Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c18500 and PGA1_c18510 are separated by 3 nucleotides PGA1_c18510 and PGA1_c18520 are separated by 9 nucleotides PGA1_c18520 and PGA1_c18530 are separated by 5 nucleotides PGA1_c18530 and PGA1_c18540 overlap by 1 nucleotides
PGA1_c18500: PGA1_c18500 - hypothetical protein, at 1,929,731 to 1,930,297
_c18500
PGA1_c18510: PGA1_c18510 - Protein of unknown function (DUF1064)., at 1,930,301 to 1,930,717
_c18510
PGA1_c18520: PGA1_c18520 - Uncharacterized protein conserved in bacteria, at 1,930,727 to 1,930,987
_c18520
PGA1_c18530: PGA1_c18530 - hypothetical protein, at 1,930,993 to 1,931,301
_c18530
PGA1_c18540: PGA1_c18540 - hypothetical protein, at 1,931,301 to 1,932,248
_c18540
Position (kb)
1930
1931 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1929.728 kb on + strand at 1929.755 kb on - strand at 1929.763 kb on + strand at 1929.763 kb on + strand at 1929.764 kb on + strand at 1929.772 kb on - strand at 1929.777 kb on + strand at 1929.783 kb on + strand at 1929.783 kb on + strand at 1929.783 kb on + strand at 1929.791 kb on - strand, within PGA1_c18500 at 1929.791 kb on - strand, within PGA1_c18500 at 1929.791 kb on - strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.796 kb on - strand, within PGA1_c18500 at 1929.802 kb on + strand, within PGA1_c18500 at 1929.802 kb on + strand, within PGA1_c18500 at 1929.810 kb on - strand, within PGA1_c18500 at 1929.825 kb on + strand, within PGA1_c18500 at 1929.865 kb on + strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.896 kb on - strand, within PGA1_c18500 at 1929.898 kb on + strand, within PGA1_c18500 at 1929.909 kb on + strand, within PGA1_c18500 at 1929.909 kb on + strand, within PGA1_c18500 at 1929.940 kb on - strand, within PGA1_c18500 at 1929.942 kb on - strand, within PGA1_c18500 at 1930.041 kb on + strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.098 kb on + strand, within PGA1_c18500 at 1930.098 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.110 kb on + strand, within PGA1_c18500 at 1930.124 kb on - strand, within PGA1_c18500 at 1930.161 kb on + strand, within PGA1_c18500 at 1930.161 kb on + strand, within PGA1_c18500 at 1930.169 kb on - strand, within PGA1_c18500 at 1930.173 kb on + strand, within PGA1_c18500 at 1930.174 kb on - strand, within PGA1_c18500 at 1930.210 kb on - strand, within PGA1_c18500 at 1930.211 kb on - strand, within PGA1_c18500 at 1930.225 kb on - strand, within PGA1_c18500 at 1930.230 kb on - strand, within PGA1_c18500 at 1930.267 kb on + strand at 1930.268 kb on - strand at 1930.272 kb on + strand at 1930.275 kb on - strand at 1930.275 kb on - strand at 1930.280 kb on - strand at 1930.334 kb on + strand at 1930.334 kb on + strand at 1930.342 kb on - strand at 1930.362 kb on + strand, within PGA1_c18510 at 1930.386 kb on + strand, within PGA1_c18510 at 1930.488 kb on - strand, within PGA1_c18510 at 1930.508 kb on + strand, within PGA1_c18510 at 1930.535 kb on + strand, within PGA1_c18510 at 1930.567 kb on + strand, within PGA1_c18510 at 1930.567 kb on + strand, within PGA1_c18510 at 1930.575 kb on - strand, within PGA1_c18510 at 1930.582 kb on + strand, within PGA1_c18510 at 1930.652 kb on + strand, within PGA1_c18510 at 1930.658 kb on + strand, within PGA1_c18510 at 1930.663 kb on + strand, within PGA1_c18510 at 1930.674 