Experiment: BG11 with Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagA and purE are separated by 49 nucleotides purE and amt1 are separated by 266 nucleotides
Synpcc7942_0440: nagA - N-acetylglucosamine 6-phosphate deacetylase, at 429,346 to 430,473
nagA
Synpcc7942_0441: purE - phosphoribosylaminoimidazole carboxylase, catalytic subunit, at 430,523 to 431,191
purE
Synpcc7942_0442: amt1 - ammonium transporter, at 431,458 to 432,951
amt1
Position (kb) 430
431
432 Strain fitness (log2 ratio) -1
0
1
2 at 430.551 kb on - strand at 430.570 kb on + strand at 430.570 kb on + strand at 430.587 kb on + strand at 430.611 kb on + strand, within purE at 430.618 kb on - strand, within purE at 430.627 kb on - strand, within purE at 431.217 kb on + strand at 431.225 kb on + strand at 431.233 kb on - strand at 431.306 kb on + strand at 431.336 kb on - strand at 431.404 kb on + strand at 431.412 kb on + strand at 431.420 kb on - strand at 431.439 kb on + strand at 431.522 kb on + strand at 431.525 kb on - strand at 431.554 kb on - strand at 431.586 kb on + strand at 431.625 kb on + strand, within amt1 at 431.633 kb on - strand, within amt1 at 431.633 kb on - strand, within amt1 at 431.633 kb on - strand, within amt1 at 431.635 kb on + strand, within amt1 at 431.654 kb on + strand, within amt1 at 431.697 kb on + strand, within amt1 at 431.705 kb on - strand, within amt1 at 431.754 kb on + strand, within amt1 at 431.759 kb on + strand, within amt1 at 431.762 kb on - strand, within amt1 at 431.941 kb on - strand, within amt1 at 432.009 kb on + strand, within amt1 at 432.009 kb on + strand, within amt1 at 432.047 kb on - strand, within amt1 at 432.100 kb on + strand, within amt1 at 432.140 kb on + strand, within amt1
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.125 mg/ml remove 430,551 - -0.7 430,570 + -0.8 430,570 + -1.2 430,587 + +1.3 430,611 + purE Synpcc7942_0441 0.13 -0.0 430,618 - purE Synpcc7942_0441 0.14 +1.3 430,627 - purE Synpcc7942_0441 0.16 +0.7 431,217 + -0.5 431,225 + -0.7 431,233 - +0.5 431,306 + +0.5 431,336 - +0.3 431,404 + +1.5 431,412 + +1.5 431,420 - +0.9 431,439 + -0.3 431,522 + -0.1 431,525 - +1.8 431,554 - +0.7 431,586 + +1.0 431,625 + amt1 Synpcc7942_0442 0.11 +0.0 431,633 - amt1 Synpcc7942_0442 0.12 +0.9 431,633 - amt1 Synpcc7942_0442 0.12 +1.7 431,633 - amt1 Synpcc7942_0442 0.12 +0.0 431,635 + amt1 Synpcc7942_0442 0.12 +0.0 431,654 + amt1 Synpcc7942_0442 0.13 +1.4 431,697 + amt1 Synpcc7942_0442 0.16 +0.9 431,705 - amt1 Synpcc7942_0442 0.17 +0.4 431,754 + amt1 Synpcc7942_0442 0.20 +1.7 431,759 + amt1 Synpcc7942_0442 0.20 -0.0 431,762 - amt1 Synpcc7942_0442 0.20 +0.3 431,941 - amt1 Synpcc7942_0442 0.32 +1.2 432,009 + amt1 Synpcc7942_0442 0.37 +1.1 432,009 + amt1 Synpcc7942_0442 0.37 +0.1 432,047 - amt1 Synpcc7942_0442 0.39 +1.5 432,100 + amt1 Synpcc7942_0442 0.43 +0.5 432,140 + amt1 Synpcc7942_0442 0.46 +1.1
Or see this region's nucleotide sequence