Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c25130 and PGA1_c25140 are separated by 65 nucleotides PGA1_c25140 and PGA1_c25150 are separated by 182 nucleotides
PGA1_c25130: PGA1_c25130 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 2,616,338 to 2,617,084
_c25130
PGA1_c25140: PGA1_c25140 - hydroxyacylglutathione hydrolase GloB, at 2,617,150 to 2,617,923
_c25140
PGA1_c25150: PGA1_c25150 - ATP-dependent Clp protease ATP-binding subunit, at 2,618,106 to 2,620,427
_c25150
Position (kb)
2617
2618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2616.152 kb on + strand at 2616.199 kb on - strand at 2616.200 kb on - strand at 2616.221 kb on + strand at 2616.235 kb on + strand at 2616.235 kb on + strand at 2616.235 kb on + strand at 2616.235 kb on + strand at 2616.240 kb on + strand at 2616.240 kb on + strand at 2616.243 kb on - strand at 2616.243 kb on - strand at 2616.248 kb on - strand at 2616.248 kb on - strand at 2616.264 kb on - strand at 2616.271 kb on - strand at 2616.271 kb on - strand at 2616.331 kb on + strand at 2616.343 kb on - strand at 2616.349 kb on - strand at 2616.365 kb on - strand at 2616.410 kb on + strand at 2616.434 kb on - strand, within PGA1_c25130 at 2616.524 kb on - strand, within PGA1_c25130 at 2616.627 kb on - strand, within PGA1_c25130 at 2616.629 kb on - strand, within PGA1_c25130 at 2616.629 kb on - strand, within PGA1_c25130 at 2616.639 kb on - strand, within PGA1_c25130 at 2616.754 kb on + strand, within PGA1_c25130 at 2616.754 kb on + strand, within PGA1_c25130 at 2616.866 kb on + strand, within PGA1_c25130 at 2616.932 kb on + strand, within PGA1_c25130 at 2616.972 kb on - strand, within PGA1_c25130 at 2616.973 kb on - strand, within PGA1_c25130 at 2616.973 kb on - strand, within PGA1_c25130 at 2617.031 kb on + strand at 2617.177 kb on - strand at 2617.235 kb on - strand, within PGA1_c25140 at 2617.300 kb on - strand, within PGA1_c25140 at 2617.319 kb on + strand, within PGA1_c25140 at 2617.321 kb on + strand, within PGA1_c25140 at 2617.329 kb on - strand, within PGA1_c25140 at 2617.380 kb on + strand, within PGA1_c25140 at 2617.380 kb on + strand, within PGA1_c25140 at 2617.380 kb on + strand, within PGA1_c25140 at 2617.388 kb on - strand, within PGA1_c25140 at 2617.388 kb on - strand, within PGA1_c25140 at 2617.388 kb on - strand, within PGA1_c25140 at 2617.388 kb on - strand, within PGA1_c25140 at 2617.388 kb on - strand, within PGA1_c25140 at 2617.455 kb on - strand, within PGA1_c25140 at 2617.465 kb on - strand, within PGA1_c25140 at 2617.534 kb on + strand, within PGA1_c25140 at 2617.540 kb on + strand, within PGA1_c25140 at 2617.542 kb on + strand, within PGA1_c25140 at 2617.564 kb on + strand, within PGA1_c25140 at 2617.564 kb on - strand, within PGA1_c25140 at 2617.569 kb on + strand, within PGA1_c25140 at 2617.569 kb on + strand, within PGA1_c25140 at 2617.598 kb on - strand, within PGA1_c25140 at 2617.600 kb on + strand, within PGA1_c25140 at 2617.600 kb on + strand, within PGA1_c25140 at 2617.608 kb on + strand, within PGA1_c25140 at 2617.626 kb on + strand, within PGA1_c25140 at 2617.635 kb on - strand, within PGA1_c25140 at 2617.713 kb on + strand, within PGA1_c25140 at 2617.713 kb on + strand, within PGA1_c25140 at 2617.713 kb on + strand, within PGA1_c25140 at 2617.726 kb on + strand, within PGA1_c25140 at 2617.750 kb on + strand, within PGA1_c25140 at 2617.769 kb on - strand, within PGA1_c25140 at 2617.769 kb on - strand, within PGA1_c25140 at 2617.817 kb on - strand, within PGA1_c25140 at 2617.817 kb on - strand, within PGA1_c25140 at 2617.817 kb on - strand, within PGA1_c25140 at 2617.822 kb on - strand, within PGA1_c25140 at 2617.849 kb on - strand at 2617.866 kb on - strand at 2617.877 kb on + strand at 2617.912 kb on + strand at 2618.033 kb on + strand at 2618.036 kb on - strand at 2618.041 kb on - strand at 2618.041 kb on - strand at 2618.043 kb on + strand at 2618.043 kb on + strand at 2618.051 kb on - strand