Experiment: m.b. 1-ethyl-3-methylimidazolium lysine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c18500 and PGA1_c18510 are separated by 3 nucleotides PGA1_c18510 and PGA1_c18520 are separated by 9 nucleotides PGA1_c18520 and PGA1_c18530 are separated by 5 nucleotides PGA1_c18530 and PGA1_c18540 overlap by 1 nucleotides
PGA1_c18500: PGA1_c18500 - hypothetical protein, at 1,929,731 to 1,930,297
_c18500
PGA1_c18510: PGA1_c18510 - Protein of unknown function (DUF1064)., at 1,930,301 to 1,930,717
_c18510
PGA1_c18520: PGA1_c18520 - Uncharacterized protein conserved in bacteria, at 1,930,727 to 1,930,987
_c18520
PGA1_c18530: PGA1_c18530 - hypothetical protein, at 1,930,993 to 1,931,301
_c18530
PGA1_c18540: PGA1_c18540 - hypothetical protein, at 1,931,301 to 1,932,248
_c18540
Position (kb)
1930
1931 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1929.303 kb on + strand at 1929.303 kb on + strand at 1929.303 kb on + strand at 1929.303 kb on + strand at 1929.303 kb on + strand at 1929.303 kb on + strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.311 kb on - strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.331 kb on + strand at 1929.339 kb on - strand at 1929.339 kb on - strand at 1929.339 kb on - strand at 1929.339 kb on - strand at 1929.339 kb on - strand at 1929.341 kb on + strand at 1929.397 kb on - strand at 1929.397 kb on - strand at 1929.397 kb on - strand at 1929.397 kb on - strand at 1929.400 kb on + strand at 1929.400 kb on + strand at 1929.400 kb on + strand at 1929.402 kb on + strand at 1929.404 kb on - strand at 1929.404 kb on - strand at 1929.408 kb on - strand at 1929.408 kb on - strand at 1929.408 kb on - strand at 1929.408 kb on - strand at 1929.408 kb on - strand at 1929.410 kb on - strand at 1929.436 kb on + strand at 1929.436 kb on + strand at 1929.456 kb on + strand at 1929.489 kb on + strand at 1929.501 kb on - strand at 1929.519 kb on - strand at 1929.529 kb on + strand at 1929.537 kb on - strand at 1929.538 kb on - strand at 1929.543 kb on + strand at 1929.552 kb on + strand at 1929.552 kb on + strand at 1929.552 kb on + strand at 1929.552 kb on + strand at 1929.553 kb on + strand at 1929.556 kb on - strand at 1929.576 kb on - strand at 1929.578 kb on + strand at 1929.581 kb on + strand at 1929.583 kb on + strand at 1929.583 kb on + strand at 1929.589 kb on - strand at 1929.591 kb on - strand at 1929.598 kb on + strand at 1929.608 kb on + strand at 1929.609 kb on - strand at 1929.609 kb on - strand at 1929.619 kb on + strand at 1929.626 kb on + strand at 1929.626 kb on + strand at 1929.627 kb on - strand at 1929.627 kb on - strand at 1929.628 kb on - strand at 1929.634 kb on - strand at 1929.673 kb on + strand at 1929.689 kb on + strand at 1929.689 kb on - strand at 1929.691 kb on + strand at 1929.697 kb on - strand at 1929.701 kb on + strand at 1929.704 kb on - strand at 1929.716 kb on + strand at 1929.728 kb on + strand at 1929.755 kb on - strand at 1929.763 kb on + strand at 1929.763 kb on + strand at 1929.764 kb on + strand at 1929.772 kb on - strand at 1929.777 kb on + strand at 1929.783 kb on + strand at 1929.783 kb on + strand at 1929.783 kb on + strand at 1929.791 kb on - strand, within PGA1_c18500 at 1929.791 kb on - strand, within PGA1_c18500 at 1929.791 kb on - strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.795 