Experiment: BG11 with Fusidic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0417 and Synpcc7942_0418 are separated by 134 nucleotides Synpcc7942_0418 and bioB are separated by 96 nucleotides
Synpcc7942_0417: Synpcc7942_0417 - ATPase, at 406,851 to 408,359
_0417
Synpcc7942_0418: Synpcc7942_0418 - hypothetical protein, at 408,494 to 408,844
_0418
Synpcc7942_0419: bioB - biotin synthase, at 408,941 to 409,903
bioB
Position (kb) 408
409 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 407.556 kb on + strand at 407.612 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.624 kb on + strand, within Synpcc7942_0417 at 407.657 kb on - strand, within Synpcc7942_0417 at 407.732 kb on + strand at 407.733 kb on + strand, within Synpcc7942_0417 at 407.740 kb on - strand, within Synpcc7942_0417 at 407.740 kb on - strand, within Synpcc7942_0417 at 407.756 kb on - strand, within Synpcc7942_0417 at 407.756 kb on - strand at 407.777 kb on - strand, within Synpcc7942_0417 at 407.850 kb on + strand, within Synpcc7942_0417 at 408.004 kb on + strand, within Synpcc7942_0417 at 408.101 kb on + strand, within Synpcc7942_0417 at 408.108 kb on - strand, within Synpcc7942_0417 at 408.179 kb on + strand, within Synpcc7942_0417 at 408.190 kb on + strand, within Synpcc7942_0417 at 408.213 kb on - strand at 408.223 kb on - strand at 408.262 kb on - strand at 408.303 kb on - strand at 408.318 kb on + strand at 408.388 kb on - strand at 408.394 kb on + strand at 408.404 kb on + strand at 408.456 kb on - strand at 408.497 kb on + strand at 408.504 kb on + strand at 408.504 kb on + strand at 408.504 kb on + strand at 408.541 kb on + strand, within Synpcc7942_0418 at 408.567 kb on + strand, within Synpcc7942_0418 at 408.601 kb on - strand, within Synpcc7942_0418 at 408.656 kb on + strand, within Synpcc7942_0418 at 408.687 kb on + strand, within Synpcc7942_0418 at 408.706 kb on + strand at 408.714 kb on - strand, within Synpcc7942_0418 at 408.714 kb on - strand, within Synpcc7942_0418 at 408.714 kb on - strand, within Synpcc7942_0418 at 408.806 kb on + strand, within Synpcc7942_0418 at 408.806 kb on + strand, within Synpcc7942_0418 at 408.814 kb on - strand at 408.814 kb on - strand at 408.844 kb on + strand at 408.844 kb on + strand at 408.881 kb on - strand at 408.881 kb on - strand at 408.881 kb on - strand at 408.908 kb on - strand at 408.938 kb on + strand at 408.942 kb on - strand at 408.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.25 mg/ml remove 407,556 + +1.1 407,612 + Synpcc7942_0417 0.50 -2.6 407,624 + Synpcc7942_0417 0.51 -1.6 407,624 + Synpcc7942_0417 0.51 -2.1 407,624 + Synpcc7942_0417 0.51 -2.0 407,657 - Synpcc7942_0417 0.53 +0.5 407,732 + +2.1 407,733 + Synpcc7942_0417 0.58 -0.9 407,740 - Synpcc7942_0417 0.59 -3.0 407,740 - Synpcc7942_0417 0.59 -1.9 407,756 - Synpcc7942_0417 0.60 -2.2 407,756 - +2.1 407,777 - Synpcc7942_0417 0.61 -0.2 407,850 + Synpcc7942_0417 0.66 -0.4 408,004 + Synpcc7942_0417 0.76 -0.2 408,101 + Synpcc7942_0417 0.83 +1.1 408,108 - Synpcc7942_0417 0.83 -0.9 408,179 + Synpcc7942_0417 0.88 -0.3 408,190 + Synpcc7942_0417 0.89 -0.6 408,213 - -1.0 408,223 - -0.1 408,262 - +0.7 408,303 - +0.5 408,318 + +0.8 408,388 - -0.8 408,394 + -2.1 408,404 + +2.1 408,456 - -1.6 408,497 + +0.5 408,504 + -1.0 408,504 + -2.6 408,504 + -4.1 408,541 + Synpcc7942_0418 0.13 -1.4 408,567 + Synpcc7942_0418 0.21 -1.3 408,601 - Synpcc7942_0418 0.30 -2.5 408,656 + Synpcc7942_0418 0.46 -1.7 408,687 + Synpcc7942_0418 0.55 -1.8 408,706 + +1.1 408,714 - Synpcc7942_0418 0.63 -0.4 408,714 - Synpcc7942_0418 0.63 -0.6 408,714 - Synpcc7942_0418 0.63 -1.2 408,806 + Synpcc7942_0418 0.89 +0.1 408,806 + Synpcc7942_0418 0.89 -1.4 408,814 - -2.1 408,814 - -1.0 408,844 + +0.7 408,844 + -1.1 408,881 - -1.8 408,881 - -0.9 408,881 - -0.3 408,908 - -0.2 408,938 + -0.2 408,942 - +0.2 408,953 - -0.9
Or see this region's nucleotide sequence