Experiment: BG11 with Fusidic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and Synpcc7942_0712 are separated by 175 nucleotides Synpcc7942_0712 and ispG are separated by 99 nucleotides
Synpcc7942_0711: carB - carbamoyl phosphate synthase large subunit, at 701,604 to 704,897
carB
Synpcc7942_0712: Synpcc7942_0712 - C-terminal processing peptidase-2. Serine peptidase. MEROPS family S41A, at 705,073 to 706,350
_0712
Synpcc7942_0713: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 706,450 to 707,673
ispG
Position (kb) 705
706
707 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 704.938 kb on - strand at 705.135 kb on - strand at 705.186 kb on + strand at 705.193 kb on + strand at 705.254 kb on + strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.354 kb on - strand, within Synpcc7942_0712 at 705.447 kb on + strand, within Synpcc7942_0712 at 705.455 kb on - strand, within Synpcc7942_0712 at 705.462 kb on - strand, within Synpcc7942_0712 at 705.553 kb on + strand, within Synpcc7942_0712 at 705.618 kb on + strand, within Synpcc7942_0712 at 705.945 kb on + strand, within Synpcc7942_0712 at 705.969 kb on - strand, within Synpcc7942_0712 at 706.004 kb on - strand, within Synpcc7942_0712 at 706.078 kb on + strand, within Synpcc7942_0712 at 706.086 kb on - strand, within Synpcc7942_0712 at 706.086 kb on - strand, within Synpcc7942_0712 at 706.091 kb on + strand, within Synpcc7942_0712 at 706.099 kb on - strand, within Synpcc7942_0712 at 706.101 kb on - strand, within Synpcc7942_0712 at 706.151 kb on + strand, within Synpcc7942_0712 at 706.248 kb on + strand at 706.282 kb on + strand at 706.352 kb on + strand at 706.360 kb on - strand at 706.387 kb on + strand at 706.387 kb on + strand at 706.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.25 mg/ml remove 704,938 - -1.4 705,135 - -0.6 705,186 + -1.2 705,193 + -1.2 705,254 + Synpcc7942_0712 0.14 -5.9 705,354 - Synpcc7942_0712 0.22 +2.7 705,354 - Synpcc7942_0712 0.22 +0.0 705,354 - Synpcc7942_0712 0.22 -2.4 705,447 + Synpcc7942_0712 0.29 -1.1 705,455 - Synpcc7942_0712 0.30 -3.7 705,462 - Synpcc7942_0712 0.30 +1.0 705,553 + Synpcc7942_0712 0.38 -3.3 705,618 + Synpcc7942_0712 0.43 +1.4 705,945 + Synpcc7942_0712 0.68 -2.0 705,969 - Synpcc7942_0712 0.70 -1.9 706,004 - Synpcc7942_0712 0.73 -0.8 706,078 + Synpcc7942_0712 0.79 -2.3 706,086 - Synpcc7942_0712 0.79 -1.3 706,086 - Synpcc7942_0712 0.79 -3.7 706,091 + Synpcc7942_0712 0.80 -0.6 706,099 - Synpcc7942_0712 0.80 -3.1 706,101 - Synpcc7942_0712 0.80 -1.0 706,151 + Synpcc7942_0712 0.84 -3.1 706,248 + -1.1 706,282 + -0.4 706,352 + -0.4 706,360 - -1.8 706,387 + -0.3 706,387 + -0.8 706,387 + -0.8
Or see this region's nucleotide sequence