Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13610 and PGA1_c13620 are separated by 300 nucleotides PGA1_c13620 and PGA1_c13630 are separated by 203 nucleotides PGA1_c13630 and PGA1_c13640 are separated by 24 nucleotides PGA1_c13640 and PGA1_c13650 overlap by 8 nucleotides PGA1_c13650 and PGA1_c13660 are separated by 2 nucleotides
PGA1_c13610: PGA1_c13610 - ABC-type transport system, involved in lipoprotein release, permease component, at 1,406,301 to 1,407,503
_c13610
PGA1_c13620: PGA1_c13620 - hypothetical protein, at 1,407,804 to 1,407,971
_c13620
PGA1_c13630: PGA1_c13630 - 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal., at 1,408,175 to 1,409,122
_c13630
PGA1_c13640: PGA1_c13640 - Phosphopantetheine attachment site., at 1,409,147 to 1,409,407
_c13640
PGA1_c13650: PGA1_c13650 - hypothetical protein, at 1,409,400 to 1,410,080
_c13650
PGA1_c13660: PGA1_c13660 - hypothetical protein, at 1,410,083 to 1,410,619
_c13660
Position (kb)
1408
1409
1410 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1407.453 kb on - strand at 1407.504 kb on + strand at 1407.514 kb on + strand at 1407.514 kb on + strand at 1407.514 kb on + strand at 1407.642 kb on + strand at 1407.650 kb on - strand at 1407.672 kb on - strand at 1407.676 kb on - strand at 1407.683 kb on - strand at 1407.695 kb on + strand at 1407.735 kb on + strand at 1407.735 kb on + strand at 1407.735 kb on + strand at 1407.737 kb on - strand at 1407.743 kb on - strand at 1407.743 kb on - strand at 1407.743 kb on - strand at 1407.743 kb on - strand at 1407.743 kb on - strand at 1407.743 kb on - strand at 1407.747 kb on - strand at 1407.754 kb on + strand at 1407.754 kb on + strand at 1407.754 kb on + strand at 1407.754 kb on + strand at 1407.754 kb on + strand at 1407.754 kb on + strand at 1407.762 kb on - strand at 1407.762 kb on - strand at 1407.762 kb on - strand at 1407.762 kb on - strand at 1407.779 kb on + strand at 1407.782 kb on + strand at 1407.803 kb on + strand at 1407.807 kb on - strand at 1407.843 kb on + strand, within PGA1_c13620 at 1407.848 kb on + strand, within PGA1_c13620 at 1407.905 kb on + strand, within PGA1_c13620 at 1407.926 kb on + strand, within PGA1_c13620 at 1407.929 kb on - strand, within PGA1_c13620 at 1407.934 kb on - strand, within PGA1_c13620 at 1407.934 kb on - strand, within PGA1_c13620 at 1407.983 kb on - strand at 1407.992 kb on + strand at 1407.992 kb on + strand at 1407.992 kb on + strand at 1408.000 kb on - strand at 1408.038 kb on + strand at 1408.059 kb on + strand at 1408.059 kb on + strand at 1408.059 kb on + strand at 1408.067 kb on - strand at 1408.110 kb on + strand at 1408.149 kb on + strand at 1408.149 kb on + strand at 1408.157 kb on - strand at 1408.157 kb on - strand at 1408.177 kb on + strand at 1408.182 kb on + strand at 1408.244 kb on - strand at 1408.248 kb on + strand at 1408.248 kb on + strand at 1408.248 kb on + strand at 1408.248 kb on + strand at 1408.248 kb on + strand at 1408.253 kb on + strand at 1408.255 kb on + strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.256 kb on - strand at 1408.260 kb on + strand at 1408.277 kb