Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23915 and BWI76_RS23920 are separated by 377 nucleotides BWI76_RS23920 and BWI76_RS23925 are separated by 269 nucleotides
BWI76_RS23915: BWI76_RS23915 - 5-formyltetrahydrofolate cyclo-ligase, at 4,990,550 to 4,991,146
_RS23915
BWI76_RS23920: BWI76_RS23920 - D-3-phosphoglycerate dehydrogenase, at 4,991,524 to 4,992,756
_RS23920
BWI76_RS23925: BWI76_RS23925 - ribose-5-phosphate isomerase, at 4,993,026 to 4,993,685
_RS23925
Position (kb)
4991
4992
4993 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4990.770 kb on - strand, within BWI76_RS23915 at 4991.082 kb on + strand, within BWI76_RS23915 at 4991.197 kb on + strand at 4991.198 kb on + strand at 4991.205 kb on - strand at 4991.354 kb on - strand at 4991.744 kb on + strand, within BWI76_RS23920 at 4991.783 kb on + strand, within BWI76_RS23920 at 4991.801 kb on - strand, within BWI76_RS23920 at 4991.924 kb on - strand, within BWI76_RS23920 at 4992.264 kb on - strand, within BWI76_RS23920 at 4992.658 kb on + strand at 4992.671 kb on + strand at 4992.746 kb on + strand at 4992.746 kb on + strand at 4992.802 kb on - strand at 4993.048 kb on + strand at 4993.057 kb on + strand at 4993.407 kb on + strand, within BWI76_RS23925 at 4993.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,990,770 - BWI76_RS23915 0.37 -0.7 4,991,082 + BWI76_RS23915 0.89 -0.9 4,991,197 + +0.3 4,991,198 + -0.2 4,991,205 - -0.0 4,991,354 - +0.3 4,991,744 + BWI76_RS23920 0.18 -2.7 4,991,783 + BWI76_RS23920 0.21 -5.4 4,991,801 - BWI76_RS23920 0.22 -4.9 4,991,924 - BWI76_RS23920 0.32 -2.4 4,992,264 - BWI76_RS23920 0.60 -1.7 4,992,658 + -3.1 4,992,671 + -4.9 4,992,746 + -3.4 4,992,746 + -2.9 4,992,802 - -0.1 4,993,048 + -1.2 4,993,057 + -2.9 4,993,407 + BWI76_RS23925 0.58 -1.6 4,993,628 + -2.0
Or see this region's nucleotide sequence