Experiment: m.b. acetate 140 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11500 and PGA1_c11510 are separated by 169 nucleotides PGA1_c11510 and PGA1_c11520 are separated by 115 nucleotides
PGA1_c11500: PGA1_c11500 - putative pyridoxal-phosphate-dependent aminotransferase, at 1,188,450 to 1,189,670
_c11500
PGA1_c11510: PGA1_c11510 - gamma-glutamylputrescine oxidoreductase PuuB, at 1,189,840 to 1,191,150
_c11510
PGA1_c11520: PGA1_c11520 - gamma-glutamylputrescine synthetase PuuA, at 1,191,266 to 1,192,624
_c11520
Position (kb)
1189
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1 at 1188.879 kb on + strand, within PGA1_c11500 at 1188.887 kb on - strand, within PGA1_c11500 at 1188.889 kb on + strand, within PGA1_c11500 at 1189.079 kb on + strand, within PGA1_c11500 at 1189.243 kb on - strand, within PGA1_c11500 at 1189.251 kb on - strand, within PGA1_c11500 at 1189.325 kb on - strand, within PGA1_c11500 at 1189.341 kb on - strand, within PGA1_c11500 at 1189.392 kb on + strand, within PGA1_c11500 at 1189.478 kb on - strand, within PGA1_c11500 at 1189.555 kb on + strand at 1189.555 kb on + strand at 1189.563 kb on - strand at 1189.609 kb on + strand at 1189.741 kb on - strand at 1189.781 kb on + strand at 1189.800 kb on - strand at 1189.815 kb on + strand at 1189.874 kb on - strand at 1189.876 kb on - strand at 1189.900 kb on + strand at 1189.900 kb on + strand at 1189.923 kb on + strand at 1190.091 kb on + strand, within PGA1_c11510 at 1190.093 kb on - strand, within PGA1_c11510 at 1190.101 kb on - strand, within PGA1_c11510 at 1190.241 kb on + strand, within PGA1_c11510 at 1190.363 kb on + strand, within PGA1_c11510 at 1190.496 kb on - strand, within PGA1_c11510 at 1190.548 kb on + strand, within PGA1_c11510 at 1190.560 kb on + strand, within PGA1_c11510 at 1190.642 kb on + strand, within PGA1_c11510 at 1190.642 kb on + strand, within PGA1_c11510 at 1190.650 kb on - strand, within PGA1_c11510 at 1190.696 kb on + strand, within PGA1_c11510 at 1190.745 kb on - strand, within PGA1_c11510 at 1191.040 kb on + strand at 1191.040 kb on + strand at 1191.123 kb on + strand at 1191.157 kb on + strand at 1191.238 kb on - strand at 1191.283 kb on + strand at 1191.364 kb on + strand at 1191.371 kb on + strand at 1191.378 kb on + strand at 1191.398 kb on - strand at 1191.424 kb on - strand, within PGA1_c11520 at 1191.436 kb on + strand, within PGA1_c11520 at 1191.436 kb on - strand, within PGA1_c11520 at 1191.465 kb on + strand, within PGA1_c11520 at 1191.465 kb on + strand, within PGA1_c11520 at 1191.541 kb on + strand, within PGA1_c11520 at 1191.548 kb on - strand, within PGA1_c11520 at 1191.877 kb on - strand, within PGA1_c11520 at 1191.928 kb on + strand, within PGA1_c11520 at 1191.928 kb on - strand, within PGA1_c11520 at 1192.012 kb on + strand, within PGA1_c11520 at 1192.014 kb on - strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.042 kb on - strand, within PGA1_c11520 at 1192.101 kb on + strand, within PGA1_c11520
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 140 mM remove 1,188,879 + PGA1_c11500 0.35 +0.5 1,188,887 - PGA1_c11500 0.36 +1.4 1,188,889 + PGA1_c11500 0.36 -1.1 1,189,079 + PGA1_c11500 0.52 -2.4 1,189,243 - PGA1_c11500 0.65 -0.3 1,189,251 - PGA1_c11500 0.66 +0.3 1,189,325 - PGA1_c11500 0.72 +0.5 1,189,341 - PGA1_c11500 0.73 -0.7 1,189,392 + PGA1_c11500 0.77 -1.7 1,189,478 - PGA1_c11500 0.84 -2.5 1,189,555 + -0.4 1,189,555 + -0.3 1,189,563 - -0.2 1,189,609 + +1.0 1,189,741 - -0.5 1,189,781 + -0.4 1,189,800 - -0.5 1,189,815 + -1.0 1,189,874 - +0.1 1,189,876 - +0.7 1,189,900 + +0.2 1,189,900 + +0.2 1,189,923 + +0.6 1,190,091 + PGA1_c11510 0.19 +1.7 1,190,093 - PGA1_c11510 0.19 +1.5 1,190,101 - PGA1_c11510 0.20 -0.0 1,190,241 + PGA1_c11510 0.31 +0.1 1,190,363 + PGA1_c11510 0.40 -1.3 1,190,496 - PGA1_c11510 0.50 +0.5 1,190,548 + PGA1_c11510 0.54 +1.2 1,190,560 + PGA1_c11510 0.55 +0.2 1,190,642 + PGA1_c11510 0.61 +0.7 1,190,642 + PGA1_c11510 0.61 +0.1 1,190,650 - PGA1_c11510 0.62 +0.3 1,190,696 + PGA1_c11510 0.65 +0.4 1,190,745 - PGA1_c11510 0.69 +1.0 1,191,040 + -1.7 1,191,040 + -1.2 1,191,123 + +0.4 1,191,157 + -0.6 1,191,238 - -0.3 1,191,283 + +0.7 1,191,364 + -2.3 1,191,371 + -1.0 1,191,378 + +1.5 1,191,398 - -0.9 1,191,424 - PGA1_c11520 0.12 +0.1 1,191,436 + PGA1_c11520 0.13 +0.3 1,191,436 - PGA1_c11520 0.13 +1.1 1,191,465 + PGA1_c11520 0.15 +0.7 1,191,465 + PGA1_c11520 0.15 -1.4 1,191,541 + PGA1_c11520 0.20 -0.2 1,191,548 - PGA1_c11520 0.21 -0.9 1,191,877 - PGA1_c11520 0.45 -0.5 1,191,928 + PGA1_c11520 0.49 -2.0 1,191,928 - PGA1_c11520 0.49 +0.7 1,192,012 + PGA1_c11520 0.55 -1.4 1,192,014 - PGA1_c11520 0.55 -1.8 1,192,034 + PGA1_c11520 0.57 -2.0 1,192,034 + PGA1_c11520 0.57 -1.3 1,192,034 + PGA1_c11520 0.57 -0.0 1,192,034 + PGA1_c11520 0.57 -1.5 1,192,034 + PGA1_c11520 0.57 +0.7 1,192,042 - PGA1_c11520 0.57 +1.3 1,192,101 + PGA1_c11520 0.61 -0.0
Or see this region's nucleotide sequence