Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1579 and BT1580 are separated by 13 nucleotides BT1580 and BT1581 are separated by 59 nucleotides BT1581 and BT1582 are separated by 9 nucleotides BT1582 and BT1583 are separated by 159 nucleotides BT1583 and tRNA-Thr are separated by 101 nucleotides
BT1579: BT1579 - hypothetical protein (NCBI ptt file), at 1,942,827 to 1,943,960
BT1579
BT1580: BT1580 - lactoylglutathione lyase (NCBI ptt file), at 1,943,974 to 1,944,354
BT1580
BT1581: BT1581 - hypothetical protein (NCBI ptt file), at 1,944,414 to 1,944,866
BT1581
BT1582: BT1582 - putative methyltransferase (NCBI ptt file), at 1,944,876 to 1,945,883
BT1582
BT1583: BT1583 - putative protein disulfide isomerase (NCBI ptt file), at 1,946,043 to 1,946,645
BT1583
BT_t27: tRNA-Thr - tRNA-Thr (RefSeq), at 1,946,747 to 1,946,820
tRNA-Thr
Position (kb)
1944
1945
1946 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1943.922 kb on + strand at 1943.944 kb on + strand at 1943.945 kb on - strand at 1943.958 kb on + strand at 1943.966 kb on + strand at 1943.966 kb on + strand at 1944.003 kb on + strand at 1944.073 kb on - strand, within BT1580 at 1944.073 kb on - strand, within BT1580 at 1944.113 kb on - strand, within BT1580 at 1944.206 kb on + strand, within BT1580 at 1944.247 kb on + strand, within BT1580 at 1944.335 kb on + strand at 1944.335 kb on + strand at 1944.336 kb on - strand at 1944.336 kb on - strand at 1944.419 kb on + strand at 1944.430 kb on + strand at 1944.430 kb on + strand at 1944.430 kb on + strand at 1944.430 kb on + strand at 1944.431 kb on - strand at 1944.476 kb on + strand, within BT1581 at 1944.476 kb on + strand, within BT1581 at 1944.477 kb on - strand, within BT1581 at 1944.481 kb on - strand, within BT1581 at 1944.545 kb on + strand, within BT1581 at 1944.608 kb on + strand, within BT1581 at 1944.671 kb on + strand, within BT1581 at 1944.719 kb on + strand, within BT1581 at 1944.720 kb on - strand, within BT1581 at 1944.744 kb on + strand, within BT1581 at 1944.745 kb on - strand, within BT1581 at 1944.781 kb on - strand, within BT1581 at 1944.803 kb on + strand, within BT1581 at 1944.844 kb on - strand at 1944.947 kb on + strand at 1944.949 kb on + strand at 1944.950 kb on - strand at 1944.998 kb on + strand, within BT1582 at 1945.007 kb on + strand, within BT1582 at 1945.013 kb on + strand, within BT1582 at 1945.044 kb on + strand, within BT1582 at 1945.045 kb on - strand, within BT1582 at 1945.045 kb on - strand, within BT1582 at 1945.047 kb on - strand, within BT1582 at 1945.052 kb on + strand, within BT1582 at 1945.052 kb on + strand, within BT1582 at 1945.053 kb on - strand, within BT1582 at 1945.053 kb on - strand, within BT1582 at 1945.054 kb on + strand, within BT1582 at 1945.055 kb on - strand, within BT1582 at 1945.055 kb on - strand, within BT1582 at 1945.059 kb on + strand, within BT1582 at 1945.059 kb on + strand, within BT1582 at 1945.060 kb on - strand, within BT1582 at 1945.117 kb on - strand, within BT1582 at 1945.141 kb on + strand, within BT1582 at 1945.142 kb on - strand, within BT1582 at 1945.358 kb on - strand, within BT1582 at 1945.364 kb on - strand, within BT1582 at 1945.521 kb on + strand, within BT1582 at 1945.561 kb on + strand, within BT1582 at 1945.561 kb on + strand, within BT1582 at 1945.561 kb on + strand, within BT1582 at 1945.562 kb on - strand, within BT1582 at 1945.571 kb on - strand, within BT1582 at 1945.575 kb on - strand, within BT1582 at 1945.607 kb on + strand, within