Experiment: m.b. syringaldehyde 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10620 and PGA1_c10630 are separated by 87 nucleotides PGA1_c10630 and PGA1_c10640 are separated by 52 nucleotides PGA1_c10640 and PGA1_c10650 overlap by 2 nucleotides PGA1_c10650 and PGA1_c10660 are separated by 12 nucleotides
PGA1_c10620: PGA1_c10620 - peptide chain release factor 3, at 1,099,571 to 1,101,178
_c10620
PGA1_c10630: PGA1_c10630 - SnoaL-like polyketide cyclase., at 1,101,266 to 1,101,835
_c10630
PGA1_c10640: PGA1_c10640 - hypothetical protein, at 1,101,888 to 1,103,201
_c10640
PGA1_c10650: PGA1_c10650 - putative short chain dehydrogenase, at 1,103,200 to 1,103,979
_c10650
PGA1_c10660: PGA1_c10660 - DNA alkylation repair enzyme, at 1,103,992 to 1,105,122
_c10660
Position (kb)
1101
1102
1103
1104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1101.222 kb on + strand at 1101.252 kb on - strand at 1101.263 kb on - strand at 1101.283 kb on - strand at 1101.342 kb on - strand, within PGA1_c10630 at 1101.386 kb on - strand, within PGA1_c10630 at 1101.426 kb on + strand, within PGA1_c10630 at 1101.426 kb on + strand, within PGA1_c10630 at 1101.431 kb on + strand, within PGA1_c10630 at 1101.434 kb on - strand, within PGA1_c10630 at 1101.445 kb on + strand, within PGA1_c10630 at 1101.450 kb on + strand, within PGA1_c10630 at 1101.512 kb on + strand, within PGA1_c10630 at 1101.550 kb on + strand, within PGA1_c10630 at 1101.550 kb on + strand, within PGA1_c10630 at 1101.550 kb on + strand, within PGA1_c10630 at 1101.558 kb on - strand, within PGA1_c10630 at 1101.558 kb on - strand, within PGA1_c10630 at 1101.563 kb on - strand, within PGA1_c10630 at 1101.570 kb on + strand, within PGA1_c10630 at 1101.684 kb on - strand, within PGA1_c10630 at 1101.738 kb on + strand, within PGA1_c10630 at 1101.819 kb on + strand at 1101.847 kb on + strand at 1101.865 kb on - strand at 1101.913 kb on + strand at 1101.913 kb on + strand at 1101.919 kb on + strand at 1101.936 kb on - strand at 1101.964 kb on - strand at 1102.039 kb on + strand, within PGA1_c10640 at 1102.039 kb on + strand, within PGA1_c10640 at 1102.039 kb on + strand, within PGA1_c10640 at 1102.047 kb on - strand, within PGA1_c10640 at 1102.047 kb on - strand, within PGA1_c10640 at 1102.053 kb on + strand, within PGA1_c10640 at 1102.061 kb on - strand, within PGA1_c10640 at 1102.061 kb on - strand, within PGA1_c10640 at 1102.062 kb on + strand, within PGA1_c10640 at 1102.246 kb on - strand, within PGA1_c10640 at 1102.251 kb on - strand, within PGA1_c10640 at 1102.251 kb on - strand, within PGA1_c10640 at 1102.417 kb on + strand, within PGA1_c10640 at 1102.422 kb on + strand, within PGA1_c10640 at 1102.422 kb on - strand, within PGA1_c10640 at 1102.435 