Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16670 and PGA1_c16680 are separated by 42 nucleotides PGA1_c16680 and PGA1_c16690 are separated by 11 nucleotides PGA1_c16690 and PGA1_c16700 are separated by 9 nucleotides
PGA1_c16670: PGA1_c16670 - D-mannose isomerase (EC 5.3.1.7) (from data) , at 1,735,219 to 1,736,472
_c16670
PGA1_c16680: PGA1_c16680 - sugar ABC transporter, ATP-binding protein, at 1,736,515 to 1,737,582
_c16680
PGA1_c16690: PGA1_c16690 - binding protein-dependent transport system, inner membrane component, at 1,737,594 to 1,738,445
_c16690
PGA1_c16700: PGA1_c16700 - binding protein-dependent transport system, inner membrane component, at 1,738,455 to 1,739,405
_c16700
Position (kb)
1736
1737
1738 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1735.524 kb on - strand, within PGA1_c16670 at 1735.634 kb on + strand, within PGA1_c16670 at 1735.694 kb on - strand, within PGA1_c16670 at 1735.703 kb on + strand, within PGA1_c16670 at 1735.708 kb on + strand, within PGA1_c16670 at 1735.708 kb on + strand, within PGA1_c16670 at 1735.726 kb on - strand, within PGA1_c16670 at 1735.728 kb on - strand, within PGA1_c16670 at 1735.739 kb on + strand, within PGA1_c16670 at 1735.770 kb on - strand, within PGA1_c16670 at 1735.777 kb on - strand, within PGA1_c16670 at 1735.787 kb on + strand, within PGA1_c16670 at 1735.793 kb on - strand, within PGA1_c16670 at 1735.795 kb on - strand, within PGA1_c16670 at 1735.839 kb on - strand, within PGA1_c16670 at 1735.893 kb on + strand, within PGA1_c16670 at 1735.901 kb on - strand, within PGA1_c16670 at 1735.988 kb on + strand, within PGA1_c16670 at 1736.096 kb on + strand, within PGA1_c16670 at 1736.104 kb on - strand, within PGA1_c16670 at 1736.180 kb on + strand, within PGA1_c16670 at 1736.200 kb on - strand, within PGA1_c16670 at 1736.200 kb on - strand, within PGA1_c16670 at 1736.268 kb on - strand, within PGA1_c16670 at 1736.281 kb on - strand, within PGA1_c16670 at 1736.327 kb on + strand, within PGA1_c16670 at 1736.329 kb on + strand, within PGA1_c16670 at 1736.335 kb on + strand, within PGA1_c16670 at 1736.409 kb on - strand at 1736.426 kb on - strand at 1736.520 kb on + strand at 1736.527 kb on + strand at 1736.532 kb on + strand at 1736.535 kb on - strand at 1736.584 kb on + strand at 1736.584 kb on + strand at 1736.592 kb on - strand at 1736.595 kb on + strand at 1736.605 kb on + strand at 1736.640 kb on + strand, within PGA1_c16680 at 1736.640 kb on + strand, within PGA1_c16680 at 1736.640 kb on + strand, within PGA1_c16680 at 1736.640 kb on + strand, within PGA1_c16680 at 1736.648 kb on - strand, within PGA1_c16680 at 1736.648 kb on - strand, within PGA1_c16680 at 1736.648 kb on - strand, within PGA1_c16680 at 1736.971 kb on + strand, within PGA1_c16680 at 1736.989 kb on - strand, within PGA1_c16680 at 1736.994 kb on - strand, within PGA1_c16680 at 1737.148 kb on + strand, within PGA1_c16680 at 1737.156 