Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_23245

Experiment: 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_23240 and AO356_23245 are separated by 152 nucleotidesAO356_23245 and AO356_23250 are separated by 138 nucleotidesAO356_23250 and AO356_23255 are separated by 201 nucleotides AO356_23240: AO356_23240 - hypothetical protein, at 5,201,660 to 5,202,109 _23240 AO356_23245: AO356_23245 - ABC transporter substrate-binding protein, at 5,202,262 to 5,203,272 _23245 AO356_23250: AO356_23250 - hypothetical protein, at 5,203,411 to 5,203,848 _23250 AO356_23255: AO356_23255 - cation:proton antiporter, at 5,204,050 to 5,204,412 _23255 Position (kb) 5202 5203 5204Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5201.271 kb on + strandat 5201.280 kb on + strandat 5201.305 kb on - strandat 5201.338 kb on - strandat 5201.341 kb on + strandat 5201.342 kb on - strandat 5201.342 kb on - strandat 5201.384 kb on + strandat 5201.407 kb on + strandat 5201.407 kb on + strandat 5201.407 kb on + strandat 5201.407 kb on + strandat 5201.408 kb on - strandat 5201.408 kb on - strandat 5201.411 kb on - strandat 5201.411 kb on - strandat 5201.496 kb on + strandat 5201.496 kb on + strandat 5201.497 kb on - strandat 5201.497 kb on - strandat 5201.568 kb on - strandat 5201.646 kb on + strandat 5201.646 kb on + strandat 5201.646 kb on + strandat 5201.646 kb on + strandat 5201.647 kb on - strandat 5201.647 kb on - strandat 5201.647 kb on - strandat 5201.647 kb on - strandat 5201.650 kb on + strandat 5201.650 kb on + strandat 5201.650 kb on + strandat 5201.651 kb on - strandat 5201.651 kb on - strandat 5201.651 kb on - strandat 5201.670 kb on + strandat 5201.670 kb on + strandat 5201.670 kb on + strandat 5201.670 kb on + strandat 5201.677 kb on - strandat 5201.729 kb on + strand, within AO356_23240at 5201.755 kb on + strand, within AO356_23240at 5201.756 kb on - strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.759 kb on + strand, within AO356_23240at 5201.760 kb on - strand, within AO356_23240at 5201.760 kb on - strand, within AO356_23240at 5201.760 kb on - strand, within AO356_23240at 5201.912 kb on + strand, within AO356_23240at 5201.913 kb on - strand, within AO356_23240at 5202.193 kb on - strandat 5202.233 kb on - strandat 5202.240 kb on + strandat 5202.441 kb on + strand, within AO356_23245at 5202.441 kb on + strand, within AO356_23245at 5202.442 kb on - strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.494 kb on + strand, within AO356_23245at 5202.495 kb on - strand, within AO356_23245at 5202.495 kb on - strand, within AO356_23245at 5202.495 kb on - strand, within AO356_23245at 5202.495 kb on - strand, within AO356_23245at 5202.620 kb on - strand, within AO356_23245at 5202.620 kb on - strand, within AO356_23245at 5202.620 kb on - strand, within AO356_23245at 5202.697 kb on + strand, within AO356_23245at 5202.802 kb on + strand, within AO356_23245at 5202.803 kb on - strand, within AO356_23245at 5202.820 kb on + strand, within AO356_23245at 5202.820 kb on + strand, within AO356_23245at 5202.820 kb on + strand, within AO356_23245at 5202.821 kb on - strand, within AO356_23245at 5202.904 kb on + strand, within AO356_23245at 5202.904 kb on + strand, within AO356_23245at 5202.904 kb on + strand, within AO356_23245at 5202.904 kb on + strand, within AO356_23245at 5202.905 kb on - strand, within AO356_23245at 5202.905 kb on - strand, within AO356_23245at 5202.905 kb on - strand, within AO356_23245at 5202.905 kb on - strand, within AO356_23245at 5202.907 kb on + strand, within AO356_23245at 5202.907 kb on + strand, within AO356_23245at 5202.908 