on - strand, within PGA1_c13630 at 1408.283 kb on - strand, within PGA1_c13630 at 1408.325 kb on + strand, within PGA1_c13630 at 1408.325 kb on + strand, within PGA1_c13630 at 1408.333 kb on - strand, within PGA1_c13630 at 1408.357 kb on - strand, within PGA1_c13630 at 1408.401 kb on - strand, within PGA1_c13630 at 1408.403 kb on - strand, within PGA1_c13630 at 1408.427 kb on + strand, within PGA1_c13630 at 1408.427 kb on + strand, within PGA1_c13630 at 1408.435 kb on - strand, within PGA1_c13630 at 1408.435 kb on - strand, within PGA1_c13630 at 1408.435 kb on - strand, within PGA1_c13630 at 1408.435 kb on - strand, within PGA1_c13630 at 1408.442 kb on + strand, within PGA1_c13630 at 1408.442 kb on + strand, within PGA1_c13630 at 1408.442 kb on + strand, within PGA1_c13630 at 1408.442 kb on + strand, within PGA1_c13630 at 1408.447 kb on + strand, within PGA1_c13630 at 1408.450 kb on - strand, within PGA1_c13630 at 1408.450 kb on - strand, within PGA1_c13630 at 1408.450 kb on - strand, within PGA1_c13630 at 1408.450 kb on - strand, within PGA1_c13630 at 1408.477 kb on - strand, within PGA1_c13630 at 1408.524 kb on + strand, within PGA1_c13630 at 1408.553 kb on - strand, within PGA1_c13630 at 1408.575 kb on - strand, within PGA1_c13630 at 1408.621 kb on - strand, within PGA1_c13630 at 1408.651 kb on + strand, within PGA1_c13630 at 1408.659 kb on - strand, within PGA1_c13630 at 1408.674 kb on - strand, within PGA1_c13630 at 1408.701 kb on + strand, within PGA1_c13630 at 1408.706 kb on + strand, within PGA1_c13630 at 1408.711 kb on + strand, within PGA1_c13630 at 1408.713 kb on + strand, within PGA1_c13630 at 1408.714 kb on - strand, within PGA1_c13630 at 1408.716 kb on - strand, within PGA1_c13630 at 1408.719 kb on - strand, within PGA1_c13630 at 1408.721 kb on - strand, within PGA1_c13630 at 1408.749 kb on + strand, within PGA1_c13630 at 1408.758 kb on - strand, within PGA1_c13630 at 1408.775 kb on - strand, within PGA1_c13630 at 1408.792 kb on - strand, within PGA1_c13630 at 1408.815 kb on + strand, within PGA1_c13630 at 1408.815 kb on + strand, within PGA1_c13630 at 1408.820 kb on + strand, within PGA1_c13630 at 1408.820 kb on + strand, within PGA1_c13630 at 1408.820 kb on + strand, within PGA1_c13630 at 1408.828 kb on - strand, within PGA1_c13630 at 1408.892 kb on - strand, within PGA1_c13630 at 1408.892 kb on - strand, within PGA1_c13630 at 1409.043 kb on + strand at 1409.043 kb on + strand at 1409.045 kb on + strand at 1409.045 kb on + strand at 1409.051 kb on - strand at 1409.053 kb on - strand at 1409.058 kb on - strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.075 kb on + strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.083 kb on - strand at 1409.092 kb on - strand at 1409.106 kb on - strand at 1409.166 kb on + strand at 1409.176 kb on + strand, within PGA1_c13640 at 1409.176 kb on + strand, within PGA1_c13640 at 1409.184 kb on - strand, within PGA1_c13640 at 1409.205 kb on - strand, within PGA1_c13640 at 1409.230 kb on - strand, within PGA1_c13640 at 1409.235 kb on - strand, within PGA1_c13640 at 1409.285 kb on - strand, within PGA1_c13640 