Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32370 and PGA1_c32390 are separated by 853 nucleotides PGA1_c32390 and PGA1_c32400 are separated by 0 nucleotides PGA1_c32400 and PGA1_c32410 are separated by 69 nucleotides
PGA1_c32370: PGA1_c32370 - 16S ribosomal RNA, at 3,393,228 to 3,394,679
_c32370
PGA1_c32390: PGA1_c32390 - Predicted membrane protein, at 3,395,533 to 3,396,129
_c32390
PGA1_c32400: PGA1_c32400 - putative molybdopterin-binding oxidoreductase, at 3,396,130 to 3,397,050
_c32400
PGA1_c32410: PGA1_c32410 - glutamate synthase-like protein, at 3,397,120 to 3,398,613
_c32410
Position (kb)
3395
3396
3397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3395.348 kb on - strand at 3395.353 kb on - strand at 3395.361 kb on + strand at 3395.384 kb on + strand at 3395.384 kb on + strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.392 kb on - strand at 3395.421 kb on - strand at 3395.531 kb on + strand at 3395.642 kb on - strand, within PGA1_c32390 at 3395.644 kb on + strand, within PGA1_c32390 at 3395.644 kb on + strand, within PGA1_c32390 at 3395.644 kb on + strand, within PGA1_c32390 at 3395.647 kb on - strand, within PGA1_c32390 at 3395.747 kb on - strand, within PGA1_c32390 at 3395.772 kb on + strand, within PGA1_c32390 at 3395.807 kb on + strand, within PGA1_c32390 at 3395.843 kb on + strand, within PGA1_c32390 at 3395.876 kb on - strand, within PGA1_c32390 at 3395.884 kb on - strand, within PGA1_c32390 at 3395.921 kb on - strand, within PGA1_c32390 at 3395.921 kb on - strand, within PGA1_c32390 at 3396.077 kb on + strand at 3396.081 kb on + strand at 3396.091 kb on + strand at 3396.128 kb on + strand at 3396.149 kb on + strand at 3396.155 kb on + strand at 3396.163 kb on - strand at 3396.163 kb on - strand at 3396.163 kb on - strand at 3396.163 kb on - strand at 3396.163 kb on - strand at 3396.204 kb on - strand at 3396.250 kb on + strand, within PGA1_c32400 at 3396.270 kb on + strand, within PGA1_c32400 at 3396.333 kb on - strand, within PGA1_c32400 at 3396.333 kb on - strand, within PGA1_c32400 at 3396.333 kb on - strand, within PGA1_c32400 at 3396.345 kb on + strand, within PGA1_c32400 at 3396.415 kb on + strand, within PGA1_c32400 at 3396.415 kb on + strand, within PGA1_c32400 at 3396.438 kb on - strand, within PGA1_c32400 at 3396.439 kb on + strand, within PGA1_c32400 at 3396.447 kb on - strand, within PGA1_c32400 at 3396.516 kb on + strand, within PGA1_c32400 at 3396.521 kb on + strand, within PGA1_c32400 at 3396.521 kb on + strand, within PGA1_c32400 at 3396.534 kb on + strand, within PGA1_c32400 at 3396.534 kb on - strand, within PGA1_c32400 at 3396.542 kb on - strand, within PGA1_c32400 at 3396.601 kb on + strand, within PGA1_c32400 at 3396.609 kb on - strand, within PGA1_c32400 at 3396.618 kb on + strand, within PGA1_c32400 at 3396.625 kb on - strand, within PGA1_c32400 at 3396.626 kb on - strand, within PGA1_c32400 at 3396.644 kb on - strand, within PGA1_c32400 at 3396.670 kb on + strand, within PGA1_c32400 at 3396.735 kb on + strand, within PGA1_c32400 at 3396.764 kb on - strand, within PGA1_c32400 at 3396.764 kb on - strand, within PGA1_c32400 at 3396.779 kb on + strand, within PGA1_c32400 at 3396.779 kb on + strand, within PGA1_c32400 at 3396.779 kb on + strand, within PGA1_c32400 at 3396.779 kb on + strand, within PGA1_c32400 at 3396.779 kb on + strand, within PGA1_c32400 at 3396.782 kb on - strand, within PGA1_c32400 at 3396.786 kb on + strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.787 kb on - strand, within PGA1_c32400 at 3396.801 kb on + strand, within PGA1_c32400 at 3396.823 kb on + strand, within PGA1_c32400 at 3396.823 kb on + strand, within PGA1_c32400 at 3396.823 kb on + strand, within PGA1_c32400 at 3396.823 kb on + strand, within PGA1_c32400 at 3396.823 kb on + strand, within PGA1_c32400 at 3396.832 kb on - strand, within PGA1_c32400 at 3396.878 kb on - strand, within PGA1_c32400 at 3396.878 kb on - strand, within PGA1_c32400 at 3396.883 kb on - strand, within PGA1_c32400 at 3396.925 kb on + strand, within PGA1_c32400 at 3396.968 kb on + strand at 3397.021 kb on + strand at 3397.026 kb on + strand at 3397.081 kb on + strand at 3397.081 kb on + strand at 3397.089 kb on - strand at 3397.089 kb on - strand at 3397.089 kb on - strand at 3397.089 kb on - strand at 3397.091 kb on + strand at 3397.103 kb on + strand at 3397.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 3,395,348 - +1.2 3,395,353 - -1.8 3,395,361 + +1.6 3,395,384 + -0.4 3,395,384 + +0.8 3,395,392 - -1.0 3,395,392 - +0.4 3,395,392 - +0.1 3,395,392 - +1.1 3,395,392 - +0.1 3,395,392 - +0.9 3,395,392 - +0.3 3,395,392 - +0.6 3,395,392 - +0.8 3,395,392 - +0.3 3,395,392 - -0.9 3,395,392 - +0.0 3,395,421 - +1.3 3,395,531 + -1.0 3,395,642 - PGA1_c32390 0.18 +0.2 3,395,644 + PGA1_c32390 0.19 -0.2 3,395,644 + PGA1_c32390 0.19 +0.2 3,395,644 + PGA1_c32390 0.19 +0.6 3,395,647 - PGA1_c32390 0.19 +0.4 3,395,747 - PGA1_c32390 0.36 -0.4 3,395,772 + PGA1_c32390 0.40 +1.9 3,395,807 + PGA1_c32390 0.46 +0.2 3,395,843 + PGA1_c32390 0.52 +0.5 3,395,876 - PGA1_c32390 0.57 -0.4 3,395,884 - PGA1_c32390 0.59 +0.3 3,395,921 - PGA1_c32390 0.65 +2.1 3,395,921 - PGA1_c32390 0.65 +2.1 3,396,077 + +0.1 3,396,081 + -0.3 3,396,091 + -0.2 3,396,128 + -0.0 3,396,149 + +1.7 3,396,155 + +1.8 3,396,163 - -2.1 3,396,163 - -0.5 3,396,163 - -0.8 3,396,163 - +0.8 3,396,163 - +0.2 3,396,204 - +0.2 3,396,250 + PGA1_c32400 0.13 +0.0 3,396,270 + PGA1_c32400 0.15 -0.7 3,396,333 - PGA1_c32400 0.22 +0.4 3,396,333 - PGA1_c32400 0.22 -1.5 3,396,333 - PGA1_c32400 0.22 -0.1 3,396,345 + PGA1_c32400 0.23 -0.1 3,396,415 + PGA1_c32400 0.31 -0.4 3,396,415 + PGA1_c32400 0.31 -2.0 3,396,438 - PGA1_c32400 0.33 -0.4 3,396,439 + PGA1_c32400 0.34 -0.1 3,396,447 - PGA1_c32400 0.34 -0.5 3,396,516 + PGA1_c32400 0.42 -0.0 3,396,521 + PGA1_c32400 0.42 -0.3 3,396,521 + PGA1_c32400 0.42 +1.6 3,396,534 + PGA1_c32400 0.44 +1.2 3,396,534 - PGA1_c32400 0.44 +0.6 3,396,542 - PGA1_c32400 0.45 +0.5 3,396,601 + PGA1_c32400 0.51 -0.1 3,396,609 - PGA1_c32400 0.52 -1.1 3,396,618 + PGA1_c32400 0.53 +0.9 3,396,625 - PGA1_c32400 0.54 -0.1 3,396,626 - PGA1_c32400 0.54 +1.3 3,396,644 - PGA1_c32400 0.56 -3.2 3,396,670 + PGA1_c32400 0.59 -1.5 3,396,735 + PGA1_c32400 0.66 +2.3 3,396,764 - PGA1_c32400 0.69 -1.8 3,396,764 - PGA1_c32400 0.69 -0.9 3,396,779 + PGA1_c32400 0.70 +1.4 3,396,779 + PGA1_c32400 0.70 +0.9 3,396,779 + PGA1_c32400 0.70 +0.6 3,396,779 + PGA1_c32400 0.70 +0.7 3,396,779 + PGA1_c32400 0.70 +0.5 3,396,782 - PGA1_c32400 0.71 +1.6 3,396,786 + PGA1_c32400 0.71 -1.3 3,396,787 - PGA1_c32400 0.71 -0.4 3,396,787 - PGA1_c32400 0.71 -0.2 3,396,787 - PGA1_c32400 0.71 -0.1 3,396,787 - PGA1_c32400 0.71 +2.9 3,396,787 - PGA1_c32400 0.71 -0.6 3,396,787 - PGA1_c32400 0.71 +1.1 3,396,787 - PGA1_c32400 0.71 +0.5 3,396,801 + PGA1_c32400 0.73 -3.7 3,396,823 + PGA1_c32400 0.75 +1.1 3,396,823 + PGA1_c32400 0.75 -0.4 3,396,823 + PGA1_c32400 0.75 +0.4 3,396,823 + PGA1_c32400 0.75 +1.5 3,396,823 + PGA1_c32400 0.75 +0.6 3,396,832 - PGA1_c32400 0.76 -0.3 3,396,878 - PGA1_c32400 0.81 -0.1 3,396,878 - PGA1_c32400 0.81 -1.8 3,396,883 - PGA1_c32400 0.82 -0.1 3,396,925 + PGA1_c32400 0.86 -0.1 3,396,968 + -1.4 3,397,021 + -1.2 3,397,026 + -0.5 3,397,081 + +0.1 3,397,081 + -2.5 3,397,089 - -1.2 3,397,089 - -0.4 3,397,089 - +0.4 3,397,089 - +2.9 3,397,091 + +0.4 3,397,103 + -0.4 3,397,124 - -1.4
Or see this region's nucleotide sequence