kb on + strand, within PGA1_c18500 at 1929.796 kb on - strand, within PGA1_c18500 at 1929.802 kb on + strand, within PGA1_c18500 at 1929.802 kb on + strand, within PGA1_c18500 at 1929.810 kb on - strand, within PGA1_c18500 at 1929.825 kb on + strand, within PGA1_c18500 at 1929.865 kb on + strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.871 kb on - strand, within PGA1_c18500 at 1929.896 kb on - strand, within PGA1_c18500 at 1929.898 kb on + strand, within PGA1_c18500 at 1929.909 kb on + strand, within PGA1_c18500 at 1929.909 kb on + strand, within PGA1_c18500 at 1929.940 kb on - strand, within PGA1_c18500 at 1929.942 kb on - strand, within PGA1_c18500 at 1930.041 kb on + strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.041 kb on - strand, within PGA1_c18500 at 1930.098 kb on + strand, within PGA1_c18500 at 1930.098 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.106 kb on - strand, within PGA1_c18500 at 1930.110 kb on + strand, within PGA1_c18500 at 1930.124 kb on - strand, within PGA1_c18500 at 1930.161 kb on + strand, within PGA1_c18500 at 1930.161 kb on + strand, within PGA1_c18500 at 1930.169 kb on - strand, within PGA1_c18500 at 1930.173 kb on + strand, within PGA1_c18500 at 1930.174 kb on - strand, within PGA1_c18500 at 1930.210 kb on - strand, within PGA1_c18500 at 1930.211 kb on - strand, within PGA1_c18500 at 1930.225 kb on - strand, within PGA1_c18500 at 1930.230 kb on - strand, within PGA1_c18500 at 1930.267 kb on + strand at 1930.268 kb on - strand at 1930.272 kb on + strand at 1930.275 kb on - strand at 1930.275 kb on - strand at 1930.280 kb on - strand at 1930.334 kb on + strand at 1930.334 kb on + strand at 1930.342 kb on - strand at 1930.362 kb on + strand, within PGA1_c18510 at 1930.386 kb on + strand, within PGA1_c18510 at 1930.488 kb on - strand, within PGA1_c18510 at 1930.508 kb on + strand, within PGA1_c18510 at 1930.535 kb on + strand, within PGA1_c18510 at 1930.567 kb on + strand, within PGA1_c18510 at 1930.567 kb on + strand, within PGA1_c18510 at 1930.575 kb on - strand, within PGA1_c18510 at 1930.582 kb on + strand, within PGA1_c18510 at 1930.652 kb on + strand, within PGA1_c18510 at 1930.658 kb on + strand, within PGA1_c18510 at 1930.663 kb on + strand, within PGA1_c18510 at 1930.674 kb on - strand, within PGA1_c18510 at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.686 kb on + strand at 1930.694 kb on - strand at 1930.701 kb on + strand at 1930.718 kb on - strand at 1930.724 kb on + strand at 1930.732 kb on - strand at 1930.738 kb on - strand at 1930.828 kb on + strand, within PGA1_c18520 at 1930.844 kb on - strand, within PGA1_c18520 at 1930.860 kb on + strand, within PGA1_c18520 at 1930.860 kb on + strand, within PGA1_c18520 at 1930.902 kb on + strand, within PGA1_c18520 at 1930.910 kb on - strand, within PGA1_c18520 at 1930.911 kb on + strand, within PGA1_c18520 at 1930.920 kb on - strand, within PGA1_c18520 at 1930.941 kb on - strand, within PGA1_c18520 at 1930.962 kb on - strand at 1930.996 kb on + strand at 1930.996 kb on - strand at 1931.036 kb on - strand, within PGA1_c18530 at 1931.056 kb on + strand, within PGA1_c18530 at 1931.064 kb on - strand, within PGA1_c18530 at 1931.148 kb on + strand, within PGA1_c18530 at 1931.158 kb on + strand, within PGA1_c18530 at 1931.188 kb on + strand, within