Experiment: m.b. acetate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02030 and PGA1_c02040 overlap by 4 nucleotides PGA1_c02040 and PGA1_c02050 are separated by 199 nucleotides PGA1_c02050 and PGA1_c02060 are separated by 224 nucleotides
PGA1_c02030: PGA1_c02030 - phosphoglycerate mutase-like protein, at 185,912 to 186,409
_c02030
PGA1_c02040: PGA1_c02040 - hypothetical protein, at 186,406 to 187,149
_c02040
PGA1_c02050: PGA1_c02050 - acetylglutamate kinase ArgB, at 187,349 to 188,209
_c02050
PGA1_c02060: PGA1_c02060 - short-chain dehydrogenase / reductase, at 188,434 to 189,162
_c02060
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 186.353 kb on + strand, within PGA1_c02030 at 186.391 kb on + strand at 186.457 kb on + strand at 186.527 kb on + strand, within PGA1_c02040 at 186.535 kb on - strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.625 kb on + strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.656 kb on - strand, within PGA1_c02040 at 186.769 kb on - strand, within PGA1_c02040 at 186.779 kb on - strand, within PGA1_c02040 at 186.871 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.997 kb on - strand, within PGA1_c02040 at 186.999 kb on + strand, within PGA1_c02040 at 187.005 kb on + strand, within PGA1_c02040 at 187.007 kb on + strand, within PGA1_c02040 at 187.013 kb on - strand, within PGA1_c02040 at 187.144 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on - strand at 187.225 kb on + strand at 187.244 kb on - strand at 187.347 kb on + strand at 187.355 kb on - strand at 187.355 kb on - strand at 187.446 kb on + strand, within PGA1_c02050 at 187.454 kb on - strand, within PGA1_c02050 at 187.707 kb on + strand, within PGA1_c02050 at 187.707 kb on + strand, within PGA1_c02050 at 187.772 kb on + strand, within PGA1_c02050 at 187.897 kb on - strand, within PGA1_c02050 at 188.020 kb on + strand, within PGA1_c02050 at 188.028 kb on - strand, within PGA1_c02050 at 188.033 kb on - strand, within PGA1_c02050 at 188.064 kb on + strand, within PGA1_c02050 at 188.162 kb on + strand at 188.162 kb on + strand at 188.219 kb on + strand at 188.219 kb on + strand at 188.219 kb on + strand at 188.219 kb on + strand at 188.224 kb on + strand at 188.227 kb on - strand at 188.227 kb on - strand at 188.227 kb on - strand at 188.227 kb on - strand at 188.263 kb on + strand at 188.274 kb on + strand at 188.279 kb on + strand at 188.279 kb on + strand at 188.279 kb on + strand at 188.287 kb on - strand at 188.293 kb on + strand at 188.318 kb on - strand at 188.320 kb on - strand at 188.355 kb on - strand at 188.412 kb on + strand at 188.418 kb on + strand at 188.470 kb on - strand at 188.499 kb on + strand at 188.499 kb on + strand at 188.499 kb on - strand at 188.529 kb on - strand, within PGA1_c02060 at 188.541 kb on + strand, within PGA1_c02060 at 188.549 kb on - strand, within PGA1_c02060 at 188.565 kb on + strand, within PGA1_c02060 at 188.596 kb on + strand, within PGA1_c02060 at 188.673 kb on + strand, within PGA1_c02060 at 188.796 kb on - strand, within PGA1_c02060 at 188.869 kb on + strand, within PGA1_c02060 at 188.892 kb on - strand, within PGA1_c02060 at 188.898 kb on - strand, within PGA1_c02060 at 188.920 kb on + strand, within PGA1_c02060 at 188.999 kb on - strand, within PGA1_c02060 at 188.999 kb on - strand, within PGA1_c02060 at 189.048 kb on + strand, within PGA1_c02060 at 189.111 kb on - strand at 189.154 kb on - strand at 189.168 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 100 mM remove 186,353 + PGA1_c02030 0.89 -1.6 186,391 + -0.8 186,457 + -1.6 186,527 + PGA1_c02040 0.16 +1.5 186,535 - PGA1_c02040 0.17 +0.7 186,610 + PGA1_c02040 0.27 +0.7 186,610 + PGA1_c02040 0.27 -0.2 186,610 + PGA1_c02040 0.27 -0.2 186,618 - PGA1_c02040 0.28 +1.9 186,618 - PGA1_c02040 0.28 -0.7 186,618 - PGA1_c02040 0.28 +0.1 186,625 + PGA1_c02040 0.29 -0.8 186,651 - PGA1_c02040 0.33 +0.1 186,651 - PGA1_c02040 0.33 -0.3 186,656 - PGA1_c02040 0.34 +0.4 186,769 - PGA1_c02040 0.49 +1.2 186,779 - PGA1_c02040 0.50 -0.2 186,871 + PGA1_c02040 0.62 -0.0 186,903 + PGA1_c02040 0.67 -1.3 186,903 + PGA1_c02040 0.67 -0.9 186,997 - PGA1_c02040 0.79 +2.1 186,999 + PGA1_c02040 0.80 +0.6 187,005 + PGA1_c02040 0.81 +0.4 187,007 + PGA1_c02040 0.81 -0.3 187,013 - PGA1_c02040 0.82 +0.3 187,144 + -1.2 187,193 + -2.2 187,193 + -0.8 187,193 + +0.6 187,193 - +0.4 187,225 + +0.0 187,244 - +0.6 187,347 + +0.0 187,355 - -0.1 187,355 - +0.5 187,446 + PGA1_c02050 0.11 -0.9 187,454 - PGA1_c02050 0.12 -1.2 187,707 + PGA1_c02050 0.42 -1.1 187,707 + PGA1_c02050 0.42 -2.8 187,772 + PGA1_c02050 0.49 -1.6 187,897 - PGA1_c02050 0.64 -2.0 188,020 + PGA1_c02050 0.78 -2.4 188,028 - PGA1_c02050 0.79 -1.1 188,033 - PGA1_c02050 0.79 -1.8 188,064 + PGA1_c02050 0.83 +0.1 188,162 + +0.3 188,162 + -1.8 188,219 + +0.5 188,219 + +0.0 188,219 + +1.7 188,219 + +0.2 188,224 + +0.7 188,227 - -0.5 188,227 - -0.1 188,227 - -0.9 188,227 - +0.4 188,263 + -0.7 188,274 + -2.1 188,279 + +0.5 188,279 + +0.9 188,279 + -0.6 188,287 - +0.2 188,293 + -0.8 188,318 - +1.5 188,320 - +2.1 188,355 - +0.7 188,412 + +0.3 188,418 + -0.2 188,470 - -0.5 188,499 + -1.6 188,499 + -0.3 188,499 - +0.6 188,529 - PGA1_c02060 0.13 -0.2 188,541 + PGA1_c02060 0.15 -1.2 188,549 - PGA1_c02060 0.16 +0.5 188,565 + PGA1_c02060 0.18 +0.4 188,596 + PGA1_c02060 0.22 -0.3 188,673 + PGA1_c02060 0.33 +0.8 188,796 - PGA1_c02060 0.50 +0.2 188,869 + PGA1_c02060 0.60 -0.5 188,892 - PGA1_c02060 0.63 +0.7 188,898 - PGA1_c02060 0.64 +0.2 188,920 + PGA1_c02060 0.67 -0.6 188,999 - PGA1_c02060 0.78 -0.3 188,999 - PGA1_c02060 0.78 -0.2 189,048 + PGA1_c02060 0.84 -0.8 189,111 - +0.3 189,154 - -1.0 189,168 + -0.4
Or see this region's nucleotide sequence