Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20350 and BWI76_RS20355 are separated by 33 nucleotides BWI76_RS20355 and BWI76_RS20360 are separated by 199 nucleotides BWI76_RS20360 and BWI76_RS20365 overlap by 11 nucleotides
BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356
_RS20350
BWI76_RS20355: BWI76_RS20355 - colicin V production protein, at 4,261,390 to 4,261,878
_RS20355
BWI76_RS20360: BWI76_RS20360 - cell division protein DedD, at 4,262,078 to 4,262,767
_RS20360
BWI76_RS20365: BWI76_RS20365 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 4,262,757 to 4,264,025
_RS20365
Position (kb) 4262
4263 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4261.323 kb on + strand at 4261.470 kb on + strand, within BWI76_RS20355 at 4261.551 kb on - strand, within BWI76_RS20355 at 4261.570 kb on + strand, within BWI76_RS20355 at 4262.158 kb on + strand, within BWI76_RS20360
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,261,323 + -1.9 4,261,470 + BWI76_RS20355 0.16 -1.8 4,261,551 - BWI76_RS20355 0.33 -2.0 4,261,570 + BWI76_RS20355 0.37 -3.5 4,262,158 + BWI76_RS20360 0.12 -0.8
Or see this region's nucleotide sequence