Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotides BWI76_RS18085 and BWI76_RS18090 are separated by 135 nucleotides BWI76_RS18090 and BWI76_RS18095 are separated by 45 nucleotides BWI76_RS18095 and BWI76_RS18100 are separated by 37 nucleotides
BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309
_RS18080
BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816
_RS18085
BWI76_RS18090: BWI76_RS18090 - phosphoribosylglycinamide formyltransferase 2, at 3,757,952 to 3,759,130
_RS18090
BWI76_RS18095: BWI76_RS18095 - ketohydroxyglutarate aldolase, at 3,759,176 to 3,759,817
_RS18095
BWI76_RS18100: BWI76_RS18100 - phosphogluconate dehydratase, at 3,759,855 to 3,761,666
_RS18100
Position (kb)
3757
3758
3759
3760 Strain fitness (log2 ratio)
-1
0
1 at 3757.080 kb on + strand, within BWI76_RS18080 at 3757.534 kb on - strand, within BWI76_RS18085 at 3757.869 kb on + strand at 3757.992 kb on + strand at 3758.028 kb on - strand at 3758.214 kb on + strand, within BWI76_RS18090 at 3758.441 kb on + strand, within BWI76_RS18090 at 3759.581 kb on - strand, within BWI76_RS18095 at 3759.884 kb on - strand at 3759.884 kb on - strand at 3759.886 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,757,080 + BWI76_RS18080 0.66 +0.1 3,757,534 - BWI76_RS18085 0.22 -0.3 3,757,869 + -0.1 3,757,992 + -0.4 3,758,028 - -1.0 3,758,214 + BWI76_RS18090 0.22 -0.2 3,758,441 + BWI76_RS18090 0.41 -0.3 3,759,581 - BWI76_RS18095 0.63 -0.3 3,759,884 - -0.6 3,759,884 - +0.2 3,759,886 + -0.1
Or see this region's nucleotide sequence