at 1409.305 kb on + strand, within PGA1_c13640 at 1409.319 kb on + strand, within PGA1_c13640 at 1409.319 kb on + strand, within PGA1_c13640 at 1409.327 kb on - strand, within PGA1_c13640 at 1409.327 kb on - strand, within PGA1_c13640 at 1409.341 kb on - strand, within PGA1_c13640 at 1409.354 kb on - strand, within PGA1_c13640 at 1409.374 kb on + strand, within PGA1_c13640 at 1409.392 kb on + strand at 1409.392 kb on + strand at 1409.400 kb on - strand at 1409.401 kb on + strand at 1409.401 kb on + strand at 1409.406 kb on + strand at 1409.409 kb on - strand at 1409.416 kb on + strand at 1409.416 kb on + strand at 1409.426 kb on - strand at 1409.455 kb on + strand at 1409.497 kb on + strand, within PGA1_c13650 at 1409.530 kb on + strand, within PGA1_c13650 at 1409.532 kb on - strand, within PGA1_c13650 at 1409.543 kb on - strand, within PGA1_c13650 at 1409.568 kb on - strand, within PGA1_c13650 at 1409.578 kb on + strand, within PGA1_c13650 at 1409.578 kb on + strand, within PGA1_c13650 at 1409.586 kb on - strand, within PGA1_c13650 at 1409.586 kb on - strand, within PGA1_c13650 at 1409.638 kb on + strand, within PGA1_c13650 at 1409.655 kb on - strand, within PGA1_c13650 at 1409.689 kb on + strand, within PGA1_c13650 at 1409.740 kb on - strand, within PGA1_c13650 at 1409.749 kb on + strand, within PGA1_c13650 at 1409.749 kb on + strand, within PGA1_c13650 at 1409.749 kb on + strand, within PGA1_c13650 at 1409.749 kb on + strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.757 kb on - strand, within PGA1_c13650 at 1409.770 kb on + strand, within PGA1_c13650 at 1409.770 kb on + strand, within PGA1_c13650 at 1409.770 kb on + strand, within PGA1_c13650 at 1409.780 kb on - strand, within PGA1_c13650 at 1409.788 kb on - strand, within PGA1_c13650 at 1409.845 kb on + strand, within PGA1_c13650 at 1409.869 kb on - strand, within PGA1_c13650 at 1409.872 kb on - strand, within PGA1_c13650 at 1409.894 kb on - strand, within PGA1_c13650 at 1409.902 kb on + strand, within PGA1_c13650 at 1409.910 kb on - strand, within PGA1_c13650 at 1409.910 kb on - strand, within PGA1_c13650 at 1409.912 kb on - strand, within PGA1_c13650 at 1409.919 kb on - strand, within PGA1_c13650 at 1409.968 kb on + strand, within PGA1_c13650 at 1409.976 kb on - strand, within PGA1_c13650 at 1409.986 kb on + strand, within PGA1_c13650 at 1410.007 kb on + strand, within PGA1_c13650 at 1410.032 kb on + strand at 1410.032 kb on + strand at 1410.032 kb on + strand at 1410.042 kb on + strand at 1410.050 kb on - strand at 1410.074 kb on + strand at 1410.074 kb on + strand at 1410.079 kb on + strand at 1410.079 kb on + strand at 1410.079 kb on + strand at 1410.082 kb on - strand at 1410.082 kb on - strand at 1410.087 kb on - strand at 1410.087 kb on - strand at 1410.087 kb on - strand at 1410.087 kb on - strand at 1410.088 kb on + strand at 1410.089 kb on + strand at 1410.096 kb on - strand at 1410.