Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00640 and PGA1_c00650 are separated by 109 nucleotides PGA1_c00650 and PGA1_c00660 are separated by 106 nucleotides PGA1_c00660 and PGA1_c00670 overlap by 4 nucleotides
PGA1_c00640: PGA1_c00640 - putative elongation factor G, at 64,008 to 66,002
_c00640
PGA1_c00650: PGA1_c00650 - Uncharacterized conserved protein, at 66,112 to 66,639
_c00650
PGA1_c00660: PGA1_c00660 - transcriptional regulator, lysR family, at 66,746 to 67,624
_c00660
PGA1_c00670: PGA1_c00670 - putative integral membrane protein, at 67,621 to 68,529
_c00670
Position (kb)
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 65.125 kb on + strand, within PGA1_c00640 at 65.127 kb on + strand, within PGA1_c00640 at 65.159 kb on + strand, within PGA1_c00640 at 65.159 kb on + strand, within PGA1_c00640 at 65.167 kb on - strand, within PGA1_c00640 at 65.174 kb on - strand, within PGA1_c00640 at 65.176 kb on + strand, within PGA1_c00640 at 65.250 kb on + strand, within PGA1_c00640 at 65.258 kb on - strand, within PGA1_c00640 at 65.335 kb on + strand, within PGA1_c00640 at 65.335 kb on + strand, within PGA1_c00640 at 65.367 kb on + strand, within PGA1_c00640 at 65.454 kb on + strand, within PGA1_c00640 at 65.462 kb on - strand, within PGA1_c00640 at 65.462 kb on - strand, within PGA1_c00640 at 65.462 kb on - strand, within PGA1_c00640 at 65.507 kb on + strand, within PGA1_c00640 at 65.507 kb on + strand, within PGA1_c00640 at 65.508 kb on + strand, within PGA1_c00640 at 65.515 kb on + strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.515 kb on - strand, within PGA1_c00640 at 65.516 kb on - strand, within PGA1_c00640 at 65.520 kb on + strand, within PGA1_c00640 at 65.523 kb on + strand, within PGA1_c00640 at 65.533 kb on - strand, within PGA1_c00640 at 65.533 kb on - strand, within PGA1_c00640 at 65.533 kb on - strand, within PGA1_c00640 at 65.580 kb on + strand, within PGA1_c00640 at 65.615 kb on - strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.617 kb on + strand, within PGA1_c00640 at 65.633 kb on + strand, within PGA1_c00640 at 65.641 kb on - strand, within PGA1_c00640 at 65.680 kb on + strand, within PGA1_c00640 at 65.716 kb on - strand, within PGA1_c00640 at 65.763 kb on + strand, within PGA1_c00640 at 65.778 kb on - strand, within PGA1_c00640 at 65.799 kb on + strand, within PGA1_c00640 at 65.826 kb on + strand at 65.831 kb on + strand at 65.831 kb on + strand at 65.839 kb on - strand at 65.839 kb on - strand at 65.839 kb on - strand at 65.845 kb on + strand at 65.845 kb on + strand at 65.845 kb on + strand at 65.901 kb on + strand at 65.910 kb on + strand at 65.918 kb on + strand at 65.919 kb on - strand at 66.007 kb on + strand at 66.052 kb on + strand at 66.103 kb on - strand at 66.113 kb on - strand at 66.127 kb on - strand at 66.127 kb on - strand at 66.146 kb on - strand at 66.201 kb on + strand, within PGA1_c00650 at 66.202 kb on - strand, within PGA1_c00650 at 66.209 kb on + strand, within PGA1_c00650 at 66.220 kb on - strand, within PGA1_c00650 at 66.227 kb on + strand, within PGA1_c00650 at 66.235 kb on - strand, within PGA1_c00650 at 66.296 kb on + strand, within PGA1_c00650 at 66.296 kb on + strand, within PGA1_c00650 at 66.336 kb on - strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.338 kb on + strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.346 kb on - strand, within PGA1_c00650 at 66.391 kb on - strand, within PGA1_c00650 at 66.427 kb on + strand, within PGA1_c00650 at 66.442 kb on - strand, within PGA1_c00650 at 66.474 kb on + strand, within PGA1_c00650 at 66.518 kb on - strand, within PGA1_c00650 at 66.518 kb on - strand, within PGA1_c00650 at 66.523 kb on - strand, within PGA1_c00650 at 66.540 kb on - strand, within PGA1_c00650 at 66.569 kb on + strand, within PGA1_c00650 at 66.569 kb on + strand, within PGA1_c00650 at 66.574 kb on + strand, within PGA1_c00650 at 66.577 kb on - strand, within PGA1_c00650 at 66.582 kb on - strand, within PGA1_c00650 at 66.641 kb on - strand at 66.641 kb on - strand at 66.641 kb on - strand at 66.641 kb on - strand at 66.705 kb on + strand at 66.730 kb on - strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.745 kb on - strand at 66.771 kb on - strand at 66.850 kb on - strand, within PGA1_c00660 at 66.871 kb on - strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.991 kb on + strand, within PGA1_c00660 at 66.998 kb on + strand, within PGA1_c00660 at 66.999 kb on + strand, within PGA1_c00660 at 67.102 kb on - strand, within PGA1_c00660 at 67.208 kb on + strand, within PGA1_c00660 at 67.238 kb on + strand, within PGA1_c00660 at 67.267 kb on + strand, within PGA1_c00660 at 67.284 kb on - strand, within PGA1_c00660 at 67.291 kb on - strand, within PGA1_c00660 at 67.329 kb on - strand, within PGA1_c00660 at 67.421 kb on - strand, within PGA1_c00660 at 67.429 kb on + strand, within PGA1_c00660 at 67.429 kb on + strand, within PGA1_c00660 at 67.437 kb on - strand, within PGA1_c00660 at 67.459 kb on - strand, within PGA1_c00660 at 67.487 kb on - strand, within PGA1_c00660 at 67.498 kb on - strand, within PGA1_c00660 at 67.531 kb on - strand, within PGA1_c00660 at 67.550 kb on + strand at 67.550 kb on + strand at 67.558 kb on - strand at 67.571 kb on - strand at 67.595 kb on - strand at 67.609 kb on + strand at 67.609 kb on + strand at 67.617 kb on - strand at 67.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 10 mM remove 65,125 + PGA1_c00640 0.56 +0.7 65,127 + PGA1_c00640 0.56 -0.3 65,159 + PGA1_c00640 0.58 -2.1 65,159 + PGA1_c00640 0.58 -1.0 65,167 - PGA1_c00640 0.58 +0.1 65,174 - PGA1_c00640 0.58 +3.3 65,176 + PGA1_c00640 0.59 -0.4 65,250 + PGA1_c00640 0.62 +0.2 65,258 - PGA1_c00640 0.63 -1.4 65,335 + PGA1_c00640 0.67 -0.4 65,335 + PGA1_c00640 0.67 -0.6 65,367 + PGA1_c00640 0.68 -0.4 65,454 + PGA1_c00640 0.72 -0.1 65,462 - PGA1_c00640 0.73 -0.3 65,462 - PGA1_c00640 0.73 -0.6 65,462 - PGA1_c00640 0.73 +0.1 65,507 + PGA1_c00640 0.75 -0.4 65,507 + PGA1_c00640 0.75 -0.1 65,508 + PGA1_c00640 0.75 +0.6 65,515 + PGA1_c00640 0.76 +3.3 65,515 - PGA1_c00640 0.76 -2.6 65,515 - PGA1_c00640 0.76 -0.5 65,515 - PGA1_c00640 0.76 -2.2 65,515 - PGA1_c00640 0.76 -0.0 65,515 - PGA1_c00640 0.76 -0.1 65,515 - PGA1_c00640 0.76 +0.6 65,516 - PGA1_c00640 0.76 +1.0 65,520 + PGA1_c00640 0.76 +0.9 65,523 + PGA1_c00640 0.76 -0.1 65,533 - PGA1_c00640 0.76 +0.6 65,533 - PGA1_c00640 0.76 +1.1 65,533 - PGA1_c00640 0.76 -0.7 65,580 + PGA1_c00640 0.79 -1.6 65,615 - PGA1_c00640 0.81 +1.2 65,617 + PGA1_c00640 0.81 +0.6 65,617 + PGA1_c00640 0.81 +0.4 65,617 + PGA1_c00640 0.81 -0.2 65,617 + PGA1_c00640 0.81 +0.6 