kb on - strand, within PGA1_c10640 at 1102.454 kb on - strand, within PGA1_c10640 at 1102.498 kb on + strand, within PGA1_c10640 at 1102.511 kb on + strand, within PGA1_c10640 at 1102.519 kb on - strand, within PGA1_c10640 at 1102.537 kb on + strand, within PGA1_c10640 at 1102.594 kb on - strand, within PGA1_c10640 at 1102.664 kb on + strand, within PGA1_c10640 at 1102.687 kb on + strand, within PGA1_c10640 at 1102.694 kb on - strand, within PGA1_c10640 at 1102.742 kb on + strand, within PGA1_c10640 at 1102.796 kb on + strand, within PGA1_c10640 at 1102.804 kb on - strand, within PGA1_c10640 at 1102.804 kb on - strand, within PGA1_c10640 at 1102.871 kb on - strand, within PGA1_c10640 at 1102.871 kb on - strand, within PGA1_c10640 at 1102.881 kb on + strand, within PGA1_c10640 at 1102.889 kb on - strand, within PGA1_c10640 at 1102.912 kb on - strand, within PGA1_c10640 at 1102.975 kb on + strand, within PGA1_c10640 at 1103.087 kb on + strand at 1103.105 kb on + strand at 1103.106 kb on - strand at 1103.134 kb on + strand at 1103.134 kb on + strand at 1103.134 kb on + strand at 1103.144 kb on + strand at 1103.251 kb on + strand at 1103.257 kb on + strand at 1103.257 kb on + strand at 1103.257 kb on + strand at 1103.265 kb on - strand at 1103.265 kb on - strand at 1103.265 kb on - strand at 1103.270 kb on - strand at 1103.283 kb on - strand, within PGA1_c10650 at 1103.326 kb on - strand, within PGA1_c10650 at 1103.329 kb on + strand, within PGA1_c10650 at 1103.414 kb on + strand, within PGA1_c10650 at 1103.415 kb on + strand, within PGA1_c10650 at 1103.484 kb on + strand, within PGA1_c10650 at 1103.490 kb on + strand, within PGA1_c10650 at 1103.564 kb on - strand, within PGA1_c10650 at 1103.571 kb on - strand, within PGA1_c10650 at 1103.576 kb on + strand, within PGA1_c10650 at 1103.644 kb on + strand, within PGA1_c10650 at 1103.699 kb on + strand, within PGA1_c10650 at 1103.818 kb on + strand, within PGA1_c10650 at 1103.821 kb on - strand, within PGA1_c10650 at 1103.825 kb on + strand, within PGA1_c10650 at 1103.825 kb on + strand, within PGA1_c10650 at 1103.862 kb on + strand, within PGA1_c10650 at 1103.909 kb on - strand at 1103.932 kb on + strand at 1103.940 kb on - strand at 1104.198 kb on - strand, within PGA1_c10660 at 1104.198 kb on - strand, within PGA1_c10660 at 1104.198 kb on - strand, within PGA1_c10660