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,407,453 - -0.5 1,407,504 + -0.2 1,407,514 + +0.3 1,407,514 + +1.8 1,407,514 + -0.7 1,407,642 + -0.7 1,407,650 - +0.2 1,407,672 - +0.5 1,407,676 - +0.0 1,407,683 - +1.8 1,407,695 + +0.8 1,407,735 + -0.2 1,407,735 + +1.7 1,407,735 + -0.3 1,407,737 - -0.8 1,407,743 - -0.9 1,407,743 - -0.5 1,407,743 - +0.2 1,407,743 - +1.8 1,407,743 - +0.4 1,407,743 - -0.4 1,407,747 - -0.4 1,407,754 + +0.5 1,407,754 + +0.6 1,407,754 + -1.0 1,407,754 + +1.0 1,407,754 + +1.2 1,407,754 + -0.0 1,407,762 - -0.4 1,407,762 - +1.6 1,407,762 - -2.2 1,407,762 - +0.4 1,407,779 + +0.1 1,407,782 + -0.3 1,407,803 + +1.7 1,407,807 - -0.2 1,407,843 + PGA1_c13620 0.23 +0.5 1,407,848 + PGA1_c13620 0.26 -1.3 1,407,905 + PGA1_c13620 0.60 +0.5 1,407,926 + PGA1_c13620 0.73 +0.4 1,407,929 - PGA1_c13620 0.74 -0.2 1,407,934 - PGA1_c13620 0.77 +0.4 1,407,934 - PGA1_c13620 0.77 -1.1 1,407,983 - -1.0 1,407,992 + +2.3 1,407,992 + -1.7 1,407,992 + -0.1 1,408,000 - +0.7 1,408,038 + -1.8 1,408,059 + -0.6 1,408,059 + +0.3 1,408,059 + -0.1 1,408,067 - +1.0 1,408,110 + -0.8 1,408,149 + -0.6 1,408,149 + +0.0 1,408,157 - -1.4 1,408,157 - +0.4 1,408,177 + +0.4 1,408,182 + -0.6 1,408,244 - +0.5 1,408,248 + -1.2 1,408,248 + -0.2 1,408,248 + -1.6 1,408,248 + +0.2 1,408,248 + -0.4 1,408,253 + -0.8 1,408,255 + +1.0 1,408,256 - +0.7 1,408,256 - +2.0 1,408,256 - +0.8 1,408,256 - +0.4 1,408,256 - -2.6 1,408,256 - -1.3 1,408,256 - +0.7 1,408,256 - +1.0 1,408,256 - +1.4 1,408,260 + -0.6 1,408,277 - PGA1_c13630 0.11 +0.4 1,408,283 - PGA1_c13630 0.11 +0.6 1,408,325 + PGA1_c13630 0.16 +0.3 1,408,325 + PGA1_c13630 0.16 +0.7 1,408,333 - PGA1_c13630 0.17 +0.2 1,408,357 - PGA1_c13630 0.19 +0.7 1,408,401 - PGA1_c13630 0.24 -0.1 1,408,403 - PGA1_c13630 0.24 +0.0 1,408,427 + PGA1_c13630 0.27 +0.7 1,408,427 + PGA1_c13630 0.27 +0.1 1,408,435 - PGA1_c13630 0.27 -0.3 1,408,435 - PGA1_c13630 0.27 -0.6 1,408,435 - PGA1_c13630 0.27 +0.4 1,408,435 - PGA1_c13630 0.27 +1.3 1,408,442 + PGA1_c13630 0.28 +1.1 1,408,442 + PGA1_c13630 0.28 +0.1 1,408,442 + PGA1_c13630 0.28 +1.6 1,408,442 + PGA1_c13630 0.28 -0.3 1,408,447 + PGA1_c13630 0.29 +0.4 1,408,450 - PGA1_c13630 0.29 -1.5 1,408,450 - PGA1_c13630 0.29 +0.3 1,408,450 - PGA1_c13630 0.29 +0.8 1,408,450 - PGA1_c13630 0.29 -0.4 1,408,477 - PGA1_c13630 0.32 +0.1 1,408,524 + PGA1_c13630 0.37 +0.5 1,408,553 - PGA1_c13630 0.40 -0.4 1,408,575 - PGA1_c13630 0.42 +0.1 1,408,621 - PGA1_c13630 0.47 -0.6 1,408,651 + PGA1_c13630 0.50 -0.6 1,408,659 - PGA1_c13630 0.51 +0.6 1,408,674 - PGA1_c13630 0.53 +1.1 1,408,701 + PGA1_c13630 0.55 +0.7 1,408,706 + PGA1_c13630 0.56 -1.8 1,408,711 + PGA1_c13630 0.57 +0.7 1,408,713 + PGA1_c13630 0.57 +0.8 1,408,714 - PGA1_c13630 0.57 -0.8 1,408,716 - PGA1_c13630 0.57 +2.0 1,408,719 - PGA1_c13630 0.57 +1.2 1,408,721 - PGA1_c13630 0.58 -0.0 1,408,749 + PGA1_c13630 0.61 +0.7 1,408,758 - PGA1_c13630 0.61 +1.6 1,408,775 - PGA1_c13630 0.63 -0.3 1,408,792 - PGA1_c13630 0.65 -1.4 1,408,815 + PGA1_c13630 0.68 +0.9 1,408,815 + PGA1_c13630 0.68 -2.0 1,408,820 + PGA1_c13630 0.68 -2.3 1,408,820 + PGA1_c13630 0.68 +0.9 1,408,820 + PGA1_c13630 0.68 +0.1 1,408,828 - PGA1_c13630 0.69 +0.4 1,408,892 - PGA1_c13630 0.76 -1.1 1,408,892 - PGA1_c13630 0.76 -0.4 1,409,043 + -1.2 1,409,043 + -1.6 1,409,045 + -0.8 1,409,045 + -1.2 1,409,051 - -1.1 