Experiment: m.b. acetate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00300 and PGA1_65p00310 are separated by 4 nucleotides PGA1_65p00310 and PGA1_65p00320 are separated by 190 nucleotides
PGA1_65p00300: PGA1_65p00300 - GDP-L-fucose synthetase Fcl, at 39,409 to 40,398
_65p00300
PGA1_65p00310: PGA1_65p00310 - GDP-mannose 4,6-dehydratase Gmd, at 40,403 to 41,521
_65p00310
PGA1_65p00320: PGA1_65p00320 - type I secretion membrane fusion protein, at 41,712 to 43,019
_65p00320
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1
2 at 39.457 kb on + strand at 39.465 kb on - strand at 39.586 kb on + strand, within PGA1_65p00300 at 39.594 kb on - strand, within PGA1_65p00300 at 39.939 kb on + strand, within PGA1_65p00300 at 39.979 kb on - strand, within PGA1_65p00300 at 40.103 kb on + strand, within PGA1_65p00300 at 40.343 kb on - strand at 40.501 kb on + strand at 40.804 kb on + strand, within PGA1_65p00310 at 40.883 kb on + strand, within PGA1_65p00310 at 40.893 kb on - strand, within PGA1_65p00310 at 40.901 kb on - strand, within PGA1_65p00310 at 41.119 kb on + strand, within PGA1_65p00310 at 41.119 kb on + strand, within PGA1_65p00310 at 41.322 kb on - strand, within PGA1_65p00310 at 41.537 kb on - strand at 41.556 kb on + strand at 41.556 kb on + strand at 41.564 kb on - strand at 41.564 kb on - strand at 41.585 kb on - strand at 41.600 kb on + strand at 41.889 kb on - strand, within PGA1_65p00320 at 41.977 kb on - strand, within PGA1_65p00320
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 100 mM remove 39,457 + +0.8 39,465 - -0.3 39,586 + PGA1_65p00300 0.18 +0.6 39,594 - PGA1_65p00300 0.19 +0.8 39,939 + PGA1_65p00300 0.54 +1.4 39,979 - PGA1_65p00300 0.58 -0.9 40,103 + PGA1_65p00300 0.70 -0.2 40,343 - +1.2 40,501 + -0.3 40,804 + PGA1_65p00310 0.36 -0.2 40,883 + PGA1_65p00310 0.43 -0.2 40,893 - PGA1_65p00310 0.44 +2.2 40,901 - PGA1_65p00310 0.45 +0.9 41,119 + PGA1_65p00310 0.64 -0.5 41,119 + PGA1_65p00310 0.64 -0.0 41,322 - PGA1_65p00310 0.82 +1.1 41,537 - +1.4 41,556 + +0.4 41,556 + +0.2 41,564 - +0.0 41,564 - -1.0 41,585 - -2.3 41,600 + -0.8 41,889 - PGA1_65p00320 0.14 -1.5 41,977 - PGA1_65p00320 0.20 +1.1
Or see this region's nucleotide sequence