kb on - strand, within AO356_23245at 5203.270 kb on + strandat 5203.270 kb on + strandat 5203.375 kb on - strandat 5203.375 kb on - strandat 5203.375 kb on - strandat 5203.422 kb on - strandat 5203.422 kb on - strandat 5203.474 kb on + strand, within AO356_23250at 5203.474 kb on + strand, within AO356_23250at 5203.475 kb on - strand, within AO356_23250at 5203.792 kb on + strand, within AO356_23250at 5203.875 kb on + strandat 5203.875 kb on + strandat 5203.876 kb on - strandat 5203.876 kb on - strandat 5203.876 kb on - strandat 5203.876 kb on - strandat 5204.045 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 20 mM putrescine
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5,201,271 + +0.5
5,201,280 + +0.3
5,201,305 - +0.5
5,201,338 - +0.1
5,201,341 + -1.5
5,201,342 - -0.7
5,201,342 - -0.3
5,201,384 + -0.8
5,201,407 + -0.8
5,201,407 + -1.0
5,201,407 + +0.3
5,201,407 + -0.7
5,201,408 - -1.5
5,201,408 - +0.3
5,201,411 - +1.6
5,201,411 - +0.6
5,201,496 + -3.3
5,201,496 + -1.5
5,201,497 - +2.1
5,201,497 - +0.7
5,201,568 - +0.9
5,201,646 + +1.1
5,201,646 + -0.2
5,201,646 + +0.8
5,201,646 + +0.1
5,201,647 - +0.8
5,201,647 - -0.5
5,201,647 - +0.6
5,201,647 - +0.5
5,201,650 + +0.1
5,201,650 + -0.7
5,201,650 + -0.1
5,201,651 - -0.2
5,201,651 - -0.6
5,201,651 - +0.1
5,201,670 + -0.3
5,201,670 + +1.1
5,201,670 + +0.9
5,201,670 + +1.1
5,201,677 - -0.5
5,201,729 + AO356_23240 0.15 +0.3
5,201,755 + AO356_23240 0.21 +0.3
5,201,756 - AO356_23240 0.21 +0.7
5,201,759 + AO356_23240 0.22 +0.6
5,201,759 + AO356_23240 0.22 +0.9
5,201,759 + AO356_23240 0.22 -0.1
5,201,759 + AO356_23240 0.22 +0.3
5,201,759 + AO356_23240 0.22 +0.6
5,201,759 + AO356_23240 0.22 +0.1
5,201,759 + AO356_23240 0.22 +0.3
5,201,759 + AO356_23240 0.22 -0.2
5,201,759 + AO356_23240 0.22 +0.1
5,201,759 + AO356_23240 0.22 +1.0
5,201,760 - AO356_23240 0.22 +1.2
5,201,760 - AO356_23240 0.22 -0.4
5,201,760 - AO356_23240 0.22 +1.3
5,201,912 + AO356_23240 0.56 +0.7
5,201,913 - AO356_23240 0.56 +0.2
5,202,193 - -0.1
5,202,233 - +0.2
5,202,240 + +0.8
5,202,441 + AO356_23245 0.18 +0.2
5,202,441 + AO356_23245 0.18 +1.9
5,202,442 - AO356_23245 0.18 -0.0
5,202,494 + AO356_23245 0.23 +0.5
5,202,494 + AO356_23245 0.23 +0.1
5,202,494 + AO356_23245 0.23 -0.3
5,202,494 + AO356_23245 0.23 +0.2
5,202,494 + AO356_23245 0.23 +1.0
5,202,494 + AO356_23245 0.23 +1.1
5,202,495 - AO356_23245 0.23 +0.0
5,202,495 - AO356_23245 0.23 +0.3
5,202,495 - AO356_23245 0.23 +0.4
5,202,495 - AO356_23245 0.23 +1.4
5,202,620 - AO356_23245 0.35 +0.1
5,202,620 - AO356_23245 0.35 +0.0
5,202,620 - AO356_23245 0.35 +1.1
5,202,697 + AO356_23245 0.43 +0.5
5,202,802 + AO356_23245 0.53 +0.7
5,202,803 - AO356_23245 0.54 +0.5
5,202,820 + AO356_23245 0.55 +0.1
5,202,820 + AO356_23245 0.55 -0.1
5,202,820 + AO356_23245 0.55 -0.5
5,202,821 - AO356_23245 0.55 -1.3
5,202,904 + AO356_23245 0.64 +1.2
5,202,904 + AO356_23245 0.64 +0.8
5,202,904 + AO356_23245 0.64 -0.1
5,202,904 + AO356_23245 0.64 +0.1
5,202,905 - AO356_23245 0.64 +0.0
5,202,905 - AO356_23245 0.64 -0.3
5,202,905 - AO356_23245 0.64 -0.1
5,202,905 - AO356_23245 0.64 -1.8
5,202,907 + AO356_23245 0.64 +0.1
5,202,907 + AO356_23245 0.64 -0.5
5,202,908 - AO356_23245 0.64 +1.3
5,203,270 + -0.1
5,203,270 + +1.0
5,203,375 - -0.8
5,203,375 - -0.1
5,203,375 - +0.3
5,203,422 - -0.5
5,203,422 - +0.1
5,203,474 + AO356_23250 0.14 -1.0
5,203,474 + AO356_23250 0.14 -0.9
5,203,475 - AO356_23250 0.15 -0.3
5,203,792 + AO356_23250 0.87 -1.3
5,203,875 + +0.3
5,203,875 + +2.0
5,203,876 - +1.2
5,203,876 - +1.5
5,203,876 - -0.5
5,203,876 - -0.7
5,204,045 - -0.1

Or see this region's nucleotide sequence