Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22090 and PGA1_c22100 are separated by 28 nucleotides PGA1_c22100 and PGA1_c22110 are separated by 456 nucleotides PGA1_c22110 and PGA1_c22120 are separated by 14 nucleotides PGA1_c22120 and PGA1_c22130 overlap by 4 nucleotides
PGA1_c22090: PGA1_c22090 - nitroreductase-like protein, at 2,289,724 to 2,290,395
_c22090
PGA1_c22100: PGA1_c22100 - D-tyrosyl-tRNA(Tyr) deacylase Dtd, at 2,290,424 to 2,290,870
_c22100
PGA1_c22110: PGA1_c22110 - putative glutathione-binding protein GsiB, at 2,291,327 to 2,292,913
_c22110
PGA1_c22120: PGA1_c22120 - glutathione transport system permease protein GsiC, at 2,292,928 to 2,293,884
_c22120
PGA1_c22130: PGA1_c22130 - putative glutathione transport system permease protein GsiD, at 2,293,881 to 2,294,696
_c22130
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2290.344 kb on + strand at 2290.344 kb on + strand at 2290.372 kb on - strand at 2290.393 kb on - strand at 2290.410 kb on + strand at 2290.439 kb on - strand at 2290.439 kb on - strand at 2290.521 kb on - strand, within PGA1_c22100 at 2290.526 kb on - strand, within PGA1_c22100 at 2290.558 kb on - strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.716 kb on + strand, within PGA1_c22100 at 2290.721 kb on + strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.724 kb on - strand, within PGA1_c22100 at 2290.729 kb on - strand, within PGA1_c22100 at 2290.740 kb on + strand, within PGA1_c22100 at 2290.767 kb on + strand, within PGA1_c22100 at 2290.817 kb on + strand, within PGA1_c22100 at 2290.865 kb on - strand at 2290.905 kb on + strand at 2290.913 kb on - strand at 2290.913 kb on - strand at 2291.002 kb on + strand at 2291.043 kb on + strand at 2291.050 kb on + strand at 2291.051 kb on - strand at 2291.139 kb on + strand at 2291.199 kb on - strand at 2291.241 kb on - strand at 2291.260 kb on - strand at 2291.301 kb on - strand at 2291.314 kb on - strand at 2291.314 kb on - strand at 2291.413 kb on + strand at 2291.429 kb on + strand at 2291.437 kb on - strand at 2291.437 kb on - strand at 2291.437 kb on - strand at 2291.464 kb on - strand at 2291.582 kb on + strand, within PGA1_c22110 at 2291.619 kb on - strand, within PGA1_c22110 at 2291.665 kb on + strand, within PGA1_c22110 at 2291.665 kb on + strand, within PGA1_c22110 at 2291.716 kb on + strand, within PGA1_c22110 at 2291.740 kb on - strand, within PGA1_c22110 at 2291.809 kb on + strand, within PGA1_c22110 at 2291.815 kb on + strand, within PGA1_c22110 at 2291.835 kb on + strand, within PGA1_c22110 at 2292.021 kb on - strand, within PGA1_c22110 at 2292.108 kb on - strand, within PGA1_c22110 at 2292.130 kb on + strand, within PGA1_c22110 at 2292.148 kb on - strand, within PGA1_c22110 at 2292.378 kb on + strand, within PGA1_c22110 at 2292.481 kb on - strand, within PGA1_c22110 at 2292.491 kb on - strand, within PGA1_c22110 at 2292.569 kb