at 2618.051 kb on - strand at 2618.051 kb on - strand at 2618.051 kb on - strand at 2618.051 kb on - strand at 2618.104 kb on - strand at 2618.125 kb on + strand at 2618.133 kb on - strand at 2618.133 kb on - strand at 2618.196 kb on - strand at 2618.253 kb on + strand at 2618.346 kb on - strand, within PGA1_c25150 at 2618.401 kb on + strand, within PGA1_c25150 at 2618.410 kb on + strand, within PGA1_c25150 at 2618.434 kb on - strand, within PGA1_c25150 at 2618.447 kb on - strand, within PGA1_c25150 at 2618.480 kb on - strand, within PGA1_c25150 at 2618.588 kb on + strand, within PGA1_c25150 at 2618.589 kb on - strand, within PGA1_c25150 at 2618.596 kb on - strand, within PGA1_c25150 at 2618.606 kb on + strand, within PGA1_c25150 at 2618.606 kb on + strand, within PGA1_c25150 at 2618.728 kb on + strand, within PGA1_c25150 at 2618.739 kb on - strand, within PGA1_c25150
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 2,616,152 + +1.3 2,616,199 - -1.7 2,616,200 - -1.0 2,616,221 + -0.4 2,616,235 + -0.0 2,616,235 + -0.8 2,616,235 + +0.3 2,616,235 + +0.2 2,616,240 + -0.7 2,616,240 + -0.1 2,616,243 - +0.1 2,616,243 - +1.1 2,616,248 - -0.7 2,616,248 - -0.7 2,616,264 - +1.5 2,616,271 - +0.1 2,616,271 - -1.0 2,616,331 + +0.5 2,616,343 - -1.8 2,616,349 - -0.4 2,616,365 - +0.5 2,616,410 + -2.4 2,616,434 - PGA1_c25130 0.13 -1.3 2,616,524 - PGA1_c25130 0.25 -1.8 2,616,627 - PGA1_c25130 0.39 -0.5 2,616,629 - PGA1_c25130 0.39 -1.3 2,616,629 - PGA1_c25130 0.39 -0.9 2,616,639 - PGA1_c25130 0.40 -2.5 2,616,754 + PGA1_c25130 0.56 -1.3 2,616,754 + PGA1_c25130 0.56 -2.0 2,616,866 + PGA1_c25130 0.71 -1.3 2,616,932 + PGA1_c25130 0.80 -0.5 2,616,972 - PGA1_c25130 0.85 -1.8 2,616,973 - PGA1_c25130 0.85 -1.8 2,616,973 - PGA1_c25130 0.85 -1.2 2,617,031 + -1.2 2,617,177 - +0.9 2,617,235 - PGA1_c25140 0.11 +1.1 2,617,300 - PGA1_c25140 0.19 +1.0 2,617,319 + PGA1_c25140 0.22 +1.7 2,617,321 + PGA1_c25140 0.22 +0.2 2,617,329 - PGA1_c25140 0.23 +1.2 2,617,380 + PGA1_c25140 0.30 +0.7 2,617,380 + PGA1_c25140 0.30 -3.0 2,617,380 + PGA1_c25140 0.30 +0.5 2,617,388 - PGA1_c25140 0.31 +0.5 2,617,388 - PGA1_c25140 0.31 +1.5 2,617,388 - PGA1_c25140 0.31 -0.2 2,617,388 - PGA1_c25140 0.31 +0.4 2,617,388 - PGA1_c25140 0.31 -0.2 2,617,455 - PGA1_c25140 0.39 +0.0 2,617,465 - PGA1_c25140 0.41 -0.3 2,617,534 + PGA1_c25140 0.50 +1.3 2,617,540 + PGA1_c25140 0.50 +0.9 2,617,542 + PGA1_c25140 0.51 +1.2 2,617,564 + PGA1_c25140 0.53 -0.1 2,617,564 - PGA1_c25140 0.53 -0.8 2,617,569 + PGA1_c25140 0.54 -0.3 2,617,569 + PGA1_c25140 0.54 -1.0 2,617,598 - PGA1_c25140 0.58 +0.6 2,617,600 + PGA1_c25140 0.58 +0.7 2,617,600 + PGA1_c25140 0.58 +2.2 2,617,608 + PGA1_c25140 0.59 +0.1 2,617,626 + PGA1_c25140 0.61 -0.2 2,617,635 - PGA1_c25140 0.63 +0.6 2,617,713 + PGA1_c25140 0.73 -0.3 2,617,713 + PGA1_c25140 0.73 -0.7 2,617,713 + PGA1_c25140 0.73 +1.1 2,617,726 + PGA1_c25140 0.74 +0.0 2,617,750 + PGA1_c25140 0.78 +0.3 2,617,769 - PGA1_c25140 0.80 +0.0 2,617,769 - PGA1_c25140 0.80 +0.3 2,617,817 - PGA1_c25140 0.86 -0.6 2,617,817 - PGA1_c25140 0.86 -0.4 2,617,817 - PGA1_c25140 0.86 -1.5 2,617,822 - PGA1_c25140 0.87 +0.0 2,617,849 - -1.4 2,617,866 - -1.4 2,617,877 + -0.4 2,617,912 + -1.6 2,618,033 + -0.3 2,618,036 - -0.6 2,618,041 - -0.7 2,618,041 - +1.0 2,618,043 + +1.5 2,618,043 + -0.4 2,618,051 - -2.8 2,618,051 - -0.6 2,618,051 - +0.6 2,618,051 - -1.1 2,618,051 - -1.6 2,618,104 - +0.5 2,618,125 + -0.6 2,618,133 - -1.1 2,618,133 - -0.2 2,618,196 - +1.2 2,618,253 + -1.4 2,618,346 - PGA1_c25150 0.10 -0.1 2,618,401 + PGA1_c25150 0.13 -0.7 2,618,410 + PGA1_c25150 0.13 -1.8 2,618,434 - PGA1_c25150 0.14 -1.0 2,618,447 - PGA1_c25150 0.15 -0.3 2,618,480 - PGA1_c25150 0.16 +0.1 2,618,588 + PGA1_c25150 0.21 -1.8 2,618,589 - PGA1_c25150 0.21 -0.9 2,618,596 - PGA1_c25150 0.21 -0.3 2,618,606 + PGA1_c25150 0.22 -0.6 2,618,606 + PGA1_c25150 0.22 -0.6 2,618,728 + PGA1_c25150 0.27 -1.1 2,618,739 - PGA1_c25150 0.27 -0.1
Or see this region's nucleotide sequence