Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02980 and PGA1_c02990 are separated by 217 nucleotides PGA1_c02990 and PGA1_c03000 are separated by 97 nucleotides PGA1_c03000 and PGA1_c03010 are separated by 278 nucleotides PGA1_c03010 and PGA1_c03020 overlap by 1 nucleotides
PGA1_c02980: PGA1_c02980 - glutamate-cysteine ligase, at 286,715 to 288,085
_c02980
PGA1_c02990: PGA1_c02990 - hypothetical protein, at 288,303 to 288,557
_c02990
PGA1_c03000: PGA1_c03000 - YHS domain., at 288,655 to 289,128
_c03000
PGA1_c03010: PGA1_c03010 - glycerol-3-phosphate acyltransferase PlsY, at 289,407 to 290,012
_c03010
PGA1_c03020: PGA1_c03020 - dihydroorotase PyrC, at 290,012 to 291,322
_c03020
Position (kb) 288
289
290 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 288.081 kb on + strand at 288.081 kb on + strand at 288.089 kb on - strand at 288.095 kb on - strand at 288.102 kb on + strand at 288.110 kb on + strand at 288.110 kb on - strand at 288.118 kb on - strand at 288.135 kb on - strand at 288.185 kb on + strand at 288.193 kb on - strand at 288.198 kb on - strand at 288.198 kb on - strand at 288.201 kb on + strand at 288.241 kb on - strand at 288.249 kb on + strand at 288.278 kb on + strand at 288.278 kb on + strand at 288.278 kb on + strand at 288.278 kb on + strand at 288.286 kb on - strand at 288.286 kb on - strand at 288.294 kb on + strand at 288.294 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.295 kb on + strand at 288.300 kb on + strand at 288.303 kb on - strand at 288.303 kb on - strand at 288.303 kb on - strand at 288.303 kb on - strand at 288.303 kb on - strand at 288.357 kb on - strand, within PGA1_c02990 at 288.357 kb on - strand, within PGA1_c02990 at 288.373 kb on - strand, within PGA1_c02990 at 288.380 kb on - strand, within PGA1_c02990 at 288.380 kb on - strand, within PGA1_c02990 at 288.454 kb on + strand, within PGA1_c02990 at 288.454 kb on + strand, within PGA1_c02990 at 288.460 kb on + strand, within PGA1_c02990 at 288.465 kb on + strand, within PGA1_c02990 at 288.480 kb on - strand, within PGA1_c02990 at 288.487 kb on + strand, within PGA1_c02990 at 288.495 kb on - strand, within PGA1_c02990 at 288.526 kb on - strand, within PGA1_c02990 at 288.554 kb on + strand at 288.554 kb on + strand at 288.589 kb on - strand at 288.596 kb on - strand at 288.596 kb on - strand at 288.646 kb on + strand at 288.653 kb on + strand at 288.677 kb on - strand at 288.693 kb on + strand at 288.747 kb on - strand, within PGA1_c03000 at 288.758 kb on - strand, within PGA1_c03000 at 288.768 kb on + strand, within PGA1_c03000 at 288.768 kb on + strand, within PGA1_c03000 at 288.768 kb on + strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.776 kb on - strand, within PGA1_c03000 at 288.831 kb on - strand, within PGA1_c03000 at 288.898 kb on - strand, within PGA1_c03000 at 288.961 kb on + strand, within PGA1_c03000 at 288.976 kb on - strand, within PGA1_c03000 at 289.005 kb on + strand, within PGA1_c03000 at 289.005 kb on + strand, within PGA1_c03000 at 289.013 kb on - strand, within PGA1_c03000 at 289.014 kb on - strand, within PGA1_c03000 