Experiment: BG11 with Fusidic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nha4 and Synpcc7942_0547 are separated by 33 nucleotides Synpcc7942_0547 and Synpcc7942_0548 are separated by 56 nucleotides Synpcc7942_0548 and Synpcc7942_0549 are separated by 100 nucleotides Synpcc7942_0549 and Synpcc7942_0550 are separated by 8 nucleotides
Synpcc7942_0546: nha4 - Na+/H+ antiporter, at 527,876 to 530,023
nha4
Synpcc7942_0547: Synpcc7942_0547 - hypothetical protein, at 530,057 to 530,566
_0547
Synpcc7942_0548: Synpcc7942_0548 - hypothetical protein, at 530,623 to 531,783
_0548
Synpcc7942_0549: Synpcc7942_0549 - hypothetical protein, at 531,884 to 532,645
_0549
Synpcc7942_0550: Synpcc7942_0550 - hypothetical protein, at 532,654 to 533,037
_0550
Position (kb) 530
531
532 Strain fitness (log2 ratio) -2
-1
0
1
2 at 529.665 kb on + strand, within nha4 at 529.685 kb on - strand, within nha4 at 529.729 kb on - strand, within nha4 at 529.739 kb on - strand, within nha4 at 529.739 kb on - strand, within nha4 at 529.764 kb on + strand, within nha4 at 529.772 kb on - strand, within nha4 at 529.787 kb on - strand, within nha4 at 529.797 kb on + strand, within nha4 at 529.797 kb on + strand, within nha4 at 529.814 kb on + strand at 529.814 kb on + strand at 529.841 kb on + strand at 529.861 kb on + strand at 529.866 kb on + strand at 529.866 kb on + strand at 529.872 kb on - strand at 529.872 kb on - strand at 529.876 kb on + strand at 529.925 kb on + strand at 529.941 kb on - strand at 529.948 kb on + strand at 529.966 kb on - strand at 530.178 kb on + strand, within Synpcc7942_0547 at 530.246 kb on + strand, within Synpcc7942_0547 at 530.255 kb on + strand, within Synpcc7942_0547 at 530.261 kb on + strand, within Synpcc7942_0547 at 530.299 kb on - strand, within Synpcc7942_0547 at 530.332 kb on + strand, within Synpcc7942_0547 at 530.391 kb on + strand, within Synpcc7942_0547 at 530.415 kb on - strand, within Synpcc7942_0547 at 530.550 kb on + strand at 530.558 kb on - strand at 530.588 kb on - strand at 530.588 kb on - strand at 530.588 kb on - strand at 530.605 kb on - strand at 530.613 kb on - strand at 530.621 kb on + strand at 530.629 kb on - strand at 530.629 kb on - strand at 530.802 kb on - strand, within Synpcc7942_0548 at 530.952 kb on + strand, within Synpcc7942_0548 at 531.069 kb on - strand, within Synpcc7942_0548 at 531.138 kb on + strand, within Synpcc7942_0548 at 531.215 kb on + strand, within Synpcc7942_0548 at 531.223 kb on - strand, within Synpcc7942_0548 at 531.223 kb on - strand, within Synpcc7942_0548 at 531.223 kb on - strand, within Synpcc7942_0548 at 531.263 kb on + strand, within Synpcc7942_0548 at 531.284 kb on + strand, within Synpcc7942_0548 at 531.292 kb on - strand, within Synpcc7942_0548 at 531.329 kb on - strand, within Synpcc7942_0548 at 531.367 kb on - strand, within Synpcc7942_0548 at 531.407 kb on + strand, within Synpcc7942_0548 at 531.442 kb on + strand, within Synpcc7942_0548 at 531.454 kb on + strand, within Synpcc7942_0548 at 531.456 kb on + strand, within Synpcc7942_0548 at 531.462 kb on - strand, within Synpcc7942_0548 at 531.464 kb on - strand, within Synpcc7942_0548 at 531.466 kb on + strand, within