Experiment: m.b. acetate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01370 and PGA1_262p01380 overlap by 4 nucleotides PGA1_262p01380 and PGA1_262p01390 are separated by 3 nucleotides PGA1_262p01390 and PGA1_262p01400 are separated by 144 nucleotides
PGA1_262p01370: PGA1_262p01370 - putative dioxygenase, at 147,465 to 148,619
_262p01370
PGA1_262p01380: PGA1_262p01380 - aldehyde dehydrogenase, at 148,616 to 150,067
_262p01380
PGA1_262p01390: PGA1_262p01390 - hypothetical protein, at 150,071 to 150,310
_262p01390
PGA1_262p01400: PGA1_262p01400 - dimethylglycine dehydrogenase DmgdH, at 150,455 to 152,875
_262p01400
Position (kb) 148
149
150
151 Strain fitness (log2 ratio) -2
-1
0
1 at 147.628 kb on - strand, within PGA1_262p01370 at 147.690 kb on + strand, within PGA1_262p01370 at 147.712 kb on + strand, within PGA1_262p01370 at 147.778 kb on + strand, within PGA1_262p01370 at 147.786 kb on - strand, within PGA1_262p01370 at 147.846 kb on + strand, within PGA1_262p01370 at 147.859 kb on - strand, within PGA1_262p01370 at 147.893 kb on - strand, within PGA1_262p01370 at 148.068 kb on - strand, within PGA1_262p01370 at 148.111 kb on - strand, within PGA1_262p01370 at 148.119 kb on + strand, within PGA1_262p01370 at 148.121 kb on - strand, within PGA1_262p01370 at 148.177 kb on + strand, within PGA1_262p01370 at 148.192 kb on + strand, within PGA1_262p01370 at 148.304 kb on + strand, within PGA1_262p01370 at 148.379 kb on - strand, within PGA1_262p01370 at 148.441 kb on - strand, within PGA1_262p01370 at 148.578 kb on - strand at 148.643 kb on + strand at 148.765 kb on - strand, within PGA1_262p01380 at 148.775 kb on - strand, within PGA1_262p01380 at 148.791 kb on + strand, within PGA1_262p01380 at 149.019 kb on + strand, within PGA1_262p01380 at 149.019 kb on + strand, within PGA1_262p01380 at 149.103 kb on - strand, within PGA1_262p01380 at 149.117 kb on + strand, within PGA1_262p01380 at 149.124 kb on - strand, within PGA1_262p01380 at 149.125 kb on - strand, within PGA1_262p01380 at 149.471 kb on + strand, within PGA1_262p01380 at 149.536 kb on - strand, within PGA1_262p01380 at 149.536 kb on - strand, within PGA1_262p01380 at 149.726 kb on + strand, within PGA1_262p01380 at 149.726 kb on + strand, within PGA1_262p01380 at 149.918 kb on + strand, within PGA1_262p01380 at 149.926 kb on - strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.027 kb on - strand at 150.027 kb on - strand at 150.066 kb on + strand at 150.092 kb on - strand at 150.134 kb on - strand, within PGA1_262p01390 at 150.150 kb on - strand, within PGA1_262p01390 at 150.267 kb on - strand, within PGA1_262p01390 at 150.278 kb on + strand, within PGA1_262p01390 at 150.286 kb on - strand, within PGA1_262p01390 at 150.286 kb on - strand, within PGA1_262p01390 at 150.358 kb on + strand at 150.366 kb on - strand at 150.447 kb on + strand at 150.470 kb on - strand at 150.558 kb on + strand at 150.588 kb on + strand at 150.689 kb on + strand at 150.697 kb on - strand, within PGA1_262p01400 at 150.706 kb on + strand, within PGA1_262p01400 at 150.729 kb on - strand, within PGA1_262p01400 at 150.734 kb on - strand, within PGA1_262p01400 at 150.778 kb on + strand, within PGA1_262p01400 at 150.813 kb on + strand, within PGA1_262p01400 at 150.823 kb on + strand, within PGA1_262p01400 at 150.830 kb on + strand, within PGA1_262p01400 at 151.036 kb on - strand, within PGA1_262p01400
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 100 mM remove 147,628 - PGA1_262p01370 0.14 +0.0 147,690 + PGA1_262p01370 0.19 -0.0 147,712 + PGA1_262p01370 0.21 +0.1 147,778 + PGA1_262p01370 0.27 +0.1 147,786 - PGA1_262p01370 0.28 -0.8 147,846 + PGA1_262p01370 0.33 -0.5 147,859 - PGA1_262p01370 0.34 -1.6 147,893 - PGA1_262p01370 0.37 -0.3 148,068 - PGA1_262p01370 0.52 +1.3 148,111 - PGA1_262p01370 0.56 +1.0 148,119 + PGA1_262p01370 0.57 +0.8 148,121 - PGA1_262p01370 0.57 -0.4 148,177 + PGA1_262p01370 0.62 +0.7 148,192 + PGA1_262p01370 0.63 -1.3 148,304 + PGA1_262p01370 0.73 -0.7 148,379 - PGA1_262p01370 0.79 +0.7 148,441 - PGA1_262p01370 0.85 +1.0 148,578 - -0.0 148,643 + +0.7 148,765 - PGA1_262p01380 0.10 +0.4 148,775 - PGA1_262p01380 0.11 -1.0 148,791 + PGA1_262p01380 0.12 -0.7 149,019 + PGA1_262p01380 0.28 -0.7 149,019 + PGA1_262p01380 0.28 +0.1 149,103 - PGA1_262p01380 0.34 -1.3 149,117 + PGA1_262p01380 0.35 -0.7 149,124 - PGA1_262p01380 0.35 -0.3 149,125 - PGA1_262p01380 0.35 +0.4 149,471 + PGA1_262p01380 0.59 -0.3 149,536 - PGA1_262p01380 0.63 +0.2 149,536 - PGA1_262p01380 0.63 -1.2 149,726 + PGA1_262p01380 0.76 -0.5 149,726 + PGA1_262p01380 0.76 -0.5 149,918 + PGA1_262p01380 0.90 +0.7 149,926 - -1.0 150,019 + -0.4 150,019 + -1.0 150,019 + +0.1 150,019 + -0.2 150,027 - +0.4 150,027 - +1.2 150,066 + -2.2 150,092 - -0.1 150,134 - PGA1_262p01390 0.26 -1.2 150,150 - PGA1_262p01390 0.33 +0.0 150,267 - PGA1_262p01390 0.82 -0.6 150,278 + PGA1_262p01390 0.86 -0.5 150,286 - PGA1_262p01390 0.90 +0.9 150,286 - PGA1_262p01390 0.90 -0.2 150,358 + +1.3 150,366 - -1.3 150,447 + +0.1 150,470 - +1.2 150,558 + +0.5 150,588 + -0.5 150,689 + +0.5 150,697 - PGA1_262p01400 0.10 +0.5 150,706 + PGA1_262p01400 0.10 +0.5 150,729 - PGA1_262p01400 0.11 +0.4 150,734 - PGA1_262p01400 0.12 +0.3 150,778 + PGA1_262p01400 0.13 +0.1 150,813 + PGA1_262p01400 0.15 +0.4 150,823 + PGA1_262p01400 0.15 -0.9 150,830 + PGA1_262p01400 0.15 +0.4 151,036 - PGA1_262p01400 0.24 -0.1
Or see this region's nucleotide sequence