Experiment: BHIS with Oxytetracycline 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0106 and BT0107 are separated by 449 nucleotides BT0107 and BT0108 are separated by 55 nucleotides BT0108 and BT0109 overlap by 38 nucleotides BT0109 and BT0110 are separated by 210 nucleotides
BT0106: BT0106 - conserved hypothetical protein (NCBI ptt file), at 99,073 to 99,519
BT0106
BT0107: BT0107 - conserved hypothetical protein (NCBI ptt file), at 99,969 to 100,247
BT0107
BT0108: BT0108 - conserved hypothetical protein (NCBI ptt file), at 100,303 to 102,303
BT0108
BT0109: BT0109 - conserved hypothetical protein (NCBI ptt file), at 102,266 to 102,625
BT0109
BT0110: BT0110 - putative regulatory protein (NCBI ptt file), at 102,836 to 103,696
BT0110
Position (kb)
100
101
102
103 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 99.333 kb on + strand, within BT0106 at 99.374 kb on + strand, within BT0106 at 99.399 kb on + strand, within BT0106 at 99.407 kb on + strand, within BT0106 at 99.443 kb on + strand, within BT0106 at 99.482 kb on + strand at 99.482 kb on + strand at 99.574 kb on - strand at 99.574 kb on - strand at 99.584 kb on - strand at 99.616 kb on + strand at 99.622 kb on + strand at 99.638 kb on - strand at 99.666 kb on - strand at 99.723 kb on - strand at 99.731 kb on - strand at 99.731 kb on - strand at 99.808 kb on - strand at 99.812 kb on + strand at 99.813 kb on - strand at 99.817 kb on - strand at 99.841 kb on + strand at 99.841 kb on + strand at 99.842 kb on - strand at 99.842 kb on - strand at 99.857 kb on + strand at 99.877 kb on - strand at 99.990 kb on + strand at 100.000 kb on - strand, within BT0107 at 100.015 kb on + strand, within BT0107 at 100.026 kb on - strand, within BT0107 at 100.051 kb on - strand, within BT0107 at 100.107 kb on + strand, within BT0107 at 100.108 kb on - strand, within BT0107 at 100.205 kb on + strand, within BT0107 at 100.243 kb on + strand at 100.244 kb on - strand at 100.247 kb on + strand at 100.248 kb on - strand at 100.254 kb on - strand at 100.267 kb on - strand at 100.282 kb on + strand at 100.353 kb on - strand at 100.399 kb on + strand at 100.588 kb on + strand, within BT0108 at 100.632 kb on + strand, within BT0108 at 100.747 kb on + strand, within BT0108 at 100.831 kb on + strand, within BT0108 at 100.857 kb on + strand, within BT0108 at 100.857 kb on + strand, within BT0108 at 100.923 kb on + strand, within BT0108 at 100.960 kb on + strand, within BT0108 at 100.972 kb on + strand, within BT0108 at 101.122 kb on - strand, within BT0108 at 101.200 kb on + strand, within BT0108 at 101.271 kb on + strand, within BT0108 at 101.275 kb on + strand, within BT0108 at 101.280 kb on + strand, within BT0108 at 101.462 kb on + strand, within BT0108 at 101.501 kb on + strand, within BT0108 at 101.626 kb on + strand, within BT0108 at 101.652 kb on + strand, within BT0108 at 101.652 kb on + strand, within BT0108 at 101.822 kb on + strand, within BT0108 at 101.822 kb on + strand, within BT0108 at 101.853 kb on - strand, within BT0108 at 101.864 kb on + strand, within BT0108 at 101.941 kb on + strand, within BT0108 at 101.943 kb on + strand, within BT0108 at 102.142 kb on + strand at 102.232 kb