Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4530 and BT4531 are separated by 146 nucleotides BT4531 and BT4532 are separated by 68 nucleotides BT4532 and BT4533 are separated by 479 nucleotides
BT4530: BT4530 - ATP-dependent DNA helicase recG (NCBI ptt file), at 5,947,293 to 5,948,714
BT4530
BT4531: BT4531 - conserved hypothetical protein (NCBI ptt file), at 5,948,861 to 5,949,916
BT4531
BT4532: BT4532 - hypothetical protein (NCBI ptt file), at 5,949,985 to 5,950,302
BT4532
BT4533: BT4533 - conserved hypothetical protein (NCBI ptt file), at 5,950,782 to 5,952,131
BT4533
Position (kb)
5948
5949
5950 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5947.895 kb on + strand, within BT4530 at 5947.898 kb on - strand, within BT4530 at 5947.953 kb on - strand, within BT4530 at 5948.059 kb on - strand, within BT4530 at 5948.089 kb on - strand, within BT4530 at 5948.140 kb on + strand, within BT4530 at 5948.187 kb on - strand, within BT4530 at 5948.190 kb on - strand, within BT4530 at 5948.279 kb on + strand, within BT4530 at 5948.280 kb on - strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.365 kb on + strand, within BT4530 at 5948.373 kb on - strand, within BT4530 at 5948.433 kb on - strand, within BT4530 at 5948.728 kb on - strand at 5948.728 kb on - strand at 5948.728 kb on - strand at 5948.752 kb on - strand at 5948.757 kb on + strand at 5948.916 kb on - strand at 5948.916 kb on - strand at 5948.918 kb on - strand at 5948.921 kb on - strand at 5948.937 kb on - strand at 5948.937 kb on - strand at 5948.937 kb on - strand at 5948.963 kb on + strand at 5948.990 kb on - strand, within BT4531 at 5949.047 kb on + strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.076 kb on - strand, within BT4531 at 5949.150 kb on - strand, within BT4531 at 5949.158 kb on - strand, within BT4531 at 5949.158 kb on - strand, within BT4531 at 5949.160 kb on + strand, within BT4531 at 5949.211 kb on + strand, within BT4531 at 5949.239 kb on - strand, within BT4531 at 5949.311 kb on - strand, within BT4531 at 5949.347 kb on - strand, within BT4531 at 5949.461 kb on - strand, within BT4531 at 5949.481 kb on - strand, within BT4531 at 5949.481 kb on - strand, within BT4531 at 5949.513 kb on - strand, within BT4531 at 5949.527 kb on - strand, within BT4531 at 5949.706 kb on - strand, within BT4531 at 5949.726 kb on - strand, within BT4531 at 5949.728 kb on - strand, within BT4531 at 5949.853 kb on - strand at 5949.914 kb on + strand at 5949.921 kb on - strand at 5949.956 kb on + strand at 5949.957 kb on - strand at 5949.957 kb on - strand at 5949.960 kb on + strand at 5949.960 kb on + strand at 5949.961 kb on - strand at 5949.962 kb on + strand at 5949.972 kb on - strand at 5949.973 kb on + strand at 5950.010 kb on - strand at 5950.010 kb on - strand at 5950.015 kb on + strand at 5950.054 kb on - strand, within BT4532 at 5950.054 kb on - strand, within BT4532 at 5950.058 kb on - strand, within BT4532 at 5950.058 kb on - strand, within BT4532 at 5950.119 kb on - strand, within BT4532 at 5950.165 kb on - strand, within BT4532 at 5950.202 kb on + strand, within BT4532 at 5950.202 kb on + strand, within BT4532 at 5950.203 kb on - strand, within BT4532 at 5950.218 kb on - strand, within BT4532 at 5950.222 kb on - strand, within BT4532 at 5950.230 kb on - strand, within BT4532 at 5950.257 kb on + strand, within BT4532 at 5950.283 kb on - strand at 5950.283 