Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21225

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21215 and BWI76_RS21220 are separated by 73 nucleotidesBWI76_RS21220 and BWI76_RS21225 are separated by 55 nucleotidesBWI76_RS21225 and BWI76_RS21230 overlap by 14 nucleotidesBWI76_RS21230 and BWI76_RS21235 overlap by 15 nucleotidesBWI76_RS21235 and BWI76_RS21240 overlap by 56 nucleotides BWI76_RS21215: BWI76_RS21215 - NAGC-like transcriptional regulator, at 4,443,874 to 4,445,070 _RS21215 BWI76_RS21220: BWI76_RS21220 - membrane protein, at 4,445,144 to 4,445,566 _RS21220 BWI76_RS21225: BWI76_RS21225 - serine hydroxymethyltransferase, at 4,445,622 to 4,446,875 _RS21225 BWI76_RS21230: BWI76_RS21230 - hypothetical protein, at 4,446,862 to 4,447,091 _RS21230 BWI76_RS21235: BWI76_RS21235 - hypothetical protein, at 4,447,077 to 4,447,256 _RS21235 BWI76_RS21240: BWI76_RS21240 - flavohemoprotein, at 4,447,201 to 4,448,391 _RS21240 Position (kb) 4445 4446 4447Strain fitness (log2 ratio) -1 0 1at 4444.755 kb on + strand, within BWI76_RS21215at 4444.887 kb on - strand, within BWI76_RS21215at 4444.934 kb on + strand, within BWI76_RS21215at 4445.027 kb on - strandat 4445.165 kb on + strandat 4445.168 kb on - strandat 4445.270 kb on + strand, within BWI76_RS21220at 4445.283 kb on - strand, within BWI76_RS21220at 4445.423 kb on + strand, within BWI76_RS21220at 4445.519 kb on - strand, within BWI76_RS21220at 4445.573 kb on - strandat 4446.973 kb on - strand, within BWI76_RS21230at 4447.198 kb on + strand, within BWI76_RS21235at 4447.259 kb on - strandat 4447.357 kb on - strand, within BWI76_RS21240at 4447.482 kb on + strand, within BWI76_RS21240at 4447.482 kb on + strand, within BWI76_RS21240at 4447.584 kb on - strand, within BWI76_RS21240at 4447.854 kb on - strand, within BWI76_RS21240

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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4,444,755 + BWI76_RS21215 0.74 -0.1
4,444,887 - BWI76_RS21215 0.85 +0.1
4,444,934 + BWI76_RS21215 0.89 +0.1
4,445,027 - -0.3
4,445,165 + +0.1
4,445,168 - -0.2
4,445,270 + BWI76_RS21220 0.30 -0.1
4,445,283 - BWI76_RS21220 0.33 +0.2
4,445,423 + BWI76_RS21220 0.66 +0.2
4,445,519 - BWI76_RS21220 0.89 +0.2
4,445,573 - -0.1
4,446,973 - BWI76_RS21230 0.48 -0.7
4,447,198 + BWI76_RS21235 0.67 -0.4
4,447,259 - +1.5
4,447,357 - BWI76_RS21240 0.13 -0.1
4,447,482 + BWI76_RS21240 0.24 -0.0
4,447,482 + BWI76_RS21240 0.24 +0.3
4,447,584 - BWI76_RS21240 0.32 -0.4
4,447,854 - BWI76_RS21240 0.55 +0.3

Or see this region's nucleotide sequence