at 289.036 kb on - strand, within PGA1_c03000 at 289.065 kb on + strand, within PGA1_c03000 at 289.065 kb on + strand, within PGA1_c03000 at 289.070 kb on + strand, within PGA1_c03000 at 289.073 kb on - strand, within PGA1_c03000 at 289.073 kb on - strand, within PGA1_c03000 at 289.073 kb on - strand, within PGA1_c03000 at 289.088 kb on - strand at 289.097 kb on + strand at 289.130 kb on + strand at 289.131 kb on + strand at 289.138 kb on - strand at 289.139 kb on - strand at 289.158 kb on - strand at 289.169 kb on + strand at 289.169 kb on + strand at 289.194 kb on - strand at 289.233 kb on - strand at 289.243 kb on - strand at 289.278 kb on + strand at 289.333 kb on - strand at 289.352 kb on - strand at 289.413 kb on - strand at 290.017 kb on - strand at 290.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 288,081 + +1.1 288,081 + -0.9 288,089 - -2.0 288,095 - -0.6 288,102 + -0.4 288,110 + -0.3 288,110 - -1.3 288,118 - +0.8 288,135 - -1.4 288,185 + -0.2 288,193 - +0.2 288,198 - +0.7 288,198 - +0.6 288,201 + +0.5 288,241 - -0.4 288,249 + +0.0 288,278 + -0.7 288,278 + -0.7 288,278 + +0.7 288,278 + +0.1 288,286 - +0.2 288,286 - -0.8 288,294 + -0.2 288,294 + +0.7 288,295 + -0.1 288,295 + -0.0 288,295 + -0.7 288,295 + -0.5 288,295 + -1.3 288,295 + +0.9 288,295 + -2.3 288,295 + -0.3 288,295 + +0.3 288,300 + +0.6 288,303 - +0.0 288,303 - -0.2 288,303 - -0.9 288,303 - -1.3 288,303 - +0.7 288,357 - PGA1_c02990 0.21 +0.8 288,357 - PGA1_c02990 0.21 -1.0 288,373 - PGA1_c02990 0.27 +0.6 288,380 - PGA1_c02990 0.30 -0.2 288,380 - PGA1_c02990 0.30 +1.7 288,454 + PGA1_c02990 0.59 -1.1 288,454 + PGA1_c02990 0.59 -3.0 288,460 + PGA1_c02990 0.62 -1.5 288,465 + PGA1_c02990 0.64 +0.9 288,480 - PGA1_c02990 0.69 +0.3 288,487 + PGA1_c02990 0.72 +0.4 288,495 - PGA1_c02990 0.75 -0.2 288,526 - PGA1_c02990 0.87 -0.7 288,554 + -0.3 288,554 + +0.4 288,589 - -0.8 288,596 - +2.1 288,596 - -0.3 288,646 + +0.6 288,653 + +0.2 288,677 - -0.6 288,693 + -2.3 288,747 - PGA1_c03000 0.19 +0.6 288,758 - PGA1_c03000 0.22 -1.2 288,768 + PGA1_c03000 0.24 -0.7 288,768 + PGA1_c03000 0.24 +0.4 288,768 + PGA1_c03000 0.24 +0.2 288,776 - PGA1_c03000 0.26 -0.6 288,776 - PGA1_c03000 0.26 -0.1 288,776 - PGA1_c03000 0.26 -0.0 288,776 - PGA1_c03000 0.26 -1.1 288,776 - PGA1_c03000 0.26 -0.0 288,776 - PGA1_c03000 0.26 -0.3 288,831 - PGA1_c03000 0.37 -1.0 288,898 - PGA1_c03000 0.51 -0.1 288,961 + PGA1_c03000 0.65 +0.7 288,976 - PGA1_c03000 0.68 -1.2 289,005 + PGA1_c03000 0.74 -1.9 289,005 + PGA1_c03000 0.74 +0.6 289,013 - PGA1_c03000 0.76 -0.6 289,014 - PGA1_c03000 0.76 -2.0 289,036 - PGA1_c03000 0.80 +0.2 289,065 + PGA1_c03000 0.86 -1.2 289,065 + PGA1_c03000 0.86 +0.4 289,070 + PGA1_c03000 0.88 +0.6 289,073 - PGA1_c03000 0.88 +0.1 289,073 - PGA1_c03000 0.88 +0.1 289,073 - PGA1_c03000 0.88 -0.6 289,088 - -1.3 289,097 + +0.9 289,130 + -0.9 289,131 + -0.2 289,138 - +0.4 289,139 - +0.9 289,158 - -0.8 289,169 + -1.6 289,169 + -0.0 289,194 - +0.5 289,233 - -0.9 289,243 - -0.1 289,278 + -0.8 289,333 - -0.5 289,352 - +0.1 289,413 - +0.2 290,017 - -0.2 290,065 - -0.7
Or see this region's nucleotide sequence