Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23870

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23860 and BWI76_RS23865 are separated by 186 nucleotidesBWI76_RS23865 and BWI76_RS23870 are separated by 60 nucleotidesBWI76_RS23870 and BWI76_RS23875 are separated by 144 nucleotidesBWI76_RS23875 and BWI76_RS23880 are separated by 22 nucleotides BWI76_RS23860: BWI76_RS23860 - protein disulfide oxidoreductase, at 4,978,989 to 4,979,480 _RS23860 BWI76_RS23865: BWI76_RS23865 - NAD(P)-dependent oxidoreductase, at 4,979,667 to 4,980,410 _RS23865 BWI76_RS23870: BWI76_RS23870 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data), at 4,980,471 to 4,983,344 _RS23870 BWI76_RS23875: BWI76_RS23875 - glycine cleavage system protein H, at 4,983,489 to 4,983,878 _RS23875 BWI76_RS23880: BWI76_RS23880 - Aminomethyltransferase (EC 2.1.2.10) (from data), at 4,983,901 to 4,984,995 _RS23880 Position (kb) 4980 4981 4982 4983 4984Strain fitness (log2 ratio) -2 -1 0 1at 4979.524 kb on + strandat 4979.556 kb on - strandat 4979.580 kb on + strandat 4979.590 kb on + strandat 4979.598 kb on - strandat 4979.606 kb on - strandat 4979.844 kb on + strand, within BWI76_RS23865at 4980.004 kb on + strand, within BWI76_RS23865at 4980.012 kb on - strand, within BWI76_RS23865at 4980.038 kb on + strand, within BWI76_RS23865at 4980.154 kb on + strand, within BWI76_RS23865at 4980.162 kb on - strand, within BWI76_RS23865at 4980.213 kb on + strand, within BWI76_RS23865at 4980.309 kb on - strand, within BWI76_RS23865at 4980.309 kb on - strand, within BWI76_RS23865at 4980.339 kb on - strandat 4980.564 kb on + strandat 4980.778 kb on - strand, within BWI76_RS23870at 4981.058 kb on + strand, within BWI76_RS23870at 4981.219 kb on + strand, within BWI76_RS23870at 4981.236 kb on - strand, within BWI76_RS23870at 4981.273 kb on + strand, within BWI76_RS23870at 4981.679 kb on + strand, within BWI76_RS23870at 4981.905 kb on + strand, within BWI76_RS23870at 4981.989 kb on - strand, within BWI76_RS23870at 4982.075 kb on + strand, within BWI76_RS23870at 4982.133 kb on - strand, within BWI76_RS23870at 4982.162 kb on + strand, within BWI76_RS23870at 4982.182 kb on - strand, within BWI76_RS23870at 4982.185 kb on - strand, within BWI76_RS23870at 4982.395 kb on + strand, within BWI76_RS23870at 4982.405 kb on + strand, within BWI76_RS23870at 4982.408 kb on - strand, within BWI76_RS23870at 4982.410 kb on + strand, within BWI76_RS23870at 4982.529 kb on + strand, within BWI76_RS23870at 4982.685 kb on - strand, within BWI76_RS23870at 4983.048 kb on - strand, within BWI76_RS23870at 4983.084 kb on - strandat 4983.250 kb on + strandat 4983.269 kb on + strandat 4983.334 kb on - strandat 4983.461 kb on + strandat 4983.608 kb on + strand, within BWI76_RS23875at 4983.608 kb on + strand, within BWI76_RS23875at 4983.616 kb on - strandat 4983.616 kb on - strand, within BWI76_RS23875at 4983.719 kb on + strand, within BWI76_RS23875at 4983.737 kb on + strand, within BWI76_RS23875at 4984.179 kb on + strand, within BWI76_RS23880at 4984.294 kb on + strand, within BWI76_RS23880

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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4,979,524 + +0.2
4,979,556 - +0.1
4,979,580 + -0.1
4,979,590 + +0.2
4,979,598 - -0.3
4,979,606 - +0.9
4,979,844 + BWI76_RS23865 0.24 -0.1
4,980,004 + BWI76_RS23865 0.45 -2.7
4,980,012 - BWI76_RS23865 0.46 -0.9
4,980,038 + BWI76_RS23865 0.50 +0.2
4,980,154 + BWI76_RS23865 0.65 -0.7
4,980,162 - BWI76_RS23865 0.67 +1.1
4,980,213 + BWI76_RS23865 0.73 +0.3
4,980,309 - BWI76_RS23865 0.86 -1.7
4,980,309 - BWI76_RS23865 0.86 +0.3
4,980,339 - +0.4
4,980,564 + -0.5
4,980,778 - BWI76_RS23870 0.11 -0.7
4,981,058 + BWI76_RS23870 0.20 -0.8
4,981,219 + BWI76_RS23870 0.26 +0.3
4,981,236 - BWI76_RS23870 0.27 -0.4
4,981,273 + BWI76_RS23870 0.28 +0.0
4,981,679 + BWI76_RS23870 0.42 -0.0
4,981,905 + BWI76_RS23870 0.50 -0.6
4,981,989 - BWI76_RS23870 0.53 -0.0
4,982,075 + BWI76_RS23870 0.56 +1.0
4,982,133 - BWI76_RS23870 0.58 -0.4
4,982,162 + BWI76_RS23870 0.59 -0.5
4,982,182 - BWI76_RS23870 0.60 -0.4
4,982,185 - BWI76_RS23870 0.60 +0.3
4,982,395 + BWI76_RS23870 0.67 -0.4
4,982,405 + BWI76_RS23870 0.67 -0.4
4,982,408 - BWI76_RS23870 0.67 -0.3
4,982,410 + BWI76_RS23870 0.67 -0.1
4,982,529 + BWI76_RS23870 0.72 -0.8
4,982,685 - BWI76_RS23870 0.77 -0.3
4,983,048 - BWI76_RS23870 0.90 -0.6
4,983,084 - -0.2
4,983,250 + -0.0
4,983,269 + -0.4
4,983,334 - +0.2
4,983,461 + -0.2
4,983,608 + BWI76_RS23875 0.31 +0.0
4,983,608 + BWI76_RS23875 0.31 -0.3
4,983,616 - -0.1
4,983,616 - BWI76_RS23875 0.33 -0.1
4,983,719 + BWI76_RS23875 0.59 -0.4
4,983,737 + BWI76_RS23875 0.64 -0.3
4,984,179 + BWI76_RS23880 0.25 -0.6
4,984,294 + BWI76_RS23880 0.36 -0.8

Or see this region's nucleotide sequence