Synpcc7942_0548 at 531.469 kb on - strand, within Synpcc7942_0548 at 531.475 kb on - strand, within Synpcc7942_0548 at 531.563 kb on + strand, within Synpcc7942_0548 at 531.563 kb on + strand, within Synpcc7942_0548 at 531.595 kb on + strand, within Synpcc7942_0548 at 531.607 kb on + strand, within Synpcc7942_0548 at 531.628 kb on + strand, within Synpcc7942_0548 at 531.683 kb on + strand at 531.683 kb on + strand at 531.822 kb on + strand at 531.828 kb on - strand at 531.839 kb on - strand at 531.851 kb on - strand at 532.611 kb on + strand at 532.611 kb on + strand at 532.655 kb on + strand at 532.737 kb on - strand, within Synpcc7942_0550 at 532.742 kb on + strand, within Synpcc7942_0550 at 532.742 kb on - strand, within Synpcc7942_0550 at 532.772 kb on - strand, within Synpcc7942_0550
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.25 mg/ml remove 529,665 + nha4 Synpcc7942_0546 0.83 +0.0 529,685 - nha4 Synpcc7942_0546 0.84 +0.0 529,729 - nha4 Synpcc7942_0546 0.86 +1.1 529,739 - nha4 Synpcc7942_0546 0.87 +0.0 529,739 - nha4 Synpcc7942_0546 0.87 -0.2 529,764 + nha4 Synpcc7942_0546 0.88 +1.2 529,772 - nha4 Synpcc7942_0546 0.88 -0.1 529,787 - nha4 Synpcc7942_0546 0.89 -0.0 529,797 + nha4 Synpcc7942_0546 0.89 -0.4 529,797 + nha4 Synpcc7942_0546 0.89 -0.7 529,814 + +0.5 529,814 + +0.2 529,841 + -1.4 529,861 + +1.1 529,866 + +1.0 529,866 + -1.5 529,872 - -0.1 529,872 - +0.6 529,876 + +1.0 529,925 + -0.0 529,941 - +0.5 529,948 + -0.3 529,966 - -0.2 530,178 + Synpcc7942_0547 0.24 +2.3 530,246 + Synpcc7942_0547 0.37 -0.3 530,255 + Synpcc7942_0547 0.39 +0.2 530,261 + Synpcc7942_0547 0.40 +0.2 530,299 - Synpcc7942_0547 0.47 +1.1 530,332 + Synpcc7942_0547 0.54 -0.3 530,391 + Synpcc7942_0547 0.65 -0.0 530,415 - Synpcc7942_0547 0.70 -0.5 530,550 + -2.3 530,558 - +0.2 530,588 - +0.8 530,588 - +0.6 530,588 - +0.1 530,605 - -0.6 530,613 - +0.6 530,621 + +0.7 530,629 - -1.4 530,629 - -0.5 530,802 - Synpcc7942_0548 0.15 -1.0 530,952 + Synpcc7942_0548 0.28 +0.5 531,069 - Synpcc7942_0548 0.38 -1.5 531,138 + Synpcc7942_0548 0.44 -2.7 531,215 + Synpcc7942_0548 0.51 -0.3 531,223 - Synpcc7942_0548 0.52 -0.3 531,223 - Synpcc7942_0548 0.52 -1.1 531,223 - Synpcc7942_0548 0.52 -1.9 531,263 + Synpcc7942_0548 0.55 -0.2 531,284 + Synpcc7942_0548 0.57 -0.3 531,292 - Synpcc7942_0548 0.58 -1.7 531,329 - Synpcc7942_0548 0.61 -2.6 531,367 - Synpcc7942_0548 0.64 +1.0 531,407 + Synpcc7942_0548 0.68 -0.7 531,442 + Synpcc7942_0548 0.71 -0.8 531,454 + Synpcc7942_0548 0.72 -1.3 531,456 + Synpcc7942_0548 0.72 -1.7 531,462 - Synpcc7942_0548 0.72 +0.2 531,464 - Synpcc7942_0548 0.72 -0.8 531,466 + Synpcc7942_0548 0.73 -0.6 531,469 - Synpcc7942_0548 0.73 -2.3 531,475 - Synpcc7942_0548 0.73 +0.0 531,563 + Synpcc7942_0548 0.81 -0.3 531,563 + Synpcc7942_0548 0.81 -0.5 531,595 + Synpcc7942_0548 0.84 -0.8 531,607 + Synpcc7942_0548 0.85 -0.8 531,628 + Synpcc7942_0548 0.87 -0.1 531,683 + -1.3 531,683 + -0.3 531,822 + -0.3 531,828 - -0.8 531,839 - -1.3 531,851 - +0.5 532,611 + +0.0 532,611 + -0.3 532,655 + +0.9 532,737 - Synpcc7942_0550 0.22 +0.6 532,742 + Synpcc7942_0550 0.23 -0.2 532,742 - Synpcc7942_0550 0.23 +0.5 532,772 - Synpcc7942_0550 0.31 -0.3
Or see this region's nucleotide sequence