kb on - strand, within PGA1_c16680 at 1737.156 kb on - strand, within PGA1_c16680 at 1737.156 kb on - strand, within PGA1_c16680 at 1737.156 kb on - strand, within PGA1_c16680 at 1737.185 kb on + strand, within PGA1_c16680 at 1737.185 kb on + strand, within PGA1_c16680 at 1737.258 kb on + strand, within PGA1_c16680 at 1737.337 kb on - strand, within PGA1_c16680 at 1737.377 kb on - strand, within PGA1_c16680 at 1737.408 kb on + strand, within PGA1_c16680 at 1737.599 kb on - strand at 1737.615 kb on + strand at 1737.616 kb on + strand at 1737.616 kb on - strand at 1737.626 kb on + strand at 1737.631 kb on + strand at 1737.631 kb on + strand at 1737.631 kb on + strand at 1737.639 kb on - strand at 1737.639 kb on - strand at 1737.639 kb on - strand at 1737.706 kb on - strand, within PGA1_c16690 at 1737.732 kb on + strand, within PGA1_c16690 at 1737.740 kb on - strand, within PGA1_c16690 at 1737.800 kb on - strand, within PGA1_c16690 at 1737.805 kb on - strand, within PGA1_c16690 at 1737.805 kb on - strand, within PGA1_c16690 at 1737.821 kb on - strand, within PGA1_c16690 at 1737.838 kb on - strand, within PGA1_c16690 at 1737.855 kb on - strand, within PGA1_c16690 at 1737.987 kb on + strand, within PGA1_c16690 at 1737.988 kb on + strand, within PGA1_c16690 at 1737.995 kb on - strand, within PGA1_c16690 at 1737.996 kb on - strand, within PGA1_c16690 at 1738.025 kb on + strand, within PGA1_c16690 at 1738.032 kb on - strand, within PGA1_c16690 at 1738.033 kb on - strand, within PGA1_c16690 at 1738.080 kb on + strand, within PGA1_c16690 at 1738.088 kb on - strand, within PGA1_c16690 at 1738.144 kb on + strand, within PGA1_c16690 at 1738.144 kb on + strand, within PGA1_c16690 at 1738.242 kb on - strand, within PGA1_c16690 at 1738.295 kb on + strand, within PGA1_c16690 at 1738.305 kb on + strand, within PGA1_c16690 at 1738.321 kb on - strand, within PGA1_c16690 at 1738.348 kb on + strand, within PGA1_c16690 at 1738.356 kb on - strand, within PGA1_c16690 at 1738.356 kb on - strand, within PGA1_c16690 at 1738.356 kb on - strand, within PGA1_c16690 at 1738.365 kb on + strand at 1738.366 kb on - strand at 1738.420 kb on - strand at 1738.443 kb on - strand at 1738.466 kb on - strand at 1738.490 kb on + strand at 1738.490 kb on + strand at 1738.498 kb on + strand at 1738.506 kb on - strand at 1738.521 kb on - strand at 1738.536 kb on + strand at 1738.536 kb on + strand at 1738.536 kb on + strand at 1738.536 kb on + strand at 1738.544 kb on - strand at 1738.