on - strand, within PGA1_c22110 at 2292.603 kb on + strand, within PGA1_c22110 at 2292.623 kb on + strand, within PGA1_c22110 at 2292.623 kb on + strand, within PGA1_c22110 at 2292.667 kb on - strand, within PGA1_c22110 at 2292.730 kb on - strand, within PGA1_c22110 at 2292.756 kb on + strand at 2292.788 kb on + strand at 2292.796 kb on - strand at 2292.839 kb on + strand at 2292.839 kb on + strand at 2292.844 kb on + strand at 2292.918 kb on + strand at 2292.918 kb on + strand at 2292.926 kb on - strand at 2292.954 kb on - strand at 2292.957 kb on + strand at 2292.971 kb on + strand at 2292.979 kb on - strand at 2293.026 kb on + strand, within PGA1_c22120 at 2293.042 kb on - strand, within PGA1_c22120 at 2293.067 kb on + strand, within PGA1_c22120 at 2293.067 kb on + strand, within PGA1_c22120 at 2293.075 kb on - strand, within PGA1_c22120 at 2293.075 kb on - strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.186 kb on + strand, within PGA1_c22120 at 2293.194 kb on - strand, within PGA1_c22120 at 2293.287 kb on + strand, within PGA1_c22120 at 2293.329 kb on + strand, within PGA1_c22120 at 2293.336 kb on + strand, within PGA1_c22120 at 2293.336 kb on + strand, within PGA1_c22120 at 2293.350 kb on - strand, within PGA1_c22120 at 2293.392 kb on - strand, within PGA1_c22120 at 2293.422 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.503 kb on - strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.548 kb on + strand, within PGA1_c22120 at 2293.604 kb on + strand, within PGA1_c22120 at 2293.604 kb on + strand, within PGA1_c22120 at 2293.612 kb on - strand, within PGA1_c22120 at 2293.632 kb on + strand, within PGA1_c22120 at 2293.632 kb on + strand, within PGA1_c22120 at 2293.635 kb on - strand, within PGA1_c22120 at 2293.682 kb on + strand, within PGA1_c22120 at 2293.690 kb on - strand, within PGA1_c22120 at 2293.690 kb on - strand, within PGA1_c22120 at 2293.694 kb on + strand, within PGA1_c22120 at 2293.737 kb on + strand, within PGA1_c22120 at 2293.740 kb on + strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.788 kb on - strand, within PGA1_c22120 at 2293.793 kb on - strand at 2293.809 kb on + strand at 2293.864 kb on + strand at 2293.864 kb on + strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.872 kb on - strand at 2293.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 2,290,344 + -0.6 2,290,344 + -0.9 2,290,372 - +0.6 2,290,393 - +0.4 2,290,410 + -1.0 2,290,439 - +0.8 2,290,439 - -0.6 2,290,521 - PGA1_c22100 0.22 +1.0 2,290,526 - PGA1_c22100 0.23 -0.6 2,290,558 - PGA1_c22100 0.30 -0.6 2,290,716 + PGA1_c22100 0.65 -0.8 2,290,716 + PGA1_c22100 0.65 -1.1 2,290,716 + PGA1_c22100 0.65 -1.3 2,290,716 + PGA1_c22100 0.65 +0.2 2,290,721 + PGA1_c22100 0.66 +1.3 2,290,724 - PGA1_c22100 0.67 -0.1 2,290,724 - PGA1_c22100 0.67 -0.5 2,290,724 - PGA1_c22100 0.67 +0.1 2,290,729 - PGA1_c22100 0.68 -0.4 2,290,740 + PGA1_c22100 