kb on - strand at 5950.287 kb on + strand at 5950.330 kb on - strand at 5950.330 kb on - strand at 5950.332 kb on - strand at 5950.363 kb on + strand at 5950.363 kb on + strand at 5950.397 kb on + strand at 5950.397 kb on + strand at 5950.398 kb on - strand at 5950.439 kb on - strand at 5950.462 kb on + strand at 5950.463 kb on - strand at 5950.552 kb on + strand at 5950.552 kb on + strand at 5950.553 kb on - strand at 5950.588 kb on - strand at 5950.590 kb on + strand at 5950.609 kb on + strand at 5950.617 kb on + strand at 5950.617 kb on + strand at 5950.647 kb on - strand at 5950.716 kb on + strand at 5950.754 kb on - strand at 5950.781 kb on + strand at 5950.782 kb on - strand at 5950.786 kb on + strand at 5950.826 kb on - strand at 5950.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 5,947,895 + BT4530 0.42 -0.5 5,947,898 - BT4530 0.43 -0.9 5,947,953 - BT4530 0.46 -1.5 5,948,059 - BT4530 0.54 +1.1 5,948,089 - BT4530 0.56 +1.5 5,948,140 + BT4530 0.60 -0.9 5,948,187 - BT4530 0.63 +0.1 5,948,190 - BT4530 0.63 -0.4 5,948,279 + BT4530 0.69 -1.9 5,948,280 - BT4530 0.69 +2.2 5,948,334 + BT4530 0.73 +0.1 5,948,334 + BT4530 0.73 -3.1 5,948,334 + BT4530 0.73 +0.5 5,948,341 - BT4530 0.74 +1.4 5,948,341 - BT4530 0.74 +0.7 5,948,341 - BT4530 0.74 +1.4 5,948,365 + BT4530 0.75 -0.9 5,948,373 - BT4530 0.76 +0.9 5,948,433 - BT4530 0.80 +1.7 5,948,728 - -0.1 5,948,728 - -1.2 5,948,728 - -0.9 5,948,752 - +1.1 5,948,757 + +0.1 5,948,916 - +1.5 5,948,916 - +3.4 5,948,918 - +0.4 5,948,921 - +1.5 5,948,937 - +2.0 5,948,937 - +0.1 5,948,937 - +1.8 5,948,963 + -0.9 5,948,990 - BT4531 0.12 -1.5 5,949,047 + BT4531 0.18 +1.4 5,949,048 - BT4531 0.18 +1.8 5,949,048 - BT4531 0.18 +0.6 5,949,048 - BT4531 0.18 +2.0 5,949,076 - BT4531 0.20 +0.5 5,949,150 - BT4531 0.27 +0.9 5,949,158 - BT4531 0.28 +0.4 5,949,158 - BT4531 0.28 -2.3 5,949,160 + BT4531 0.28 +0.6 5,949,211 + BT4531 0.33 -0.4 5,949,239 - BT4531 0.36 +1.1 5,949,311 - BT4531 0.43 +0.5 5,949,347 - BT4531 0.46 +1.4 5,949,461 - BT4531 0.57 -0.9 5,949,481 - BT4531 0.59 +0.1 5,949,481 - BT4531 0.59 +2.9 5,949,513 - BT4531 0.62 +1.8 5,949,527 - BT4531 0.63 +0.3 5,949,706 - BT4531 0.80 -1.0 5,949,726 - BT4531 0.82 +0.6 5,949,728 - BT4531 0.82 +1.4 5,949,853 - +0.1 5,949,914 + +1.0 5,949,921 - +1.1 5,949,956 + +0.4 5,949,957 - +1.1 5,949,957 - +1.1 5,949,960 + +0.6 5,949,960 + +0.1 5,949,961 - +1.0 5,949,962 + -0.1 5,949,972 - -0.2 5,949,973 + +2.3 5,950,010 - +1.3 5,950,010 - +1.1 5,950,015 + -0.2 5,950,054 - BT4532 0.22 +0.8 5,950,054 - BT4532 0.22 -0.5 5,950,058 - BT4532 0.23 +0.6 5,950,058 - BT4532 0.23 +0.5 5,950,119 - BT4532 0.42 +1.4 5,950,165 - BT4532 0.57 +1.1 5,950,202 + BT4532 0.68 +0.3 5,950,202 + BT4532 0.68 +0.5 5,950,203 - BT4532 0.69 -0.9 5,950,218 - BT4532 0.73 +1.5 5,950,222 - BT4532 0.75 +1.6 5,950,230 - BT4532 0.77 +0.3 5,950,257 + BT4532 0.86 +1.1 5,950,283 - +1.9 5,950,283 - +0.6 5,950,287 + -1.2 5,950,330 - +0.9 5,950,330 - +0.1 5,950,332 - +1.6 5,950,363 + -0.5 5,950,363 + -2.7 5,950,397 + +0.3 5,950,397 + +1.6 5,950,398 - +0.1 5,950,439 - +2.1 5,950,462 + -1.3 5,950,463 - +1.1 5,950,552 + +1.1 5,950,552 + -1.3 5,950,553 - +3.1 5,950,588 - +1.2 5,950,590 + -0.3 5,950,609 + +0.1 5,950,617 + +1.1 5,950,617 + +1.5 5,950,647 - +1.5 5,950,716 + +0.2 5,950,754 - +0.1 5,950,781 + -0.9 5,950,782 - +0.7 5,950,786 + +0.5 5,950,826 - +1.0 5,950,872 - +0.8
Or see this region's nucleotide sequence