Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2233 and BT2234 are separated by 54 nucleotides BT2234 and BT2235 overlap by 1 nucleotides BT2235 and BT2236 are separated by 67 nucleotides BT2236 and BT2237 are separated by 50 nucleotides BT2237 and BT2238 are separated by 1 nucleotides
BT2233: BT2233 - hypothetical protein (NCBI ptt file), at 2,802,745 to 2,803,044
BT2233
BT2234: BT2234 - putative cytosine/adenosine deaminase (NCBI ptt file), at 2,803,099 to 2,803,542
BT2234
BT2235: BT2235 - hypothetical protein (NCBI ptt file), at 2,803,542 to 2,803,775
BT2235
BT2236: BT2236 - conserved hypothetical protein (NCBI ptt file), at 2,803,843 to 2,804,208
BT2236
BT2237: BT2237 - conserved hypothetical protein (NCBI ptt file), at 2,804,259 to 2,804,615
BT2237
BT2238: BT2238 - putative biotin--(acetyl-CoA carboxylase) synthetase (NCBI ptt file), at 2,804,617 to 2,805,363
BT2238
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2803.045 kb on + strand at 2803.047 kb on + strand at 2803.052 kb on + strand at 2803.085 kb on + strand at 2803.641 kb on - strand, within BT2235 at 2803.733 kb on - strand, within BT2235 at 2803.776 kb on - strand at 2803.797 kb on - strand at 2804.013 kb on + strand, within BT2236 at 2804.099 kb on + strand, within BT2236 at 2804.168 kb on + strand, within BT2236 at 2804.177 kb on + strand at 2804.191 kb on + strand at 2804.201 kb on + strand at 2804.258 kb on + strand at 2804.290 kb on + strand at 2804.290 kb on + strand at 2804.332 kb on + strand, within BT2237 at 2804.445 kb on + strand, within BT2237 at 2804.515 kb on + strand, within BT2237
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 2,803,045 + +1.6 2,803,047 + -2.0 2,803,052 + -2.3 2,803,085 + -2.0 2,803,641 - BT2235 0.42 -2.3 2,803,733 - BT2235 0.82 -2.4 2,803,776 - -0.6 2,803,797 - -1.6 2,804,013 + BT2236 0.46 -4.1 2,804,099 + BT2236 0.70 -3.0 2,804,168 + BT2236 0.89 -1.1 2,804,177 + +0.0 2,804,191 + +0.0 2,804,201 + -0.7 2,804,258 + -1.6 2,804,290 + -1.6 2,804,290 + +0.0 2,804,332 + BT2237 0.20 -1.0 2,804,445 + BT2237 0.52 -1.8 2,804,515 + BT2237 0.72 -0.7
Or see this region's nucleotide sequence