Experiment: MRS + carbenicillin Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13150 and BBR_RS13155 are separated by 113 nucleotides BBR_RS13155 and BBR_RS13160 are separated by 123 nucleotides BBR_RS13160 and BBR_RS13165 are separated by 56 nucleotides
BBR_RS13150: BBR_RS13150 - DNA helicase RecQ, at 725,884 to 727,803
_RS13150
BBR_RS13155: BBR_RS13155 - S-ribosylhomocysteine lyase, at 727,917 to 728,411
_RS13155
BBR_RS13160: BBR_RS13160 - serine O-acetyltransferase, at 728,535 to 729,077
_RS13160
BBR_RS13165: BBR_RS13165 - amino acid permease, at 729,134 to 730,591
_RS13165
Position (kb)
727
728
729 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 726.925 kb on + strand, within BBR_RS13150 at 726.925 kb on + strand, within BBR_RS13150 at 726.925 kb on - strand, within BBR_RS13150 at 726.933 kb on - strand, within BBR_RS13150 at 726.951 kb on + strand, within BBR_RS13150 at 726.954 kb on + strand, within BBR_RS13150 at 726.959 kb on + strand, within BBR_RS13150 at 727.104 kb on - strand, within BBR_RS13150 at 727.180 kb on - strand, within BBR_RS13150 at 727.269 kb on - strand, within BBR_RS13150 at 727.278 kb on + strand, within BBR_RS13150 at 727.283 kb on + strand, within BBR_RS13150 at 727.291 kb on - strand, within BBR_RS13150 at 727.291 kb on - strand, within BBR_RS13150 at 727.319 kb on - strand, within BBR_RS13150 at 727.319 kb on - strand, within BBR_RS13150 at 727.319 kb on - strand, within BBR_RS13150 at 727.508 kb on - strand, within BBR_RS13150 at 727.537 kb on - strand, within BBR_RS13150 at 727.551 kb on - strand, within BBR_RS13150 at 727.558 kb on - strand, within BBR_RS13150 at 727.600 kb on - strand, within BBR_RS13150 at 727.720 kb on + strand at 727.721 kb on + strand at 727.736 kb on + strand at 727.840 kb on + strand at 727.840 kb on + strand at 727.863 kb on - strand at 727.871 kb on + strand at 727.871 kb on + strand at 727.871 kb on + strand at 727.933 kb on - strand at 727.960 kb on + strand at 727.964 kb on + strand at 727.968 kb on - strand, within BBR_RS13155 at 727.976 kb on + strand, within BBR_RS13155 at 727.976 kb on + strand, within BBR_RS13155 at 728.096 kb on + strand, within BBR_RS13155 at 728.101 kb on + strand, within BBR_RS13155 at 728.101 kb on + strand, within BBR_RS13155 at 728.101 kb on + strand, within BBR_RS13155 at 728.111 kb on + strand, within BBR_RS13155 at 728.113 kb on + strand, within BBR_RS13155 at 728.195 kb on + strand, within BBR_RS13155 at 728.212 kb on + strand, within BBR_RS13155 at 728.212 kb on + strand, within BBR_RS13155 at 728.233 kb on - strand, within BBR_RS13155 at 728.269 kb on - strand, within BBR_RS13155 at 728.283 kb on - strand, within BBR_RS13155 at 728.295 kb on + strand, within BBR_RS13155 at 728.303 kb on + strand, within BBR_RS13155 at 728.308 kb on + strand, within BBR_RS13155 at 728.345 kb on + strand, within BBR_RS13155 at 728.353 kb on - strand, within BBR_RS13155 at 728.358 kb on - strand, within BBR_RS13155 at 728.405 kb on + strand at 728.413 kb on - strand at 728.423 kb on - strand at 728.423 kb on - strand at 728.434 kb on - strand at 728.441 kb on - strand at 728.443 kb on - strand at 728.468 kb on - strand at 728.470 kb on + strand at 728.503 kb on - strand at 728.529 kb on - strand at 728.533 kb on + strand at 728.569 kb on - strand at 728.618 kb on + strand, within BBR_RS13160 at 728.640 kb on - strand, within BBR_RS13160 at 728.640 kb on - strand, within BBR_RS13160 at 728.650 kb on - strand, within BBR_RS13160 at 728.673 kb on - strand, within BBR_RS13160 at 728.681 kb on - strand, within BBR_RS13160 at 728.681 kb on - strand, within BBR_RS13160 at 728.713 kb on + strand, within BBR_RS13160 at 728.721 kb on - strand, within BBR_RS13160 at 728.728 kb on - strand, within BBR_RS13160 at 728.730 kb on + strand, within BBR_RS13160 at 728.730 kb on + strand, within BBR_RS13160 at 728.736 kb on - strand, within BBR_RS13160 at 728.736 kb on - strand, within BBR_RS13160 at 728.738 kb on - strand, within BBR_RS13160 at 728.775 kb on - strand, within BBR_RS13160 at 728.782 kb on + strand, within BBR_RS13160 at 728.793 kb on + strand, within BBR_RS13160 at 728.816 kb on + strand, within BBR_RS13160 at 728.895 kb on + strand, within BBR_RS13160 at 728.942 kb on + strand, within BBR_RS13160 at 728.960 kb on + strand, within BBR_RS13160 at 728.968 kb on - strand, within BBR_RS13160 at 728.968 kb on - strand, within BBR_RS13160 at 728.968 kb on - strand, within BBR_RS13160 at 729.110 kb on - strand at 729.132 kb on + strand at 729.132 kb on + strand at 729.140 kb on - strand at 729.150 kb on + strand at 729.158 kb on - strand at 729.170 kb on - strand at 729.170 kb on - strand at 729.262 kb on - strand at 729.296 kb on + strand, within BBR_RS13165 at 729.301 kb on - strand, within BBR_RS13165 at 729.313 kb on - strand, within BBR_RS13165 at 729.328 kb on + strand, within BBR_RS13165 at 729.328 kb on + strand, within BBR_RS13165 at 729.336 kb on - strand, within BBR_RS13165 at 729.366 kb on + strand, within BBR_RS13165
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time24hr remove 726,925 + BBR_RS13150 0.54 -0.4 726,925 + BBR_RS13150 0.54 +3.2 726,925 - BBR_RS13150 0.54 +0.8 726,933 - BBR_RS13150 0.55 -0.9 726,951 + BBR_RS13150 0.56 +2.2 726,954 + BBR_RS13150 0.56 +0.8 726,959 + BBR_RS13150 0.56 +0.1 727,104 - BBR_RS13150 0.64 -2.0 727,180 - BBR_RS13150 0.68 -0.6 727,269 - BBR_RS13150 0.72 -0.3 727,278 + BBR_RS13150 0.73 -1.4 727,283 + BBR_RS13150 0.73 +0.7 727,291 - BBR_RS13150 0.73 -0.4 727,291 - BBR_RS13150 0.73 +0.3 727,319 - BBR_RS13150 0.75 -0.9 727,319 - BBR_RS13150 0.75 -1.0 727,319 - BBR_RS13150 0.75 -0.5 727,508 - BBR_RS13150 0.85 +0.1 727,537 - BBR_RS13150 0.86 +1.5 727,551 - BBR_RS13150 0.87 +0.5 727,558 - BBR_RS13150 0.87 -0.5 727,600 - BBR_RS13150 0.89 +3.0 727,720 + +0.8 727,721 + -1.3 727,736 + -1.1 727,840 + -1.2 727,840 + +0.1 727,863 - +0.5 727,871 + +1.5 727,871 + +0.4 727,871 + +0.3 727,933 - -1.5 727,960 + -1.2 727,964 + +2.7 727,968 - BBR_RS13155 0.10 +0.7 727,976 + BBR_RS13155 0.12 +0.5 727,976 + BBR_RS13155 0.12 -1.8 728,096 + BBR_RS13155 0.36 +0.5 728,101 + BBR_RS13155 0.37 -0.9 728,101 + BBR_RS13155 0.37 -0.7 728,101 + BBR_RS13155 0.37 -2.0 728,111 + BBR_RS13155 0.39 +0.5 728,113 + BBR_RS13155 0.40 -2.2 728,195 + BBR_RS13155 0.56 -1.5 728,212 + BBR_RS13155 0.60 -2.5 728,212 + BBR_RS13155 0.60 -1.3 728,233 - BBR_RS13155 0.64 -1.5 728,269 - BBR_RS13155 0.71 -1.5 728,283 - BBR_RS13155 0.74 +0.2 728,295 + BBR_RS13155 0.76 +0.1 728,303 + BBR_RS13155 0.78 -1.5 728,308 + BBR_RS13155 0.79 -0.9 728,345 + BBR_RS13155 0.86 +0.3 728,353 - BBR_RS13155 0.88 -3.4 728,358 - BBR_RS13155 0.89 -1.5 728,405 + +0.6 728,413 - -1.5 728,423 - -0.3 728,423 - +1.3 728,434 - +1.5 728,441 - -1.5 728,443 - -3.3 728,468 - +2.7 728,470 + +0.5 728,503 - +0.5 728,529 - +1.7 728,533 + -4.7 728,569 - +2.0 728,618 + BBR_RS13160 0.15 +0.1 728,640 - BBR_RS13160 0.19 -1.0 728,640 - BBR_RS13160 0.19 +0.3 728,650 - BBR_RS13160 0.21 -0.7 728,673 - BBR_RS13160 0.25 -1.5 728,681 - BBR_RS13160 0.27 +2.1 728,681 - BBR_RS13160 0.27 +0.4 728,713 + BBR_RS13160 0.33 +0.1 728,721 - BBR_RS13160 0.34 +2.0 728,728 - BBR_RS13160 0.36 +2.2 728,730 + BBR_RS13160 0.36 +2.6 728,730 + BBR_RS13160 0.36 -0.9 728,736 - BBR_RS13160 0.37 -0.1 728,736 - BBR_RS13160 0.37 +1.0 728,738 - BBR_RS13160 0.37 -1.5 728,775 - BBR_RS13160 0.44 +2.8 728,782 + BBR_RS13160 0.45 +1.4 728,793 + BBR_RS13160 0.48 -0.4 728,816 + BBR_RS13160 0.52 -2.9 728,895 + BBR_RS13160 0.66 -0.7 728,942 + BBR_RS13160 0.75 -2.0 728,960 + BBR_RS13160 0.78 -0.4 728,968 - BBR_RS13160 0.80 -1.5 728,968 - BBR_RS13160 0.80 +0.7 728,968 - BBR_RS13160 0.80 +2.7 729,110 - -1.2 729,132 + -0.5 729,132 + +2.5 729,140 - -0.6 729,150 + +0.8 729,158 - -0.5 729,170 - -1.5 729,170 - +0.1 729,262 - -2.6 729,296 + BBR_RS13165 0.11 -0.3 729,301 - BBR_RS13165 0.11 -0.2 729,313 - BBR_RS13165 0.12 +0.0 729,328 + BBR_RS13165 0.13 -0.6 729,328 + BBR_RS13165 0.13 -1.1 729,336 - BBR_RS13165 0.14 +2.3 729,366 + BBR_RS13165 0.16 -0.2
Or see this region's nucleotide sequence