,620 + +0.5 3,645,763 + PGA1_c34750 0.10 +0.2 3,645,765 - PGA1_c34750 0.10 -0.3 3,645,770 - PGA1_c34750 0.10 +1.5 3,645,776 + PGA1_c34750 0.11 +0.3 3,645,831 - PGA1_c34750 0.12 +0.4 3,645,831 - PGA1_c34750 0.12 -1.5 3,645,892 + PGA1_c34750 0.14 +0.8 3,645,906 + PGA1_c34750 0.15 +0.6 3,645,925 + PGA1_c34750 0.16 +1.0 3,645,951 + PGA1_c34750 0.16 +1.2 3,645,996 - PGA1_c34750 0.18 +0.3 3,646,160 + PGA1_c34750 0.23 +0.6 3,646,241 - PGA1_c34750 0.26 +0.1 3,646,248 - PGA1_c34750 0.26 -1.1 3,646,258 - PGA1_c34750 0.27 +0.4 3,646,282 - PGA1_c34750 0.27 -1.1 3,646,304 - PGA1_c34750 0.28 -1.2 3,646,352 + PGA1_c34750 0.30 +1.6 3,646,352 + PGA1_c34750 0.30 -1.2 3,646,352 + PGA1_c34750 0.30 -1.5 3,646,357 + PGA1_c34750 0.30 +1.1 3,646,360 - PGA1_c34750 0.30 +0.3 3,646,365 + PGA1_c34750 0.30 -0.7 3,646,375 - PGA1_c34750 0.30 +1.3 3,646,426 + PGA1_c34750 0.32 -0.2 3,646,436 - PGA1_c34750 0.32 -2.2 3,646,454 + PGA1_c34750 0.33 +0.0 3,646,574 + PGA1_c34750 0.37 -1.9 3,646,620 + PGA1_c34750 0.38 +0.7 3,646,694 + PGA1_c34750 0.41 +0.4 3,646,699 + PGA1_c34750 0.41 +0.4 3,646,699 + PGA1_c34750 0.41 +1.3 3,646,704 + PGA1_c34750 0.41 +0.1 3,646,704 + PGA1_c34750 0.41 -0.4 3,646,704 + PGA1_c34750 0.41 -0.2 3,646,704 + PGA1_c34750 0.41 -0.7 3,646,704 + PGA1_c34750 0.41 +0.8 3,646,704 + PGA1_c34750 0.41 +0.0 3,646,704 + PGA1_c34750 0.41 +0.4 3,646,707 - PGA1_c34750 0.41 +0.7 3,646,712 - PGA1_c34750 0.42 +0.4 3,646,712 - PGA1_c34750 0.42 -0.1 3,646,712 - PGA1_c34750 0.42 +0.7 3,646,712 - PGA1_c34750 0.42 -0.4 3,646,712 - PGA1_c34750 0.42 +1.2 3,646,712 - PGA1_c34750 0.42 +2.0 3,646,725 + PGA1_c34750 0.42 +0.8 3,646,821 - PGA1_c34750 0.45 -2.1 3,646,837 + PGA1_c34750 0.46 +1.8 3,646,929 - PGA1_c34750 0.49 -0.5 3,646,940 - PGA1_c34750 0.49 +0.1 3,646,954 + PGA1_c34750 0.50 -0.6 3,647,063 - PGA1_c34750 0.53 +1.9 3,647,073 + PGA1_c34750 0.53 +0.0 3,647,081 - PGA1_c34750 0.54 -0.4 3,647,087 + PGA1_c34750 0.54 -0.8 3,647,096 - PGA1_c34750 0.54 -0.3 3,647,118 + PGA1_c34750 0.55 +0.1 3,647,160 - PGA1_c34750 0.56 +0.4 3,647,206 + PGA1_c34750 0.58 -0.3 3,647,214 - PGA1_c34750 0.58 -1.0 3,647,250 + PGA1_c34750 0.59 +0.6 3,647,263 + PGA1_c34750 0.60 -0.2 3,647,290 - PGA1_c34750 0.61 -1.7 3,647,305 + PGA1_c34750 0.61 -1.2 3,647,349 + PGA1_c34750 0.63 +0.1 3,647,355 - PGA1_c34750 0.63 +1.3 3,647,357 - PGA1_c34750 0.63 -0.3 3,647,361 - PGA1_c34750 0.63 +1.8 3,647,409 + PGA1_c34750 0.65 -0.8 3,647,409 + PGA1_c34750 0.65 +1.8 3,647,423 + PGA1_c34750 0.65 +0.9 3,647,425 - PGA1_c34750 0.65 +0.7 3,647,530 + PGA1_c34750 0.69 -0.1 3,647,530 + PGA1_c34750 0.69 +0.3 3,647,571 + PGA1_c34750 0.70 +0.7 3,647,576 + PGA1_c34750 0.70 +1.5 3,647,584 - PGA1_c34750 0.70 -0.6 3,647,651 + PGA1_c34750 0.73 -1.3 3,647,653 + PGA1_c34750 0.73 +0.2 3,647,654 - PGA1_c34750 0.73 +0.5 3,647,659 - PGA1_c34750 0.73 +0.9 3,647,919 + PGA1_c34750 0.81 +0.7 3,647,929 + PGA1_c34750 0.82 -0.4 3,647,929 + PGA1_c34750 0.82 +0.2 3,647,984 - PGA1_c34750 0.83 +0.7 3,647,998 - PGA1_c34750 0.84 +0.6 3,648,008 - PGA1_c34750 0.84 -1.6 3,648,018 + PGA1_c34750 0.85 +1.1 3,648,024 + PGA1_c34750 0.85 +0.8 3,648,024 + PGA1_c34750 0.85 -0.4 3,648,024 + PGA1_c34750 0.85 +1.0 3,648,024 + PGA1_c34750 0.85 -0.2 3,648,024 + PGA1_c34750 0.85 -1.2 3,648,032 - PGA1_c34750 0.85 +1.6 3,648,032 - PGA1_c34750 0.85 -1.2 3,648,032 - PGA1_c34750 0.85 -0.9 3,648,039 + PGA1_c34750 0.85 -1.2 3,648,052 - PGA1_c34750 0.86 +1.1 3,648,070 + PGA1_c34750 0.86 -0.2 3,648,073 - PGA1_c34750 0.86 -0.1 3,648,104 + PGA1_c34750 0.87 +0.6 3,648,104 + PGA1_c34750 0.87 +0.4 3,648,104 + PGA1_c34750 0.87 +1.8 3,648,104 + PGA1_c34750 0.87 +1.6 3,648,104 + PGA1_c34750 0.87 +0.5 3,648,104 + PGA1_c34750 0.87 -1.1 3,648,104 + PGA1_c34750 0.87 -0.8 3,648,104 + PGA1_c34750 0.87 -1.3 3,648,104 + PGA1_c34750 0.87 +0.1 3,648,104 + PGA1_c34750 0.87 -0.4 3,648,104 + PGA1_c34750 0.87 +0.9 3,648,104 + PGA1_c34750 0.87 -1.0 3,648,104 + PGA1_c34750 0.87 +2.0 3,648,112 - PGA1_c34750 0.88 -0.2 3,648,112 - PGA1_c34750 0.88 -1.2 3,648,112 - PGA1_c34750 0.88 -0.4 3,648,112 - PGA1_c34750 0.88 -0.2 3,648,112 - PGA1_c34750 0.88 +0.9 3,648,112 - PGA1_c34750 0.88 -0.4 3,648,112 - PGA1_c34750 0.88 -1.2 3,648,112 - PGA1_c34750 0.88 +0.4 3,648,112 - PGA1_c34750 0.88 -0.9 3,648,112 - PGA1_c34750 0.88 +0.7 3,648,112 - PGA1_c34750 0.88 +0.7 3,648,112 - PGA1_c34750 0.88 -0.7 3,648,112 - PGA1_c34750 0.88 -0.3 3,648,112 - PGA1_c34750 0.88 +0.3 3,648,117 - PGA1_c34750 0.88 -0.1 3,648,117 - PGA1_c34750 0.88 +0.5 3,648,117 - PGA1_c34750 0.88 +2.5 3,648,117 - PGA1_c34750 0.88 -1.0 3,648,117 - PGA1_c34750 0.88 +0.8 3,648,128 + PGA1_c34750 0.88 -0.5 3,648,128 + PGA1_c34750 0.88 -1.2 3,648,128 + PGA1_c34750 0.88 -0.0 3,648,196 - +0.5 3,648,254 + -2.5 3,648,272 + -0.7 3,648,304 - +0.1 3,648,304 - +0.4 3,648,304 - +2.7 3,648,312 + +1.1 3,648,469 - +0.6 3,648,492 + +1.6 3,648,512 - -0.3 3,648,924 + -0.1 3,648,981 + PGA1_c34770 0.11 +0.5 3,648,981 + PGA1_c34770 0.11 -3.3 3,648,981 + PGA1_c34770 0.11 -1.0 3,648,993 + PGA1_c34770 0.12 -0.2 3,649,001 - PGA1_c34770 0.14 +0.1 3,649,007 + PGA1_c34770 0.15 +2.1 3,649,024 + PGA1_c34770 0.17 +0.7 3,649,024 + PGA1_c34770 0.17 -0.5 3,649,032 - PGA1_c34770 0.19 +0.5 3,649,034 - PGA1_c34770 0.19 +0.2 3,649,105 + PGA1_c34770 0.30 -0.2 3,649,105 + PGA1_c34770 0.30 -1.2 3,649,105 + PGA1_c34770 0.30 +0.5 3,649,113 - PGA1_c34770 0.31 +0.3 3,649,131 - PGA1_c34770 0.34 +0.1 3,649,159 + PGA1_c34770 0.38 +0.8 3,649,161 - PGA1_c34770 0.39 -0.6 3,649,161 - PGA1_c34770 0.39 -0.8 3,649,167 - PGA1_c34770 0.40 +0.0 3,649,199 + PGA1_c34770 0.45 -0.7 3,649,224 + PGA1_c34770 0.48 +0.8 3,649,260 + PGA1_c34770 0.54 -0.8 3,649,278 + PGA1_c34770 0.57 +1.3 3,649,286 - PGA1_c34770 0.58 +0.5 3,649,297 + PGA1_c34770 0.60 +0.6 3,649,300 - PGA1_c34770 0.60 +0.2 3,649,302 + PGA1_c34770 0.61 +0.1 3,649,337 + PGA1_c34770 0.66 +0.7 3,649,444 - PGA1_c34770 0.83 -0.1 3,649,480 - PGA1_c34770 0.88 -0.5
Or see this region's nucleotide sequence