BT1582 at 1945.612 kb on + strand, within BT1582 at 1945.628 kb on + strand, within BT1582 at 1945.680 kb on - strand, within BT1582 at 1945.757 kb on + strand, within BT1582 at 1945.757 kb on + strand, within BT1582 at 1945.772 kb on - strand, within BT1582 at 1945.774 kb on - strand, within BT1582 at 1945.774 kb on - strand, within BT1582 at 1945.793 kb on + strand at 1945.793 kb on + strand at 1945.800 kb on + strand at 1945.932 kb on - strand at 1945.944 kb on + strand at 1945.958 kb on + strand at 1945.959 kb on - strand at 1945.959 kb on - strand at 1945.964 kb on + strand at 1945.966 kb on + strand at 1945.984 kb on - strand at 1946.000 kb on - strand at 1946.003 kb on + strand at 1946.044 kb on + strand at 1946.136 kb on + strand, within BT1583 at 1946.310 kb on - strand, within BT1583 at 1946.359 kb on - strand, within BT1583 at 1946.485 kb on + strand, within BT1583 at 1946.485 kb on + strand, within BT1583 at 1946.592 kb on + strand at 1946.598 kb on - strand at 1946.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 1,943,922 + -0.7 1,943,944 + +0.0 1,943,945 - -2.7 1,943,958 + +0.0 1,943,966 + +0.0 1,943,966 + +0.6 1,944,003 + -2.9 1,944,073 - BT1580 0.26 -2.2 1,944,073 - BT1580 0.26 +0.5 1,944,113 - BT1580 0.36 -1.0 1,944,206 + BT1580 0.61 +0.6 1,944,247 + BT1580 0.72 +0.0 1,944,335 + -1.0 1,944,335 + -2.3 1,944,336 - -3.1 1,944,336 - -1.0 1,944,419 + -2.6 1,944,430 + -1.0 1,944,430 + -2.0 1,944,430 + +0.0 1,944,430 + -1.5 1,944,431 - -0.5 1,944,476 + BT1581 0.14 +1.0 1,944,476 + BT1581 0.14 -0.9 1,944,477 - BT1581 0.14 -1.2 1,944,481 - BT1581 0.15 +0.0 1,944,545 + BT1581 0.29 -0.4 1,944,608 + BT1581 0.43 -2.0 1,944,671 + BT1581 0.57 +0.0 1,944,719 + BT1581 0.67 -2.1 1,944,720 - BT1581 0.68 -3.3 1,944,744 + BT1581 0.73 +0.0 1,944,745 - BT1581 0.73 -2.3 1,944,781 - BT1581 0.81 -2.0 1,944,803 + BT1581 0.86 -1.0 1,944,844 - -0.4 1,944,947 + -1.8 1,944,949 + -2.8 1,944,950 - -1.5 1,944,998 + BT1582 0.12 -3.0 1,945,007 + BT1582 0.13 -2.5 1,945,013 + BT1582 0.14 -1.5 1,945,044 + BT1582 0.17 -2.1 1,945,045 - BT1582 0.17 -1.5 1,945,045 - BT1582 0.17 -2.1 1,945,047 - BT1582 0.17 -1.0 1,945,052 + BT1582 0.17 -1.3 1,945,052 + BT1582 0.17 -2.2 1,945,053 - BT1582 0.18 -4.5 1,945,053 - BT1582 0.18 -1.1 1,945,054 + BT1582 0.18 +0.0 1,945,055 - BT1582 0.18 -2.8 1,945,055 - BT1582 0.18 +0.0 1,945,059 + BT1582 0.18 -2.5 1,945,059 + BT1582 0.18 -2.2 1,945,060 - BT1582 0.18 -1.5 1,945,117 - BT1582 0.24 -0.5 1,945,141 + BT1582 0.26 -1.0 1,945,142 - BT1582 0.26 -2.7 1,945,358 - BT1582 0.48 -2.3 1,945,364 - BT1582 0.48 -2.0 1,945,521 + BT1582 0.64 -2.0 1,945,561 + BT1582 0.68 -1.8 1,945,561 + BT1582 0.68 -1.3 1,945,561 + BT1582 0.68 -0.3 1,945,562 - BT1582 0.68 -1.5 1,945,571 - BT1582 0.69 -1.0 1,945,575 - BT1582 0.69 +0.0 1,945,607 + BT1582 0.73 -1.5 1,945,612 + BT1582 0.73 -2.0 1,945,628 + BT1582 0.75 -3.0 1,945,680 - BT1582 0.80 -1.7 1,945,757 + BT1582 0.87 -2.3 1,945,757 + BT1582 0.87 +0.0 1,945,772 - BT1582 0.89 -1.0 1,945,774 - BT1582 0.89 +1.6 1,945,774 - BT1582 0.89 -1.5 1,945,793 + -1.0 1,945,793 + -2.3 1,945,800 + -1.3 1,945,932 - -1.6 1,945,944 + -2.5 1,945,958 + +1.6 1,945,959 - -2.3 1,945,959 - -0.5 1,945,964 + -2.0 1,945,966 + +0.0 1,945,984 - -3.9 1,946,000 - +0.6 1,946,003 + -1.6 1,946,044 + -2.7 1,946,136 + BT1583 0.15 +1.6 1,946,310 - BT1583 0.44 -1.5 1,946,359 - BT1583 0.52 -1.0 1,946,485 + BT1583 0.73 +0.0 1,946,485 + BT1583 0.73 +0.0 1,946,592 + -2.0 1,946,598 - -1.5 1,946,721 - -1.0
Or see this region's nucleotide sequence