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,735,524 - PGA1_c16670 0.24 +0.5 1,735,634 + PGA1_c16670 0.33 +0.6 1,735,694 - PGA1_c16670 0.38 -0.0 1,735,703 + PGA1_c16670 0.39 -1.2 1,735,708 + PGA1_c16670 0.39 +1.3 1,735,708 + PGA1_c16670 0.39 -0.4 1,735,726 - PGA1_c16670 0.40 -1.7 1,735,728 - PGA1_c16670 0.41 -0.4 1,735,739 + PGA1_c16670 0.41 +0.5 1,735,770 - PGA1_c16670 0.44 -0.1 1,735,777 - PGA1_c16670 0.44 -0.2 1,735,787 + PGA1_c16670 0.45 -0.2 1,735,793 - PGA1_c16670 0.46 +0.7 1,735,795 - PGA1_c16670 0.46 +0.5 1,735,839 - PGA1_c16670 0.49 -0.3 1,735,893 + PGA1_c16670 0.54 +1.0 1,735,901 - PGA1_c16670 0.54 -0.9 1,735,988 + PGA1_c16670 0.61 -0.5 1,736,096 + PGA1_c16670 0.70 +0.6 1,736,104 - PGA1_c16670 0.71 -0.6 1,736,180 + PGA1_c16670 0.77 +0.1 1,736,200 - PGA1_c16670 0.78 +0.3 1,736,200 - PGA1_c16670 0.78 -0.2 1,736,268 - PGA1_c16670 0.84 -0.8 1,736,281 - PGA1_c16670 0.85 -0.1 1,736,327 + PGA1_c16670 0.88 -0.3 1,736,329 + PGA1_c16670 0.89 -0.8 1,736,335 + PGA1_c16670 0.89 -0.7 1,736,409 - +0.8 1,736,426 - +0.3 1,736,520 + -0.6 1,736,527 + -0.9 1,736,532 + -0.1 1,736,535 - +0.3 1,736,584 + -0.7 1,736,584 + +0.5 1,736,592 - +0.3 1,736,595 + -0.4 1,736,605 + -0.5 1,736,640 + PGA1_c16680 0.12 +0.7 1,736,640 + PGA1_c16680 0.12 +1.1 1,736,640 + PGA1_c16680 0.12 -0.2 1,736,640 + PGA1_c16680 0.12 +1.1 1,736,648 - PGA1_c16680 0.12 +0.2 1,736,648 - PGA1_c16680 0.12 +1.1 1,736,648 - PGA1_c16680 0.12 +1.3 1,736,971 + PGA1_c16680 0.43 +2.2 1,736,989 - PGA1_c16680 0.44 -1.4 1,736,994 - PGA1_c16680 0.45 +0.4 1,737,148 + PGA1_c16680 0.59 -0.6 1,737,156 - PGA1_c16680 0.60 -0.8 1,737,156 - PGA1_c16680 0.60 +1.1 1,737,156 - PGA1_c16680 0.60 +0.9 1,737,156 - PGA1_c16680 0.60 +0.3 1,737,185 + PGA1_c16680 0.63 +0.7 1,737,185 + PGA1_c16680 0.63 -0.2 1,737,258 + PGA1_c16680 0.70 -1.3 1,737,337 - PGA1_c16680 0.77 +0.9 1,737,377 - PGA1_c16680 0.81 -0.8 1,737,408 + PGA1_c16680 0.84 -0.1 1,737,599 - -0.2 1,737,615 + +0.1 1,737,616 + +0.3 1,737,616 - -0.3 1,737,626 + +1.0 1,737,631 + -0.2 1,737,631 + -0.1 1,737,631 + +0.4 1,737,639 - -0.3 1,737,639 - -0.4 1,737,639 - -2.3 1,737,706 - PGA1_c16690 0.13 +0.1 1,737,732 + PGA1_c16690 0.16 +0.4 1,737,740 - PGA1_c16690 0.17 +0.1 1,737,800 - PGA1_c16690 0.24 +0.2 1,737,805 - PGA1_c16690 0.25 +0.8 1,737,805 - PGA1_c16690 0.25 +0.4 1,737,821 - PGA1_c16690 0.27 +0.3 1,737,838 - PGA1_c16690 0.29 +0.1 1,737,855 - PGA1_c16690 0.31 -0.2 1,737,987 + PGA1_c16690 0.46 -1.1 1,737,988 + PGA1_c16690 0.46 +1.0 1,737,995 - PGA1_c16690 0.47 -0.6 1,737,996 - PGA1_c16690 0.47 -0.5 1,738,025 + PGA1_c16690 0.51 -0.3 1,738,032 - PGA1_c16690 0.51 -1.4 1,738,033 - PGA1_c16690 0.52 +0.3 1,738,080 + PGA1_c16690 0.57 -0.7 1,738,088 - PGA1_c16690 0.58 +0.4 1,738,144 + PGA1_c16690 0.65 -0.2 1,738,144 + PGA1_c16690 0.65 +0.5 1,738,242 - PGA1_c16690 0.76 -0.1 1,738,295 + PGA1_c16690 0.82 -0.4 1,738,305 + PGA1_c16690 0.83 +0.6 1,738,321 - PGA1_c16690 0.85 +0.6 1,738,348 + PGA1_c16690 0.88 +1.4 1,738,356 - PGA1_c16690 0.89 -0.8 1,738,356 - PGA1_c16690 0.89 +0.1 1,738,356 - PGA1_c16690 0.89 +0.1 1,738,365 + -0.5 1,738,366 - -0.5 1,738,420 - +1.5 1,738,443 - -0.1 1,738,466 - -0.4 1,738,490 + +1.3 1,738,490 + -0.2 1,738,498 + -0.4 1,738,506 - -1.3 1,738,521 - -0.0 1,738,536 + -1.7 1,738,536 + +0.6 1,738,536 + +0.6 1,738,536 + -0.3 1,738,544 - -1.1 1,738,544 - -0.6
Or see this region's nucleotide sequence