Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20960 and BWI76_RS20965 are separated by 265 nucleotides BWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotides BWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotides
BWI76_RS20960: BWI76_RS20960 - ROK family protein, at 4,381,211 to 4,382,104
_RS20960
BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407
_RS20965
BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045
_RS20970
BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447
_RS20975
Position (kb) 4382
4383
4384 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4381.419 kb on + strand, within BWI76_RS20960 at 4381.427 kb on - strand, within BWI76_RS20960 at 4381.475 kb on - strand, within BWI76_RS20960 at 4381.839 kb on - strand, within BWI76_RS20960 at 4381.839 kb on - strand, within BWI76_RS20960 at 4382.355 kb on + strand at 4382.628 kb on - strand, within BWI76_RS20965 at 4382.639 kb on - strand, within BWI76_RS20965 at 4383.163 kb on - strand, within BWI76_RS20965 at 4383.337 kb on - strand at 4383.346 kb on - strand at 4383.379 kb on + strand at 4383.431 kb on + strand at 4383.859 kb on + strand, within BWI76_RS20970 at 4384.255 kb on + strand, within BWI76_RS20975
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,381,419 + BWI76_RS20960 0.23 +0.5 4,381,427 - BWI76_RS20960 0.24 -0.1 4,381,475 - BWI76_RS20960 0.30 +0.8 4,381,839 - BWI76_RS20960 0.70 +0.1 4,381,839 - BWI76_RS20960 0.70 -0.5 4,382,355 + -1.4 4,382,628 - BWI76_RS20965 0.25 -5.0 4,382,639 - BWI76_RS20965 0.26 -0.8 4,383,163 - BWI76_RS20965 0.76 -2.4 4,383,337 - -1.7 4,383,346 - -2.4 4,383,379 + -0.0 4,383,431 + -0.4 4,383,859 + BWI76_RS20970 0.71 -0.5 4,384,255 + BWI76_RS20975 0.12 -0.2
Or see this region's nucleotide sequence