1,409,053 - +0.6 1,409,058 - -0.1 1,409,075 + -0.4 1,409,075 + -0.2 1,409,075 + -0.3 1,409,075 + -1.0 1,409,075 + +1.0 1,409,075 + +0.0 1,409,075 + -0.0 1,409,075 + +0.9 1,409,075 + -0.8 1,409,075 + +0.0 1,409,075 + +0.6 1,409,083 - -0.8 1,409,083 - -0.5 1,409,083 - -1.0 1,409,083 - +0.4 1,409,083 - +0.1 1,409,083 - +1.0 1,409,083 - -1.1 1,409,083 - -0.4 1,409,083 - +0.2 1,409,083 - -0.6 1,409,083 - -1.2 1,409,083 - +1.3 1,409,083 - +0.8 1,409,083 - -0.2 1,409,083 - -0.9 1,409,083 - +0.3 1,409,083 - +0.6 1,409,083 - +0.4 1,409,092 - -0.2 1,409,106 - +0.5 1,409,166 + +0.5 1,409,176 + PGA1_c13640 0.11 +0.2 1,409,176 + PGA1_c13640 0.11 -0.5 1,409,184 - PGA1_c13640 0.14 +1.0 1,409,205 - PGA1_c13640 0.22 -0.1 1,409,230 - PGA1_c13640 0.32 -0.2 1,409,235 - PGA1_c13640 0.34 -0.0 1,409,285 - PGA1_c13640 0.53 -0.1 1,409,305 + PGA1_c13640 0.61 +1.7 1,409,319 + PGA1_c13640 0.66 +0.2 1,409,319 + PGA1_c13640 0.66 +1.4 1,409,327 - PGA1_c13640 0.69 -0.8 1,409,327 - PGA1_c13640 0.69 -0.2 1,409,341 - PGA1_c13640 0.74 +0.0 1,409,354 - PGA1_c13640 0.79 +0.2 1,409,374 + PGA1_c13640 0.87 +1.0 1,409,392 + -0.2 1,409,392 + +0.0 1,409,400 - -1.8 1,409,401 + +0.3 1,409,401 + +0.6 1,409,406 + +0.8 1,409,409 - +0.9 1,409,416 + +0.6 1,409,416 + +1.4 1,409,426 - -0.4 1,409,455 + +0.5 1,409,497 + PGA1_c13650 0.14 +0.2 1,409,530 + PGA1_c13650 0.19 -0.3 1,409,532 - PGA1_c13650 0.19 +0.1 1,409,543 - PGA1_c13650 0.21 -0.6 1,409,568 - PGA1_c13650 0.25 -0.1 1,409,578 + PGA1_c13650 0.26 -0.2 1,409,578 + PGA1_c13650 0.26 -0.5 1,409,586 - PGA1_c13650 0.27 -0.8 1,409,586 - PGA1_c13650 0.27 +1.1 1,409,638 + PGA1_c13650 0.35 +1.2 1,409,655 - PGA1_c13650 0.37 +0.3 1,409,689 + PGA1_c13650 0.42 -0.1 1,409,740 - PGA1_c13650 0.50 +0.4 1,409,749 + PGA1_c13650 0.51 -0.2 1,409,749 + PGA1_c13650 0.51 +0.9 1,409,749 + PGA1_c13650 0.51 +1.0 1,409,749 + PGA1_c13650 0.51 -1.2 1,409,757 - PGA1_c13650 0.52 -0.1 1,409,757 - PGA1_c13650 0.52 +0.8 1,409,757 - PGA1_c13650 0.52 -0.9 1,409,757 - PGA1_c13650 0.52 -2.2 1,409,757 - PGA1_c13650 0.52 +1.0 1,409,757 - PGA1_c13650 0.52 -0.2 1,409,757 - PGA1_c13650 0.52 +0.4 1,409,757 - PGA1_c13650 0.52 -0.6 1,409,770 + PGA1_c13650 0.54 +0.9 1,409,770 + PGA1_c13650 0.54 +0.9 1,409,770 + PGA1_c13650 0.54 +1.0 1,409,780 - PGA1_c13650 0.56 -1.4 1,409,788 - PGA1_c13650 0.57 -0.4 1,409,845 + PGA1_c13650 0.65 -0.1 1,409,869 - PGA1_c13650 0.69 +0.8 1,409,872 - PGA1_c13650 0.69 +0.5 1,409,894 - PGA1_c13650 0.73 +0.1 1,409,902 + PGA1_c13650 0.74 +1.1 1,409,910 - PGA1_c13650 0.75 -0.1 1,409,910 - PGA1_c13650 0.75 -0.4 1,409,912 - PGA1_c13650 0.75 -0.2 1,409,919 - PGA1_c13650 0.76 -0.7 1,409,968 + PGA1_c13650 0.83 -0.0 1,409,976 - PGA1_c13650 0.85 -0.4 1,409,986 + PGA1_c13650 0.86 -0.0 1,410,007 + PGA1_c13650 0.89 -0.7 1,410,032 + +0.4 1,410,032 + -0.2 1,410,032 + -1.6 1,410,042 + -0.6 1,410,050 - +0.8 1,410,074 + +0.8 1,410,074 + -0.5 1,410,079 + -0.4 1,410,079 + +0.6 1,410,079 + +0.1 1,410,082 - -1.6 1,410,082 - +0.8 1,410,087 - -0.9 1,410,087 - +0.1 1,410,087 - +0.6 1,410,087 - -0.4 1,410,088 + +0.9 1,410,089 + -0.1 1,410,096 - -0.6 1,410,096 - +1.5
Or see this region's nucleotide sequence