Experiment: m.b. 1-ethyl-3-methylimidazolium lysine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06390 and PGA1_c06400 are separated by 100 nucleotides PGA1_c06400 and PGA1_c06410 are separated by 9 nucleotides PGA1_c06410 and PGA1_c06420 are separated by 207 nucleotides PGA1_c06420 and PGA1_c06430 are separated by 96 nucleotides
PGA1_c06390: PGA1_c06390 - putative sulfate transporter, at 654,214 to 655,854
_c06390
PGA1_c06400: PGA1_c06400 - putative antibiotic acetyltransferase, at 655,955 to 656,596
_c06400
PGA1_c06410: PGA1_c06410 - Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family, at 656,606 to 657,211
_c06410
PGA1_c06420: PGA1_c06420 - acetyltransferase domain-containing protein, at 657,419 to 657,928
_c06420
PGA1_c06430: PGA1_c06430 - hypothetical protein, at 658,025 to 658,804
_c06430
Position (kb) 656
657
658 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 655.637 kb on + strand, within PGA1_c06390 at 655.648 kb on - strand, within PGA1_c06390 at 655.700 kb on - strand at 655.750 kb on + strand at 655.776 kb on + strand at 655.815 kb on + strand at 655.815 kb on + strand at 655.856 kb on - strand at 655.858 kb on + strand at 655.866 kb on - strand at 655.891 kb on + strand at 655.956 kb on - strand at 656.038 kb on + strand, within PGA1_c06400 at 656.103 kb on + strand, within PGA1_c06400 at 656.103 kb on + strand, within PGA1_c06400 at 656.343 kb on + strand, within PGA1_c06400 at 656.354 kb on + strand, within PGA1_c06400 at 656.497 kb on - strand, within PGA1_c06400 at 656.529 kb on - strand, within PGA1_c06400 at 656.565 kb on - strand at 656.581 kb on + strand at 656.594 kb on - strand at 656.644 kb on + strand at 656.695 kb on + strand, within PGA1_c06410 at 656.761 kb on + strand, within PGA1_c06410 at 656.772 kb on + strand, within PGA1_c06410 at 656.772 kb on + strand, within PGA1_c06410 at 656.790 kb on - strand, within PGA1_c06410 at 656.941 kb on + strand, within PGA1_c06410 at 657.058 kb on + strand, within PGA1_c06410 at 657.058 kb on + strand, within PGA1_c06410 at 657.058 kb on + strand, within PGA1_c06410 at 657.058 kb on + strand, within PGA1_c06410 at 657.058 kb on + strand, within PGA1_c06410 at 657.066 kb on - strand, within PGA1_c06410 at 657.066 kb on - strand, within PGA1_c06410 at 657.193 kb on + strand at 657.201 kb on - strand at 657.273 kb on - strand at 657.299 kb on - strand at 657.299 kb on - strand at 657.301 kb on + strand at 657.309 kb on - strand at 657.309 kb on - strand at 657.372 kb on + strand at 657.411 kb on + strand at 657.441 kb on + strand at 657.468 kb on - strand at 657.521 kb on - strand, within PGA1_c06420 at 657.557 kb on - strand, within PGA1_c06420 at 657.641 kb on - strand, within PGA1_c06420 at 657.641 kb on - strand, within PGA1_c06420 at 657.646 kb on - strand, within PGA1_c06420 at 657.712 kb on - strand, within PGA1_c06420 at 657.714 kb on + strand, within PGA1_c06420 at 657.815 kb on - strand, within PGA1_c06420 at 657.857 kb on - strand, within PGA1_c06420 at 657.862 kb on + strand, within PGA1_c06420 at 657.876 kb on - strand, within PGA1_c06420 at 657.896 kb on - strand at 657.898 kb on + strand at 658.052 kb on + strand at 658.052 kb on + strand at 658.057 kb on + strand at 658.060 kb on - strand at 658.065 kb on - strand at 658.065 kb on - strand at 658.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium lysine 1 mM remove 655,637 + PGA1_c06390 0.87 +0.3 655,648 - PGA1_c06390 0.87 +0.9 655,700 - -0.2 655,750 + +1.4 655,776 + +0.0 655,815 + -1.3 655,815 + +0.6 655,856 - +0.5 655,858 + -0.3 655,866 - -1.0 655,891 + -0.6 655,956 - -0.3 656,038 + PGA1_c06400 0.13 -2.0 656,103 + PGA1_c06400 0.23 -0.3 656,103 + PGA1_c06400 0.23 +0.6 656,343 + PGA1_c06400 0.60 -0.5 656,354 + PGA1_c06400 0.62 -1.4 656,497 - PGA1_c06400 0.84 +0.9 656,529 - PGA1_c06400 0.89 -1.3 656,565 - -1.1 656,581 + -1.9 656,594 - -0.3 656,644 + +0.3 656,695 + PGA1_c06410 0.15 -0.3 656,761 + PGA1_c06410 0.26 -0.6 656,772 + PGA1_c06410 0.27 -0.8 656,772 + PGA1_c06410 0.27 -1.5 656,790 - PGA1_c06410 0.30 -0.1 656,941 + PGA1_c06410 0.55 +0.1 657,058 + PGA1_c06410 0.75 -1.0 657,058 + PGA1_c06410 0.75 -0.0 657,058 + PGA1_c06410 0.75 +0.0 657,058 + PGA1_c06410 0.75 -0.1 657,058 + PGA1_c06410 0.75 -0.9 657,066 - PGA1_c06410 0.76 -0.6 657,066 - PGA1_c06410 0.76 +0.5 657,193 + +0.6 657,201 - +1.6 657,273 - -3.3 657,299 - +0.6 657,299 - +0.3 657,301 + +0.7 657,309 - +0.7 657,309 - -0.2 657,372 + -1.9 657,411 + -0.8 657,441 + -1.6 657,468 - -2.2 657,521 - PGA1_c06420 0.20 -2.4 657,557 - PGA1_c06420 0.27 -2.0 657,641 - PGA1_c06420 0.44 -2.7 657,641 - PGA1_c06420 0.44 -2.3 657,646 - PGA1_c06420 0.45 -2.2 657,712 - PGA1_c06420 0.57 +0.3 657,714 + PGA1_c06420 0.58 -1.3 657,815 - PGA1_c06420 0.78 -1.8 657,857 - PGA1_c06420 0.86 -0.7 657,862 + PGA1_c06420 0.87 -3.2 657,876 - PGA1_c06420 0.90 -2.0 657,896 - -3.4 657,898 + -1.3 658,052 + -1.0 658,052 + -0.7 658,057 + -0.3 658,060 - +0.2 658,065 - +1.2 658,065 - +0.8 658,065 - -0.4
Or see this region's nucleotide sequence