0.71 -0.7 2,290,767 + PGA1_c22100 0.77 +0.1 2,290,817 + PGA1_c22100 0.88 +0.5 2,290,865 - -0.8 2,290,905 + -1.3 2,290,913 - -1.4 2,290,913 - +0.0 2,291,002 + -1.0 2,291,043 + +0.4 2,291,050 + -1.4 2,291,051 - +0.6 2,291,139 + +1.0 2,291,199 - +0.3 2,291,241 - +0.6 2,291,260 - +1.0 2,291,301 - +1.4 2,291,314 - -0.4 2,291,314 - -0.3 2,291,413 + +0.7 2,291,429 + +2.0 2,291,437 - -1.2 2,291,437 - +0.7 2,291,437 - -0.3 2,291,464 - -0.0 2,291,582 + PGA1_c22110 0.16 +0.7 2,291,619 - PGA1_c22110 0.18 -0.2 2,291,665 + PGA1_c22110 0.21 +1.3 2,291,665 + PGA1_c22110 0.21 +1.3 2,291,716 + PGA1_c22110 0.25 +1.1 2,291,740 - PGA1_c22110 0.26 +0.1 2,291,809 + PGA1_c22110 0.30 +2.1 2,291,815 + PGA1_c22110 0.31 +0.7 2,291,835 + PGA1_c22110 0.32 -0.2 2,292,021 - PGA1_c22110 0.44 -0.0 2,292,108 - PGA1_c22110 0.49 +1.3 2,292,130 + PGA1_c22110 0.51 -0.3 2,292,148 - PGA1_c22110 0.52 +0.2 2,292,378 + PGA1_c22110 0.66 +0.8 2,292,481 - PGA1_c22110 0.73 +0.5 2,292,491 - PGA1_c22110 0.73 -0.6 2,292,569 - PGA1_c22110 0.78 +0.1 2,292,603 + PGA1_c22110 0.80 -0.2 2,292,623 + PGA1_c22110 0.82 +1.5 2,292,623 + PGA1_c22110 0.82 +1.1 2,292,667 - PGA1_c22110 0.84 +1.0 2,292,730 - PGA1_c22110 0.88 +1.2 2,292,756 + +1.9 2,292,788 + +0.4 2,292,796 - +1.3 2,292,839 + -0.3 2,292,839 + -0.2 2,292,844 + +3.1 2,292,918 + +1.0 2,292,918 + +2.9 2,292,926 - +0.8 2,292,954 - +0.4 2,292,957 + +1.1 2,292,971 + +0.4 2,292,979 - +0.7 2,293,026 + PGA1_c22120 0.10 -0.2 2,293,042 - PGA1_c22120 0.12 +1.6 2,293,067 + PGA1_c22120 0.15 +1.4 2,293,067 + PGA1_c22120 0.15 -0.2 2,293,075 - PGA1_c22120 0.15 +2.4 2,293,075 - PGA1_c22120 0.15 -0.8 2,293,186 + PGA1_c22120 0.27 +0.2 2,293,186 + PGA1_c22120 0.27 +1.8 2,293,186 + PGA1_c22120 0.27 +1.6 2,293,194 - PGA1_c22120 0.28 +0.1 2,293,287 + PGA1_c22120 0.38 -0.1 2,293,329 + PGA1_c22120 0.42 -0.2 2,293,336 + PGA1_c22120 0.43 +1.1 2,293,336 + PGA1_c22120 0.43 +0.1 2,293,350 - PGA1_c22120 0.44 +0.5 2,293,392 - PGA1_c22120 0.48 +0.3 2,293,422 - PGA1_c22120 0.52 +0.4 2,293,503 - PGA1_c22120 0.60 +0.3 2,293,503 - PGA1_c22120 0.60 +0.6 2,293,503 - PGA1_c22120 0.60 +0.1 2,293,548 + PGA1_c22120 0.65 +0.7 2,293,548 + PGA1_c22120 0.65 +1.2 2,293,548 + PGA1_c22120 0.65 +1.4 2,293,604 + PGA1_c22120 0.71 +0.5 2,293,604 + PGA1_c22120 0.71 +0.3 2,293,612 - PGA1_c22120 0.71 +2.2 2,293,632 + PGA1_c22120 0.74 +1.4 2,293,632 + PGA1_c22120 0.74 +1.2 2,293,635 - PGA1_c22120 0.74 +0.4 2,293,682 + PGA1_c22120 0.79 +1.5 2,293,690 - PGA1_c22120 0.80 +0.3 2,293,690 - PGA1_c22120 0.80 +0.8 2,293,694 + PGA1_c22120 0.80 +0.3 2,293,737 + PGA1_c22120 0.85 +0.7 2,293,740 + PGA1_c22120 0.85 +0.9 2,293,788 - PGA1_c22120 0.90 +0.4 2,293,788 - PGA1_c22120 0.90 +1.9 2,293,788 - PGA1_c22120 0.90 +1.1 2,293,793 - +1.8 2,293,809 + +1.5 2,293,864 + +0.1 2,293,864 + +1.0 2,293,872 - +1.4 2,293,872 - +2.3 2,293,872 - +1.1 2,293,872 - -0.3 2,293,898 - -0.2
Or see this region's nucleotide sequence