Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3864 and BT3865 are separated by 49 nucleotides BT3865 and BT3866 are separated by 168 nucleotides
BT3864: BT3864 - tryptophanyl-tRNA synthetase (NCBI ptt file), at 5,034,089 to 5,035,189
BT3864
BT3865: BT3865 - hypothetical protein (NCBI ptt file), at 5,035,239 to 5,035,451
BT3865
BT3866: BT3866 - ammonium-dependent carbamoyl phosphate synthetase CarB2 (EC 6.3.4.16) <small>(from data)</small>, at 5,035,620 to 5,038,841
BT3866
Position (kb) 5035
5036 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5035.263 kb on + strand, within BT3865 at 5035.264 kb on - strand, within BT3865 at 5035.274 kb on + strand, within BT3865 at 5035.274 kb on + strand, within BT3865 at 5035.281 kb on + strand, within BT3865 at 5035.281 kb on + strand, within BT3865 at 5035.282 kb on - strand, within BT3865 at 5035.392 kb on - strand, within BT3865 at 5035.524 kb on + strand at 5035.525 kb on - strand at 5035.736 kb on + strand at 5035.736 kb on + strand at 5035.799 kb on + strand at 5035.940 kb on + strand at 5035.945 kb on - strand, within BT3866 at 5035.958 kb on - strand, within BT3866 at 5035.980 kb on - strand, within BT3866 at 5035.986 kb on + strand, within BT3866 at 5035.987 kb on - strand, within BT3866 at 5035.987 kb on - strand, within BT3866 at 5036.116 kb on + strand, within BT3866 at 5036.116 kb on + strand, within BT3866 at 5036.300 kb on - strand, within BT3866 at 5036.348 kb on + strand, within BT3866 at 5036.420 kb on + strand, within BT3866 at 5036.420 kb on + strand, within BT3866 at 5036.450 kb on + strand, within BT3866
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 5,035,263 + BT3865 0.11 -1.3 5,035,264 - BT3865 0.12 -0.0 5,035,274 + BT3865 0.16 -1.0 5,035,274 + BT3865 0.16 -0.6 5,035,281 + BT3865 0.20 -0.0 5,035,281 + BT3865 0.20 -1.2 5,035,282 - BT3865 0.20 -1.0 5,035,392 - BT3865 0.72 +2.6 5,035,524 + -1.4 5,035,525 - -0.0 5,035,736 + -0.0 5,035,736 + +2.8 5,035,799 + +0.6 5,035,940 + +1.3 5,035,945 - BT3866 0.10 +1.1 5,035,958 - BT3866 0.10 +4.0 5,035,980 - BT3866 0.11 -1.6 5,035,986 + BT3866 0.11 -2.0 5,035,987 - BT3866 0.11 +2.6 5,035,987 - BT3866 0.11 -1.0 5,036,116 + BT3866 0.15 +2.4 5,036,116 + BT3866 0.15 -0.0 5,036,300 - BT3866 0.21 +3.6 5,036,348 + BT3866 0.23 +2.0 5,036,420 + BT3866 0.25 +2.2 5,036,420 + BT3866 0.25 -1.0 5,036,450 + BT3866 0.26 -0.4
Or see this region's nucleotide sequence