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. syringaldehyde 2 mM remove 1,101,222 + -0.7 1,101,252 - +1.4 1,101,263 - +0.8 1,101,283 - +0.8 1,101,342 - PGA1_c10630 0.13 -0.7 1,101,386 - PGA1_c10630 0.21 -0.9 1,101,426 + PGA1_c10630 0.28 -0.2 1,101,426 + PGA1_c10630 0.28 -0.0 1,101,431 + PGA1_c10630 0.29 +1.2 1,101,434 - PGA1_c10630 0.29 +0.3 1,101,445 + PGA1_c10630 0.31 -2.6 1,101,450 + PGA1_c10630 0.32 +0.1 1,101,512 + PGA1_c10630 0.43 -0.3 1,101,550 + PGA1_c10630 0.50 +0.4 1,101,550 + PGA1_c10630 0.50 +0.9 1,101,550 + PGA1_c10630 0.50 -0.1 1,101,558 - PGA1_c10630 0.51 +0.4 1,101,558 - PGA1_c10630 0.51 +0.6 1,101,563 - PGA1_c10630 0.52 +1.6 1,101,570 + PGA1_c10630 0.53 +2.2 1,101,684 - PGA1_c10630 0.73 -0.2 1,101,738 + PGA1_c10630 0.83 +0.2 1,101,819 + -0.1 1,101,847 + -0.0 1,101,865 - -0.2 1,101,913 + -0.1 1,101,913 + -1.5 1,101,919 + +0.1 1,101,936 - -0.3 1,101,964 - +1.3 1,102,039 + PGA1_c10640 0.11 +0.5 1,102,039 + PGA1_c10640 0.11 -0.6 1,102,039 + PGA1_c10640 0.11 +1.0 1,102,047 - PGA1_c10640 0.12 +0.9 1,102,047 - PGA1_c10640 0.12 +0.6 1,102,053 + PGA1_c10640 0.13 +0.5 1,102,061 - PGA1_c10640 0.13 -0.8 1,102,061 - PGA1_c10640 0.13 +0.5 1,102,062 + PGA1_c10640 0.13 -0.7 1,102,246 - PGA1_c10640 0.27 +1.1 1,102,251 - PGA1_c10640 0.28 +0.9 1,102,251 - PGA1_c10640 0.28 +0.2 1,102,417 + PGA1_c10640 0.40 -1.0 1,102,422 + PGA1_c10640 0.41 -0.6 1,102,422 - PGA1_c10640 0.41 -2.0 1,102,435 - PGA1_c10640 0.42 +0.4 1,102,454 - PGA1_c10640 0.43 -1.4 1,102,498 + PGA1_c10640 0.46 +0.1 1,102,511 + PGA1_c10640 0.47 +0.9 1,102,519 - PGA1_c10640 0.48 -0.8 1,102,537 + PGA1_c10640 0.49 +1.1 1,102,594 - PGA1_c10640 0.54 -1.2 1,102,664 + PGA1_c10640 0.59 +0.1 1,102,687 + PGA1_c10640 0.61 -0.1 1,102,694 - PGA1_c10640 0.61 +1.0 1,102,742 + PGA1_c10640 0.65 +1.8 1,102,796 + PGA1_c10640 0.69 -0.1 1,102,804 - PGA1_c10640 0.70 +1.4 1,102,804 - PGA1_c10640 0.70 +1.7 1,102,871 - PGA1_c10640 0.75 +0.5 1,102,871 - PGA1_c10640 0.75 -0.2 1,102,881 + PGA1_c10640 0.76 -0.2 1,102,889 - PGA1_c10640 0.76 -0.8 1,102,912 - PGA1_c10640 0.78 +1.6 1,102,975 + PGA1_c10640 0.83 -1.2 1,103,087 + +0.4 1,103,105 + +0.0 1,103,106 - +0.1 1,103,134 + -0.0 1,103,134 + +0.1 1,103,134 + -0.4 1,103,144 + -0.1 1,103,251 + +0.4 1,103,257 + +1.5 1,103,257 + +0.3 1,103,257 + -1.7 1,103,265 - +0.3 1,103,265 - -1.3 1,103,265 - +0.4 1,103,270 - -1.1 1,103,283 - PGA1_c10650 0.11 -0.2 1,103,326 - PGA1_c10650 0.16 -0.3 1,103,329 + PGA1_c10650 0.17 +0.6 1,103,414 + PGA1_c10650 0.27 +0.4 1,103,415 + PGA1_c10650 0.28 +0.7 1,103,484 + PGA1_c10650 0.36 -1.5 1,103,490 + PGA1_c10650 0.37 -0.6 1,103,564 - PGA1_c10650 0.47 -0.6 1,103,571 - PGA1_c10650 0.48 +0.2 1,103,576 + PGA1_c10650 0.48 -1.7 1,103,644 + PGA1_c10650 0.57 +0.4 1,103,699 + PGA1_c10650 0.64 -1.5 1,103,818 + PGA1_c10650 0.79 +0.2 1,103,821 - PGA1_c10650 0.80 +0.4 1,103,825 + PGA1_c10650 0.80 +0.9 1,103,825 + PGA1_c10650 0.80 +0.5 1,103,862 + PGA1_c10650 0.85 +0.7 1,103,909 - -0.3 1,103,932 + +1.2 1,103,940 - +0.9 1,104,198 - PGA1_c10660 0.18 +0.8 1,104,198 - PGA1_c10660 0.18 -0.1 1,104,198 - PGA1_c10660 0.18 +1.2
Or see this region's nucleotide sequence