Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25475 and BWI76_RS25480 are separated by 78 nucleotides BWI76_RS25480 and BWI76_RS25485 are separated by 2 nucleotides BWI76_RS25485 and BWI76_RS25490 are separated by 7 nucleotides
BWI76_RS25475: BWI76_RS25475 - TIGR02099 family protein, at 5,293,022 to 5,296,819
_RS25475
BWI76_RS25480: BWI76_RS25480 - ribonuclease E/G, at 5,296,898 to 5,298,367
_RS25480
BWI76_RS25485: BWI76_RS25485 - septum formation protein Maf, at 5,298,370 to 5,298,951
_RS25485
BWI76_RS25490: BWI76_RS25490 - rod shape-determining protein MreD, at 5,298,959 to 5,299,447
_RS25490
Position (kb)
5296
5297
5298
5299 Strain fitness (log2 ratio)
-1
0
1 at 5296.213 kb on + strand, within BWI76_RS25475 at 5296.223 kb on + strand, within BWI76_RS25475 at 5296.386 kb on - strand, within BWI76_RS25475 at 5296.386 kb on - strand, within BWI76_RS25475 at 5296.425 kb on + strand, within BWI76_RS25475 at 5296.860 kb on - strand at 5297.232 kb on - strand, within BWI76_RS25480 at 5297.312 kb on + strand, within BWI76_RS25480 at 5297.525 kb on - strand, within BWI76_RS25480 at 5297.591 kb on - strand, within BWI76_RS25480 at 5297.593 kb on + strand, within BWI76_RS25480 at 5297.654 kb on + strand, within BWI76_RS25480 at 5297.865 kb on + strand, within BWI76_RS25480 at 5298.411 kb on + strand at 5298.870 kb on - strand, within BWI76_RS25485
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 5,296,213 + BWI76_RS25475 0.84 -0.1 5,296,223 + BWI76_RS25475 0.84 -0.9 5,296,386 - BWI76_RS25475 0.89 -0.4 5,296,386 - BWI76_RS25475 0.89 +0.4 5,296,425 + BWI76_RS25475 0.90 -0.4 5,296,860 - +0.2 5,297,232 - BWI76_RS25480 0.23 -0.5 5,297,312 + BWI76_RS25480 0.28 +0.4 5,297,525 - BWI76_RS25480 0.43 -1.3 5,297,591 - BWI76_RS25480 0.47 -1.1 5,297,593 + BWI76_RS25480 0.47 +0.6 5,297,654 + BWI76_RS25480 0.51 -1.5 5,297,865 + BWI76_RS25480 0.66 -0.6 5,298,411 + -0.6 5,298,870 - BWI76_RS25485 0.86 +0.5
Or see this region's nucleotide sequence