kb on - strand, within PGA1_c18510 at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.694 kb on - strand at 1930.701 kb on + strand at 1930.718 kb on - strand at 1930.724 kb on + strand at 1930.732 kb on - strand at 1930.738 kb on - strand at 1930.828 kb on + strand, within PGA1_c18520 at 1930.844 kb on - strand, within PGA1_c18520 at 1930.860 kb on + strand, within PGA1_c18520 at 1930.860 kb on + strand, within PGA1_c18520 at 1930.902 kb on + strand, within PGA1_c18520 at 1930.910 kb on - strand, within PGA1_c18520 at 1930.911 kb on + strand, within PGA1_c18520 at 1930.920 kb on - strand, within PGA1_c18520 at 1930.941 kb on - strand, within PGA1_c18520 at 1930.962 kb on - strand at 1930.996 kb on + strand at 1930.996 kb on - strand at 1931.036 kb on - strand, within PGA1_c18530 at 1931.056 kb on + strand, within PGA1_c18530 at 1931.064 kb on - strand, within PGA1_c18530 at 1931.148 kb on + strand, within PGA1_c18530 at 1931.158 kb on + strand, within PGA1_c18530 at 1931.188 kb on + strand, within PGA1_c18530 at 1931.200 kb on - strand, within PGA1_c18530 at 1931.232 kb on - strand, within PGA1_c18530 at 1931.311 kb on + strand at 1931.319 kb on - strand at 1931.319 kb on - strand at 1931.378 kb on + strand at 1931.383 kb on + strand at 1931.409 kb on - strand, within PGA1_c18540 at 1931.415 kb on + strand, within PGA1_c18540 at 1931.415 kb on - strand, within PGA1_c18540 at 1931.433 kb on + strand, within PGA1_c18540 at 1931.441 kb on - strand, within PGA1_c18540 at 1931.441 kb on - strand, within PGA1_c18540 at 1931.470 kb on - strand, within PGA1_c18540 at 1931.498 kb on - strand, within PGA1_c18540 at 1931.519 kb on + strand, within PGA1_c18540 at 1931.537 kb on - strand, within PGA1_c18540 at 1931.557 kb on + strand, within PGA1_c18540 at 1931.557 kb on + strand, within PGA1_c18540 at 1931.565 kb on - strand, within PGA1_c18540 at 1931.568 kb on + strand, within PGA1_c18540 at 1931.612 kb on - strand, within PGA1_c18540 at 1931.612 kb on - strand, within PGA1_c18540 at 1931.636 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.670 kb on + strand, within PGA1_c18540 at 1931.723 kb on - strand, within PGA1_c18540 at 1931.770 kb on - strand, within PGA1_c18540 at 1931.800 kb on - strand, within PGA1_c18540 at 1931.843 kb on + strand, within PGA1_c18540 at 1931.851 kb on - strand, within PGA1_c18540 at 1931.949 kb on + strand, within PGA1_c18540 at 1931.957 kb on - strand, within PGA1_c18540 at 1931.982 kb on - strand, within PGA1_c18540
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,929,728 + -0.4 1,929,755 - -0.2 1,929,763 + -2.9 1,929,763 + +0.5 1,929,764 + +1.5 1,929,772 - +1.8 1,929,777 + +0.0 1,929,783 + -0.0 1,929,783 + -0.6 1,929,783 + -0.4 1,929,791 - PGA1_c18500 0.11 +1.0 1,929,791 - PGA1_c18500 0.11 -0.7 1,929,791 - PGA1_c18500 0.11 +0.4 1,929,795 + PGA1_c18500 0.11 -2.1 1,929,795 + PGA1_c18500 0.11 -1.6 1,929,795 + PGA1_c18500 0.11 -0.8 1,929,796 - PGA1_c18500 0.11 +1.5 1,929,802 + PGA1_c18500 0.13 -1.9 1,929,802 + PGA1_c18500 0.13 -1.2 1,929,810 - PGA1_c18500 0.14 -0.3 1,929,825 + PGA1_c18500 0.17 -2.0 1,929,865 + PGA1_c18500 0.24 +0.0 1,929,871 - PGA1_c18500 0.25 -1.6 1,929,871 - PGA1_c18500 0.25 -0.6 1,929,871 - PGA1_c18500 0.25 +0.5 1,929,896 - PGA1_c18500 0.29 -0.4 1,929,898 + PGA1_c18500 0.29 -0.6 1,929,909 + PGA1_c18500 0.31 +0.4 1,929,909 + PGA1_c18500 0.31 +0.7 1,929,940 - PGA1_c18500 0.37 +0.7 1,929,942 - PGA1_c18500 0.37 +0.4 1,930,041 + PGA1_c18500 0.55 -0.9 1,930,041 - PGA1_c18500 0.55 -1.1 1,930,041 - PGA1_c18500 0.55 +0.6 1,930,041 - PGA1_c18500 0.55 -0.4 1,930,041 - PGA1_c18500 0.55 +0.5 1,930,098 + PGA1_c18500 0.65 +0.4 1,930,098 - PGA1_c18500 0.65 +0.5 1,930,106 - PGA1_c18500 0.66 +0.5 1,930,106 - PGA1_c18500 0.66 +0.4 1,930,106 - PGA1_c18500 0.66 -0.7 1,930,106 - PGA1_c18500 0.66 +0.4 1,930,110 + PGA1_c18500 0.67 -0.2 1,930,124 - PGA1_c18500 0.69 -1.5 1,930,161 + PGA1_c18500 0.76 +0.2 1,930,161 + PGA1_c18500 0.76 -0.1 1,930,169 - PGA1_c18500 0.77 -0.3 1,930,173 + PGA1_c18500 0.78 +1.2 1,930,174 - PGA1_c18500 0.78 +0.0 1,930,210 - PGA1_c18500 0.84 +0.8 1,930,211 - PGA1_c18500 0.85 +0.7 1,930,225 - PGA1_c18500 0.87 +0.5 1,930,230 - PGA1_c18500 0.88 -1.1 1,930,267 + -0.0 1,930,268 - -1.2 1,930,272 + -0.6 1,930,275 - +0.3 1,930,275 - +0.5 1,930,280 - -1.6 1,930,334 + +1.2 1,930,334 + -2.0 1,930,342 - -0.1 1,930,362 + PGA1_c18510 0.15 +0.5 1,930,386 + PGA1_c18510 0.20 +0.1 1,930,488 - PGA1_c18510 0.45 -0.2 1,930,508 + PGA1_c18510 0.50 +0.5 1,930,535 + PGA1_c18510 0.56 -0.9 1,930,567 + PGA1_c18510 0.64 +0.3 1,930,567 + PGA1_c18510 0.64 +0.4 1,930,575 - PGA1_c18510 0.66 +0.4 1,930,582 + PGA1_c18510 0.67 -0.1 1,930,652 + PGA1_c18510 0.84 -1.5 1,930,658 + PGA1_c18510 0.86 +1.1 1,930,663 + PGA1_c18510 0.87 +0.6 1,930,674 - PGA1_c18510 0.89 +0.6 1,930,686 + +0.5 1,930,686 + +0.8 1,930,686 + +1.5 1,930,686 + -1.3 1,930,694 - -1.6 1,930,701 + +0.1 1,930,718 - -0.0 1,930,724 + -0.2 1,930,732 - -0.5 1,930,738 - -0.0 1,930,828 + PGA1_c18520 0.39 +1.8 1,930,844 - PGA1_c18520 0.45 +0.2 1,930,860 + PGA1_c18520 0.51 +0.5 1,930,860 + PGA1_c18520 0.51 +0.3 1,930,902 + PGA1_c18520 0.67 +0.5 1,930,910 - PGA1_c18520 0.70 +2.9 1,930,911 + PGA1_c18520 0.70 +1.4 1,930,920 - PGA1_c18520 0.74 -2.0 1,930,941 - PGA1_c18520 0.82 +0.4 1,930,962 - -2.0 1,930,996 + +0.7 1,930,996 - +0.8 1,931,036 - PGA1_c18530 0.14 -0.9 1,931,056 + PGA1_c18530 0.20 -0.4 1,931,064 - PGA1_c18530 0.23 +0.4 1,931,148 + PGA1_c18530 0.50 -0.8 1,931,158 + PGA1_c18530 0.53 +0.4 1,931,188 + PGA1_c18530 0.63 -0.6 1,931,200 - PGA1_c18530 0.67 -1.0 1,931,232 - PGA1_c18530 0.77 +0.6 1,931,311 + +0.5 1,931,319 - -0.5 1,931,319 - +0.9 1,931,378 + +0.8 1,931,383 + -0.2 1,931,409 - PGA1_c18540 0.11 -2.2 1,931,415 + PGA1_c18540 0.12 +0.6 1,931,415 - PGA1_c18540 0.12 -1.3 1,931,433 + PGA1_c18540 0.14 +0.2 1,931,441 - PGA1_c18540 0.15 -0.5 1,931,441 - PGA1_c18540 0.15 +0.2 1,931,470 - PGA1_c18540 0.18 +0.4 1,931,498 - PGA1_c18540 0.21 -1.6 1,931,519 + PGA1_c18540 0.23 -1.4 1,931,537 - PGA1_c18540 0.25 -0.0 1,931,557 + PGA1_c18540 0.27 +0.2 1,931,557 + PGA1_c18540 0.27 -0.6 1,931,565 - PGA1_c18540 0.28 +0.4 1,931,568 + PGA1_c18540 0.28 -0.6 1,931,612 - PGA1_c18540 0.33 +1.1 1,931,612 - PGA1_c18540 0.33 +0.2 1,931,636 + PGA1_c18540 0.35 -0.8 1,931,665 + PGA1_c18540 0.38 +0.8 1,931,665 + PGA1_c18540 0.38 -0.2 1,931,665 + PGA1_c18540 0.38 +0.2 1,931,670 + PGA1_c18540 0.39 -1.8 1,931,723 - PGA1_c18540 0.45 +0.5 1,931,770 - PGA1_c18540 0.49 -0.4 1,931,800 - PGA1_c18540 0.53 -2.3 1,931,843 + PGA1_c18540 0.57 -1.0 1,931,851 - PGA1_c18540 0.58 -0.6 1,931,949 + PGA1_c18540 0.68 +0.8 1,931,957 - PGA1_c18540 0.69 -1.1 1,931,982 - PGA1_c18540 0.72 -0.1
Or see this region's nucleotide sequence