PGA1_c18530 at 1931.200 kb on - strand, within PGA1_c18530 at 1931.232 kb on - strand, within PGA1_c18530 at 1931.311 kb on + strand at 1931.319 kb on - strand at 1931.319 kb on - strand at 1931.378 kb on + strand at 1931.383 kb on + strand at 1931.409 kb on - strand, within PGA1_c18540 at 1931.415 kb on + strand, within PGA1_c18540 at 1931.415 kb on - strand, within PGA1_c18540 at 1931.433 kb on + strand, within PGA1_c18540 at 1931.441 kb on - strand, within PGA1_c18540 at 1931.441 kb on - strand, within PGA1_c18540 at 1931.470 kb on - strand, within PGA1_c18540 at 1931.498 kb on - strand, within PGA1_c18540 at 1931.519 kb on + strand, within PGA1_c18540 at 1931.537 kb on - strand, within PGA1_c18540 at 1931.557 kb on + strand, within PGA1_c18540 at 1931.557 kb on + strand, within PGA1_c18540 at 1931.565 kb on - strand, within PGA1_c18540 at 1931.568 kb on + strand, within PGA1_c18540 at 1931.612 kb on - strand, within PGA1_c18540 at 1931.612 kb on - strand, within PGA1_c18540 at 1931.636 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.665 kb on + strand, within PGA1_c18540 at 1931.670 kb on + strand, within PGA1_c18540
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium lysine 1 mM remove 1,929,303 + -0.0 1,929,303 + +0.2 1,929,303 + -2.5 1,929,303 + +1.0 1,929,303 + -0.4 1,929,303 + +0.3 1,929,311 - +0.5 1,929,311 - +0.6 1,929,311 - +0.3 1,929,311 - -0.3 1,929,311 - +1.4 1,929,311 - +0.8 1,929,311 - -0.1 1,929,311 - +0.2 1,929,311 - -0.6 1,929,311 - +0.9 1,929,311 - +1.2 1,929,311 - +1.3 1,929,311 - -0.0 1,929,331 + -0.5 1,929,331 + -1.1 1,929,331 + -0.5 1,929,331 + -1.0 1,929,331 + +0.7 1,929,331 + +0.3 1,929,331 + +0.0 1,929,331 + -0.0 1,929,331 + -0.6 1,929,339 - +0.6 1,929,339 - -1.0 1,929,339 - -2.9 1,929,339 - -0.4 1,929,339 - -1.1 1,929,341 + +0.4 1,929,397 - +1.2 1,929,397 - +0.2 1,929,397 - -0.1 1,929,397 - +1.2 1,929,400 + -0.3 1,929,400 + +1.1 1,929,400 + -0.4 1,929,402 + +0.2 1,929,404 - -0.1 1,929,404 - +0.4 1,929,408 - +0.7 1,929,408 - +1.7 1,929,408 - -0.6 1,929,408 - +0.9 1,929,408 - -0.3 1,929,410 - +0.5 1,929,436 + -0.6 1,929,436 + +0.6 1,929,456 + -1.0 1,929,489 + +0.2 1,929,501 - +0.5 1,929,519 - -1.1 1,929,529 + +0.6 1,929,537 - +1.4 1,929,538 - +0.5 1,929,543 + +0.4 1,929,552 + +0.2 1,929,552 + +0.9 1,929,552 + -0.5 1,929,552 + +1.8 1,929,553 + +0.5 1,929,556 - -2.7 1,929,576 - -0.7 1,929,578 + +1.0 1,929,581 + -0.1 1,929,583 + +0.3 1,929,583 + +0.3 1,929,589 - -1.6 1,929,591 - -0.6 1,929,598 + -0.0 1,929,608 + +0.5 1,929,609 - -0.8 1,929,609 - -1.7 1,929,619 + +0.1 1,929,626 + -2.0 1,929,626 + -0.6 1,929,627 - -1.8 1,929,627 - -0.3 1,929,628 - +0.3 1,929,634 - +0.7 1,929,673 + -1.1 1,929,689 + -1.2 1,929,689 - -0.1 1,929,691 + -0.7 1,929,697 - -0.2 1,929,701 + +1.2 1,929,704 - -0.3 1,929,716 + +0.0 1,929,728 + -0.2 1,929,755 - +0.3 1,929,763 + +0.4 1,929,763 + +0.6 1,929,764 + +0.6 1,929,772 - +1.6 1,929,777 + -0.3 1,929,783 + +1.2 1,929,783 + -1.0 1,929,783 + +0.0 1,929,791 - PGA1_c18500 0.11 +0.6 1,929,791 - PGA1_c18500 0.11 -1.1 1,929,791 - PGA1_c18500 0.11 +0.4 1,929,795 + PGA1_c18500 0.11 +0.6 1,929,795 + PGA1_c18500 0.11 -1.5 1,929,795 + PGA1_c18500 0.11 +0.8 1,929,796 - PGA1_c18500 0.11 -0.0 1,929,802 + PGA1_c18500 0.13 -1.8 1,929,802 + PGA1_c18500 0.13 -1.1 1,929,810 - PGA1_c18500 0.14 +0.7 1,929,825 + PGA1_c18500 0.17 +0.1 1,929,865 + PGA1_c18500 0.24 +0.4 1,929,871 - PGA1_c18500 0.25 -0.9 1,929,871 - PGA1_c18500 0.25 +0.9 1,929,871 - PGA1_c18500 0.25 +1.0 1,929,896 - PGA1_c18500 0.29 +0.3 1,929,898 + PGA1_c18500 0.29 +0.8 1,929,909 + PGA1_c18500 0.31 -0.3 1,929,909 + PGA1_c18500 0.31 -1.0 1,929,940 - PGA1_c18500 0.37 +0.4 1,929,942 - PGA1_c18500 0.37 +0.2 1,930,041 + PGA1_c18500 0.55 +0.5 1,930,041 - PGA1_c18500 0.55 -0.6 1,930,041 - PGA1_c18500 0.55 -0.9 1,930,041 - PGA1_c18500 0.55 -0.9 1,930,041 - PGA1_c18500 0.55 +0.6 1,930,098 + PGA1_c18500 0.65 -0.0 1,930,098 - PGA1_c18500 0.65 +0.1 1,930,106 - PGA1_c18500 0.66 -0.8 1,930,106 - PGA1_c18500 0.66 +0.9 1,930,106 - PGA1_c18500 0.66 -0.1 1,930,106 - PGA1_c18500 0.66 +0.5 1,930,110 + PGA1_c18500 0.67 -1.5 1,930,124 - PGA1_c18500 0.69 +1.0 1,930,161 + PGA1_c18500 0.76 -0.1 1,930,161 + PGA1_c18500 0.76 -0.2 1,930,169 - PGA1_c18500 0.77 +1.0 1,930,173 + PGA1_c18500 0.78 +1.5 1,930,174 - PGA1_c18500 0.78 +0.1 1,930,210 - PGA1_c18500 0.84 -1.3 1,930,211 - PGA1_c18500 0.85 -0.1 1,930,225 - PGA1_c18500 0.87 -0.1 1,930,230 - PGA1_c18500 0.88 +0.8 1,930,267 + -0.6 1,930,268 - +0.1 1,930,272 + -1.8 1,930,275 - +0.3 1,930,275 - +1.1 1,930,280 - +0.4 1,930,334 + -1.0 1,930,334 + -0.3 1,930,342 - +0.6 1,930,362 + PGA1_c18510 0.15 +1.1 1,930,386 + PGA1_c18510 0.20 +0.1 1,930,488 - PGA1_c18510 0.45 +0.0 1,930,508 + PGA1_c18510 0.50 +1.2 1,930,535 + PGA1_c18510 0.56 -2.4 1,930,567 + PGA1_c18510 0.64 +0.1 1,930,567 + PGA1_c18510 0.64 +1.2 1,930,575 - PGA1_c18510 0.66 -0.1 1,930,582 + PGA1_c18510 0.67 -0.4 1,930,652 + PGA1_c18510 0.84 +1.2 1,930,658 + PGA1_c18510 0.86 -0.1 1,930,663 + PGA1_c18510 0.87 +0.9 1,930,674 - PGA1_c18510 0.89 -0.7 1,930,686 + +0.1 1,930,686 + -0.1 1,930,686 + +0.2 1,930,686 + +0.1 1,930,694 - -1.6 1,930,701 + -0.1 1,930,718 - +0.4 1,930,724 + +0.6 1,930,732 - -0.0 1,930,738 - +1.7 1,930,828 + PGA1_c18520 0.39 +0.9 1,930,844 - PGA1_c18520 0.45 +0.9 1,930,860 + PGA1_c18520 0.51 -0.8 1,930,860 + PGA1_c18520 0.51 +0.2 1,930,902 + PGA1_c18520 0.67 +1.4 1,930,910 - PGA1_c18520 0.70 +0.4 1,930,911 + PGA1_c18520 0.70 +1.3 1,930,920 - PGA1_c18520 0.74 +0.1 1,930,941 - PGA1_c18520 0.82 +0.4 1,930,962 - +1.1 1,930,996 + +0.6 1,930,996 - -2.5 1,931,036 - PGA1_c18530 0.14 -0.3 1,931,056 + PGA1_c18530 0.20 -0.5 1,931,064 - PGA1_c18530 0.23 -0.4 1,931,148 + PGA1_c18530 0.50 +0.3 1,931,158 + PGA1_c18530 0.53 -0.6 1,931,188 + PGA1_c18530 0.63 +0.2 1,931,200 - PGA1_c18530 0.67 +0.2 1,931,232 - PGA1_c18530 0.77 -0.4 1,931,311 + +0.3 1,931,319 - +0.6 1,931,319 - +0.4 1,931,378 + +0.0 1,931,383 + +0.4 1,931,409 - PGA1_c18540 0.11 +0.2 1,931,415 + PGA1_c18540 0.12 +0.0 1,931,415 - PGA1_c18540 0.12 -0.1 1,931,433 + PGA1_c18540 0.14 -0.2 1,931,441 - PGA1_c18540 0.15 +0.8 1,931,441 - PGA1_c18540 0.15 -0.1 1,931,470 - PGA1_c18540 0.18 -0.3 1,931,498 - PGA1_c18540 0.21 -1.6 1,931,519 + PGA1_c18540 0.23 +0.6 1,931,537 - PGA1_c18540 0.25 +0.2 1,931,557 + PGA1_c18540 0.27 -0.0 1,931,557 + PGA1_c18540 0.27 +0.5 1,931,565 - PGA1_c18540 0.28 +0.3 1,931,568 + PGA1_c18540 0.28 -0.0 1,931,612 - PGA1_c18540 0.33 +0.8 1,931,612 - PGA1_c18540 0.33 +0.2 1,931,636 + PGA1_c18540 0.35 -0.0 1,931,665 + PGA1_c18540 0.38 +0.3 1,931,665 + PGA1_c18540 0.38 -0.5 1,931,665 + PGA1_c18540 0.38 +0.8 1,931,670 + PGA1_c18540 0.39 -0.7
Or see this region's nucleotide sequence