Experiment: BG11 with Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1248 and psaJ are separated by 497 nucleotides psaJ and psaF are separated by 25 nucleotides psaF and Synpcc7942_1251 are separated by 177 nucleotides
Synpcc7942_1248: Synpcc7942_1248 - hypothetical protein, at 1,272,551 to 1,273,123
_1248
Synpcc7942_1249: psaJ - photosystem I reaction center subunit IX, at 1,273,621 to 1,273,755
psaJ
Synpcc7942_1250: psaF - photosystem I reaction center subunit III precursor, at 1,273,781 to 1,274,260
psaF
Synpcc7942_1251: Synpcc7942_1251 - O-sialoglycoprotein endopeptidase, at 1,274,438 to 1,275,487
_1251
Position (kb)
1273
1274
1275 Strain fitness (log2 ratio)
-2
-1
0
1 at 1272.786 kb on + strand, within Synpcc7942_1248 at 1272.794 kb on - strand, within Synpcc7942_1248 at 1272.806 kb on - strand, within Synpcc7942_1248 at 1272.846 kb on + strand, within Synpcc7942_1248 at 1272.859 kb on + strand, within Synpcc7942_1248 at 1272.864 kb on + strand, within Synpcc7942_1248 at 1272.864 kb on + strand, within Synpcc7942_1248 at 1272.864 kb on + strand, within Synpcc7942_1248 at 1272.874 kb on + strand, within Synpcc7942_1248 at 1272.882 kb on - strand, within Synpcc7942_1248 at 1272.886 kb on - strand, within Synpcc7942_1248 at 1272.908 kb on + strand at 1272.908 kb on + strand, within Synpcc7942_1248 at 1272.908 kb on + strand, within Synpcc7942_1248 at 1272.908 kb on + strand, within Synpcc7942_1248 at 1272.915 kb on - strand, within Synpcc7942_1248 at 1272.916 kb on - strand, within Synpcc7942_1248 at 1272.916 kb on - strand, within Synpcc7942_1248 at 1273.016 kb on - strand, within Synpcc7942_1248 at 1273.017 kb on - strand, within Synpcc7942_1248 at 1273.041 kb on + strand, within Synpcc7942_1248 at 1273.078 kb on + strand at 1273.101 kb on + strand at 1273.101 kb on + strand at 1273.146 kb on + strand at 1273.229 kb on + strand at 1273.229 kb on + strand at 1273.237 kb on - strand at 1273.237 kb on - strand at 1273.237 kb on - strand at 1273.273 kb on - strand at 1273.286 kb on - strand at 1273.295 kb on + strand at 1273.334 kb on + strand at 1273.418 kb on - strand at 1273.469 kb on + strand at 1273.587 kb on - strand at 1273.962 kb on - strand, within psaF at 1274.169 kb on - strand, within psaF at 1274.193 kb on + strand, within psaF at 1274.218 kb on + strand at 1274.271 kb on + strand at 1274.285 kb on + strand at 1274.285 kb on + strand at 1274.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.0625 mg/ml remove 1,272,786 + Synpcc7942_1248 0.41 -1.1 1,272,794 - Synpcc7942_1248 0.42 -0.0 1,272,806 - Synpcc7942_1248 0.45 -0.8 1,272,846 + Synpcc7942_1248 0.51 -0.8 1,272,859 + Synpcc7942_1248 0.54 -0.7 1,272,864 + Synpcc7942_1248 0.55 +0.2 1,272,864 + Synpcc7942_1248 0.55 +0.2 1,272,864 + Synpcc7942_1248 0.55 +0.1 1,272,874 + Synpcc7942_1248 0.56 +1.1 1,272,882 - Synpcc7942_1248 0.58 +0.1 1,272,886 - Synpcc7942_1248 0.58 -0.3 1,272,908 + +1.3 1,272,908 + Synpcc7942_1248 0.62 +0.2 1,272,908 + Synpcc7942_1248 0.62 -1.2 1,272,908 + Synpcc7942_1248 0.62 -2.6 1,272,915 - Synpcc7942_1248 0.64 -1.4 1,272,916 - Synpcc7942_1248 0.64 -0.5 1,272,916 - Synpcc7942_1248 0.64 -0.5 1,273,016 - Synpcc7942_1248 0.81 -1.2 1,273,017 - Synpcc7942_1248 0.81 +1.1 1,273,041 + Synpcc7942_1248 0.86 -0.7 1,273,078 + +1.3 1,273,101 + -2.3 1,273,101 + -1.1 1,273,146 + -1.5 1,273,229 + -0.8 1,273,229 + +0.6 1,273,237 - -1.8 1,273,237 - -1.4 1,273,237 - -0.8 1,273,273 - -1.7 1,273,286 - +0.5 1,273,295 + +0.7 1,273,334 + +0.3 1,273,418 - +0.9 1,273,469 + -1.3 1,273,587 - +1.6 1,273,962 - psaF Synpcc7942_1250 0.38 -1.2 1,274,169 - psaF Synpcc7942_1250 0.81 -0.7 1,274,193 + psaF Synpcc7942_1250 0.86 -2.5 1,274,218 + -0.7 1,274,271 + -1.1 1,274,285 + -0.4 1,274,285 + +0.9 1,274,293 - -0.9
Or see this region's nucleotide sequence