on - strand at 102.242 kb on + strand at 102.306 kb on - strand, within BT0109 at 102.526 kb on + strand, within BT0109 at 102.551 kb on + strand, within BT0109 at 102.617 kb on + strand at 102.728 kb on + strand at 102.839 kb on + strand at 102.889 kb on + strand at 102.923 kb on + strand, within BT0110 at 102.923 kb on + strand, within BT0110 at 102.924 kb on - strand, within BT0110 at 102.932 kb on - strand, within BT0110 at 102.943 kb on - strand, within BT0110 at 102.978 kb on - strand, within BT0110 at 102.982 kb on - strand, within BT0110 at 102.989 kb on - strand, within BT0110 at 103.000 kb on + strand, within BT0110 at 103.036 kb on - strand, within BT0110 at 103.056 kb on - strand, within BT0110 at 103.058 kb on + strand, within BT0110 at 103.065 kb on - strand, within BT0110 at 103.143 kb on + strand, within BT0110 at 103.144 kb on - strand, within BT0110 at 103.171 kb on + strand, within BT0110 at 103.212 kb on + strand, within BT0110
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Oxytetracycline 0.000390625 mM remove 99,333 + BT0106 0.58 -1.1 99,374 + BT0106 0.67 +1.1 99,399 + BT0106 0.73 +0.2 99,407 + BT0106 0.75 -0.4 99,443 + BT0106 0.83 +0.5 99,482 + +0.5 99,482 + -1.5 99,574 - -1.5 99,574 - -0.5 99,584 - +0.5 99,616 + -1.4 99,622 + +0.2 99,638 - +0.5 99,666 - -2.7 99,723 - -1.1 99,731 - -1.3 99,731 - -1.9 99,808 - -2.1 99,812 + -1.3 99,813 - -1.5 99,817 - -0.1 99,841 + -2.0 99,841 + -2.3 99,842 - +1.5 99,842 - +1.5 99,857 + +0.5 99,877 - -1.2 99,990 + +1.1 100,000 - BT0107 0.11 -1.5 100,015 + BT0107 0.16 -0.5 100,026 - BT0107 0.20 -2.1 100,051 - BT0107 0.29 -3.0 100,107 + BT0107 0.49 -0.9 100,108 - BT0107 0.50 +0.1 100,205 + BT0107 0.85 +0.5 100,243 + -0.5 100,244 - +1.5 100,247 + -1.1 100,248 - -1.1 100,254 - -0.5 100,267 - -2.1 100,282 + -1.1 100,353 - -1.9 100,399 + -0.1 100,588 + BT0108 0.14 -0.5 100,632 + BT0108 0.16 -2.3 100,747 + BT0108 0.22 -0.5 100,831 + BT0108 0.26 +0.5 100,857 + BT0108 0.28 -1.1 100,857 + BT0108 0.28 -2.3 100,923 + BT0108 0.31 +0.7 100,960 + BT0108 0.33 +0.5 100,972 + BT0108 0.33 +0.5 101,122 - BT0108 0.41 -1.1 101,200 + BT0108 0.45 +1.3 101,271 + BT0108 0.48 -0.5 101,275 + BT0108 0.49 -2.1 101,280 + BT0108 0.49 -1.5 101,462 + BT0108 0.58 +0.7 101,501 + BT0108 0.60 -1.5 101,626 + BT0108 0.66 +0.5 101,652 + BT0108 0.67 +0.8 101,652 + BT0108 0.67 +1.5 101,822 + BT0108 0.76 +0.5 101,822 + BT0108 0.76 +1.5 101,853 - BT0108 0.77 -1.9 101,864 + BT0108 0.78 -1.5 101,941 + BT0108 0.82 +0.5 101,943 + BT0108 0.82 +1.5 102,142 + -0.5 102,232 - -0.1 102,242 + +0.5 102,306 - BT0109 0.11 -1.3 102,526 + BT0109 0.72 -1.3 102,551 + BT0109 0.79 +1.5 102,617 + -0.9 102,728 + +0.5 102,839 + +0.5 102,889 + -0.5 102,923 + BT0110 0.10 -0.9 102,923 + BT0110 0.10 -1.1 102,924 - BT0110 0.10 +0.1 102,932 - BT0110 0.11 -0.5 102,943 - BT0110 0.12 +0.8 102,978 - BT0110 0.16 -0.4 102,982 - BT0110 0.17 -0.1 102,989 - BT0110 0.18 -2.1 103,000 + BT0110 0.19 -0.5 103,036 - BT0110 0.23 -2.5 103,056 - BT0110 0.26 -0.7 103,058 + BT0110 0.26 -1.3 103,065 - BT0110 0.27 +0.5 103,143 + BT0110 0.36 -0.6 103,144 - BT0110 0.36 -1.1 103,171 + BT0110 0.39 -0.5 103,212 + BT0110 0.44 -0.5
Or see this region's nucleotide sequence