65,617 + PGA1_c00640 0.81 +0.4 65,617 + PGA1_c00640 0.81 +0.1 65,633 + PGA1_c00640 0.81 +0.8 65,641 - PGA1_c00640 0.82 -0.5 65,680 + PGA1_c00640 0.84 +1.1 65,716 - PGA1_c00640 0.86 -0.1 65,763 + PGA1_c00640 0.88 -0.3 65,778 - PGA1_c00640 0.89 -0.4 65,799 + PGA1_c00640 0.90 -0.7 65,826 + -0.2 65,831 + +0.8 65,831 + -0.5 65,839 - +0.1 65,839 - +0.1 65,839 - -1.2 65,845 + -0.2 65,845 + -0.2 65,845 + +1.1 65,901 + -0.7 65,910 + -0.8 65,918 + -2.0 65,919 - +0.6 66,007 + -1.1 66,052 + -0.6 66,103 - +0.0 66,113 - +0.2 66,127 - +0.7 66,127 - +1.3 66,146 - +0.1 66,201 + PGA1_c00650 0.17 -1.0 66,202 - PGA1_c00650 0.17 +0.2 66,209 + PGA1_c00650 0.18 +0.2 66,220 - PGA1_c00650 0.20 +0.1 66,227 + PGA1_c00650 0.22 +0.3 66,235 - PGA1_c00650 0.23 -0.2 66,296 + PGA1_c00650 0.35 +1.2 66,296 + PGA1_c00650 0.35 +0.0 66,336 - PGA1_c00650 0.42 +1.7 66,338 + PGA1_c00650 0.43 +0.2 66,338 + PGA1_c00650 0.43 +2.6 66,338 + PGA1_c00650 0.43 -0.4 66,338 + PGA1_c00650 0.43 +0.8 66,338 + PGA1_c00650 0.43 -1.3 66,338 + PGA1_c00650 0.43 +0.4 66,338 + PGA1_c00650 0.43 +0.4 66,338 + PGA1_c00650 0.43 +0.3 66,338 + PGA1_c00650 0.43 +0.2 66,338 + PGA1_c00650 0.43 -1.1 66,338 + PGA1_c00650 0.43 -0.3 66,338 + PGA1_c00650 0.43 +1.0 66,338 + PGA1_c00650 0.43 +1.0 66,338 + PGA1_c00650 0.43 +0.4 66,338 + PGA1_c00650 0.43 +1.2 66,338 + PGA1_c00650 0.43 -2.8 66,346 - PGA1_c00650 0.44 -0.1 66,346 - PGA1_c00650 0.44 -0.7 66,346 - PGA1_c00650 0.44 +0.4 66,346 - PGA1_c00650 0.44 +1.1 66,346 - PGA1_c00650 0.44 +0.2 66,346 - PGA1_c00650 0.44 -0.9 66,391 - PGA1_c00650 0.53 +1.6 66,427 + PGA1_c00650 0.60 +0.4 66,442 - PGA1_c00650 0.62 +0.6 66,474 + PGA1_c00650 0.69 +0.5 66,518 - PGA1_c00650 0.77 -0.1 66,518 - PGA1_c00650 0.77 +1.6 66,523 - PGA1_c00650 0.78 +0.4 66,540 - PGA1_c00650 0.81 +0.2 66,569 + PGA1_c00650 0.87 -1.6 66,569 + PGA1_c00650 0.87 -2.1 66,574 + PGA1_c00650 0.88 +0.3 66,577 - PGA1_c00650 0.88 +0.3 66,582 - PGA1_c00650 0.89 +0.2 66,641 - -0.3 66,641 - +1.0 66,641 - +1.4 66,641 - -0.2 66,705 + +0.5 66,730 - -1.3 66,732 + -0.4 66,732 + -0.3 66,732 + -1.3 66,732 + -0.3 66,732 + +0.4 66,732 + -0.8 66,732 + +0.1 66,740 - -1.1 66,740 - -0.0 66,740 - -0.4 66,740 - -0.6 66,740 - +0.5 66,740 - +0.4 66,740 - +0.4 66,740 - +1.1 66,740 - +1.6 66,740 - -0.4 66,745 - -0.7 66,771 - -0.6 66,850 - PGA1_c00660 0.12 -0.3 66,871 - PGA1_c00660 0.14 +1.0 66,955 + PGA1_c00660 0.24 -1.2 66,955 + PGA1_c00660 0.24 -0.4 66,955 + PGA1_c00660 0.24 +0.4 66,955 + PGA1_c00660 0.24 -0.3 66,963 - PGA1_c00660 0.25 -0.1 66,963 - PGA1_c00660 0.25 +0.1 66,963 - PGA1_c00660 0.25 +0.3 66,963 - PGA1_c00660 0.25 -0.4 66,991 + PGA1_c00660 0.28 -0.1 66,998 + PGA1_c00660 0.29 +1.5 66,999 + PGA1_c00660 0.29 +0.2 67,102 - PGA1_c00660 0.41 +0.1 67,208 + PGA1_c00660 0.53 -0.6 67,238 + PGA1_c00660 0.56 -0.7 67,267 + PGA1_c00660 0.59 +0.4 67,284 - PGA1_c00660 0.61 +2.4 67,291 - PGA1_c00660 0.62 +1.2 67,329 - PGA1_c00660 0.66 -1.2 67,421 - PGA1_c00660 0.77 +0.9 67,429 + PGA1_c00660 0.78 -1.3 67,429 + PGA1_c00660 0.78 -0.3 67,437 - PGA1_c00660 0.79 +1.2 67,459 - PGA1_c00660 0.81 +0.9 67,487 - PGA1_c00660 0.84 -0.7 67,498 - PGA1_c00660 0.86 +0.7 67,531 - PGA1_c00660 0.89 +0.6 67,550 + -0.2 67,550 + -0.5 67,558 - +0.4 67,571 - +2.0 67,595 - +1.2 67,609 + -1.0 67,609 + -0.5 67,617 - +1.7 67,617 - -0.8
Or see this region's nucleotide sequence