Experiment: m.b. acetate 140 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00280 and PGA1_262p00290 are separated by 200 nucleotides PGA1_262p00290 and PGA1_262p00300 are separated by 148 nucleotides PGA1_262p00300 and PGA1_262p00310 are separated by 67 nucleotides
PGA1_262p00280: PGA1_262p00280 - putative proline racemase, at 31,428 to 32,453
_262p00280
PGA1_262p00290: PGA1_262p00290 - putative transcriptional regulator, at 32,654 to 33,265
_262p00290
PGA1_262p00300: PGA1_262p00300 - ABC transporter, extracellular solute-binding protein, at 33,414 to 35,000
_262p00300
PGA1_262p00310: PGA1_262p00310 - glutathione transport system permease protein GsiC, at 35,068 to 36,009
_262p00310
Position (kb) 33
34
35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 32.460 kb on + strand at 32.613 kb on - strand at 32.623 kb on - strand at 32.623 kb on - strand at 32.623 kb on - strand at 32.667 kb on - strand at 32.712 kb on + strand at 32.712 kb on + strand at 32.720 kb on + strand, within PGA1_262p00290 at 32.720 kb on - strand, within PGA1_262p00290 at 32.720 kb on - strand, within PGA1_262p00290 at 32.780 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.049 kb on - strand, within PGA1_262p00290 at 33.122 kb on - strand, within PGA1_262p00290 at 33.151 kb on + strand, within PGA1_262p00290 at 33.159 kb on - strand, within PGA1_262p00290 at 33.159 kb on - strand, within PGA1_262p00290 at 33.172 kb on - strand, within PGA1_262p00290 at 33.174 kb on + strand, within PGA1_262p00290 at 33.174 kb on + strand, within PGA1_262p00290 at 33.237 kb on - strand at 33.259 kb on + strand at 33.278 kb on + strand at 33.286 kb on - strand at 33.305 kb on + strand at 33.399 kb on + strand at 33.456 kb on + strand at 33.464 kb on - strand at 33.464 kb on - strand at 33.490 kb on - strand at 33.646 kb on + strand, within PGA1_262p00300 at 33.955 kb on - strand, within PGA1_262p00300 at 34.198 kb on - strand, within PGA1_262p00300 at 34.258 kb on - strand, within PGA1_262p00300 at 34.383 kb on - strand, within PGA1_262p00300 at 34.457 kb on + strand, within PGA1_262p00300 at 34.488 kb on - strand, within PGA1_262p00300 at 34.504 kb on - strand, within PGA1_262p00300 at 34.923 kb on - strand at 34.955 kb on - strand at 35.105 kb on - strand at 35.288 kb on - strand, within PGA1_262p00310 at 35.290 kb on + strand, within PGA1_262p00310 at 35.296 kb on - strand, within PGA1_262p00310 at 35.298 kb on - strand, within PGA1_262p00310 at 35.298 kb on - strand, within PGA1_262p00310 at 35.307 kb on - strand, within PGA1_262p00310 at 35.453 kb on + strand, within PGA1_262p00310 at 35.474 kb on - strand, within PGA1_262p00310 at 35.624 kb on + strand, within PGA1_262p00310 at 35.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 140 mM remove 32,460 + +0.4 32,613 - +1.2 32,623 - -0.0 32,623 - -0.5 32,623 - -1.0 32,667 - +0.3 32,712 + -0.3 32,712 + +0.4 32,720 + PGA1_262p00290 0.11 -1.0 32,720 - PGA1_262p00290 0.11 +0.4 32,720 - PGA1_262p00290 0.11 +0.3 32,780 + PGA1_262p00290 0.21 -2.1 33,016 + PGA1_262p00290 0.59 -0.2 33,016 + PGA1_262p00290 0.59 +0.8 33,016 + PGA1_262p00290 0.59 +0.5 33,016 + PGA1_262p00290 0.59 -2.6 33,049 - PGA1_262p00290 0.65 +0.6 33,122 - PGA1_262p00290 0.76 +0.1 33,151 + PGA1_262p00290 0.81 +0.8 33,159 - PGA1_262p00290 0.83 -0.4 33,159 - PGA1_262p00290 0.83 -2.1 33,172 - PGA1_262p00290 0.85 +0.1 33,174 + PGA1_262p00290 0.85 +0.9 33,174 + PGA1_262p00290 0.85 -0.4 33,237 - -0.0 33,259 + -2.1 33,278 + -0.3 33,286 - -0.2 33,305 + +0.5 33,399 + -0.5 33,456 + -0.3 33,464 - -1.5 33,464 - -1.0 33,490 - +0.7 33,646 + PGA1_262p00300 0.15 +0.1 33,955 - PGA1_262p00300 0.34 +0.5 34,198 - PGA1_262p00300 0.49 +0.6 34,258 - PGA1_262p00300 0.53 +1.0 34,383 - PGA1_262p00300 0.61 +0.5 34,457 + PGA1_262p00300 0.66 +0.6 34,488 - PGA1_262p00300 0.68 +0.6 34,504 - PGA1_262p00300 0.69 -1.6 34,923 - -0.3 34,955 - -0.3 35,105 - -3.4 35,288 - PGA1_262p00310 0.23 -0.2 35,290 + PGA1_262p00310 0.24 +0.1 35,296 - PGA1_262p00310 0.24 +0.7 35,298 - PGA1_262p00310 0.24 -0.3 35,298 - PGA1_262p00310 0.24 +0.8 35,307 - PGA1_262p00310 0.25 -0.3 35,453 + PGA1_262p00310 0.41 +0.9 35,474 - PGA1_262p00310 0.43 +0.4 35,624 + PGA1_262p00310 0.59 +1.0 35,920 + -0.2
Or see this region's nucleotide sequence