Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20165 and BWI76_RS20170 are separated by 32 nucleotides BWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotides BWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotides
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467
_RS20170
BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964
_RS20175
BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763
_RS20180
Position (kb)
4227
4228
4229 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4226.418 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.908 kb on - strand at 4227.802 kb on - strand, within BWI76_RS20170 at 4227.826 kb on + strand, within BWI76_RS20170 at 4227.944 kb on + strand, within BWI76_RS20170 at 4228.008 kb on - strand, within BWI76_RS20170 at 4228.295 kb on - strand, within BWI76_RS20170 at 4228.438 kb on + strand at 4228.521 kb on - strand, within BWI76_RS20175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,226,418 - BWI76_RS20165 0.75 -2.2 4,226,753 - BWI76_RS20165 0.87 -2.1 4,226,753 - BWI76_RS20165 0.87 +0.0 4,226,908 - -2.0 4,227,802 - BWI76_RS20170 0.50 -1.0 4,227,826 + BWI76_RS20170 0.52 -0.8 4,227,944 + BWI76_RS20170 0.61 -1.8 4,228,008 - BWI76_RS20170 0.66 -2.0 4,228,295 - BWI76_RS20170 0.87 -1.8 4,228,438 + -0.8 4,228,521 - BWI76_RS20175 0.11 -2.8
Or see this region's nucleotide sequence