Experiment: m.b. 1-ethyl-3-methylimidazolium lysine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13400 and PGA1_c13410 are separated by 103 nucleotides PGA1_c13410 and PGA1_c13420 overlap by 4 nucleotides PGA1_c13420 and PGA1_c13430 are separated by 37 nucleotides
PGA1_c13400: PGA1_c13400 - phosphoribosylaminoimidazole-succinocarboxamide synthase PurC, at 1,384,381 to 1,385,142
_c13400
PGA1_c13410: PGA1_c13410 - phosphoribosylformylglycinamidine (FGAM) synthase PurS, at 1,385,246 to 1,385,479
_c13410
PGA1_c13420: PGA1_c13420 - phosphoribosylformylglycinamidine synthase 1, at 1,385,476 to 1,386,144
_c13420
PGA1_c13430: PGA1_c13430 - C4-dicarboxylate transport sensor protein DctB, at 1,386,182 to 1,387,951
_c13430
Position (kb)
1385
1386 Strain fitness (log2 ratio)
-2
-1
0
1 at 1384.251 kb on + strand at 1384.251 kb on + strand at 1384.259 kb on - strand at 1384.261 kb on + strand at 1384.293 kb on + strand at 1384.432 kb on + strand at 1384.813 kb on - strand, within PGA1_c13400 at 1384.838 kb on + strand, within PGA1_c13400 at 1384.968 kb on + strand, within PGA1_c13400 at 1384.971 kb on - strand, within PGA1_c13400 at 1384.982 kb on + strand, within PGA1_c13400 at 1384.990 kb on - strand, within PGA1_c13400 at 1385.008 kb on + strand, within PGA1_c13400 at 1385.076 kb on + strand at 1385.304 kb on - strand, within PGA1_c13410 at 1385.311 kb on - strand, within PGA1_c13410 at 1385.396 kb on + strand, within PGA1_c13410 at 1385.488 kb on + strand at 1385.496 kb on - strand at 1385.730 kb on + strand, within PGA1_c13420 at 1386.103 kb on + strand at 1386.126 kb on + strand at 1386.199 kb on + strand at 1386.390 kb on - strand, within PGA1_c13430
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium lysine 1 mM remove 1,384,251 + -0.7 1,384,251 + -0.6 1,384,259 - +0.9 1,384,261 + +0.2 1,384,293 + -0.1 1,384,432 + +0.3 1,384,813 - PGA1_c13400 0.57 +1.1 1,384,838 + PGA1_c13400 0.60 -0.4 1,384,968 + PGA1_c13400 0.77 -2.7 1,384,971 - PGA1_c13400 0.77 -1.0 1,384,982 + PGA1_c13400 0.79 -2.0 1,384,990 - PGA1_c13400 0.80 -2.2 1,385,008 + PGA1_c13400 0.82 -0.7 1,385,076 + +0.3 1,385,304 - PGA1_c13410 0.25 +1.5 1,385,311 - PGA1_c13410 0.28 -0.5 1,385,396 + PGA1_c13410 0.64 +0.5 1,385,488 + -0.8 1,385,496 - -1.4 1,385,730 + PGA1_c13420 0.38 +0.6 1,386,103 + +1.1 1,386,126 + -0.0 1,386,199 + -0.2 1,386,390 - PGA1_c13430 0.12 -0.5
Or see this region's nucleotide sequence