Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18090

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotidesBWI76_RS18085 and BWI76_RS18090 are separated by 135 nucleotidesBWI76_RS18090 and BWI76_RS18095 are separated by 45 nucleotidesBWI76_RS18095 and BWI76_RS18100 are separated by 37 nucleotides BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309 _RS18080 BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816 _RS18085 BWI76_RS18090: BWI76_RS18090 - phosphoribosylglycinamide formyltransferase 2, at 3,757,952 to 3,759,130 _RS18090 BWI76_RS18095: BWI76_RS18095 - ketohydroxyglutarate aldolase, at 3,759,176 to 3,759,817 _RS18095 BWI76_RS18100: BWI76_RS18100 - phosphogluconate dehydratase, at 3,759,855 to 3,761,666 _RS18100 Position (kb) 3757 3758 3759 3760Strain fitness (log2 ratio) -1 0 1at 3757.080 kb on + strand, within BWI76_RS18080at 3757.534 kb on - strand, within BWI76_RS18085at 3757.869 kb on + strandat 3757.992 kb on + strandat 3758.028 kb on - strandat 3758.214 kb on + strand, within BWI76_RS18090at 3758.441 kb on + strand, within BWI76_RS18090at 3759.581 kb on - strand, within BWI76_RS18095at 3759.884 kb on - strandat 3759.884 kb on - strandat 3759.886 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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3,757,080 + BWI76_RS18080 0.66 +0.1
3,757,534 - BWI76_RS18085 0.22 -0.3
3,757,869 + -0.1
3,757,992 + -0.4
3,758,028 - -1.0
3,758,214 + BWI76_RS18090 0.22 -0.2
3,758,441 + BWI76_RS18090 0.41 -0.3
3,759,581 - BWI76_RS18095 0.63 -0.3
3,759,884 - -0.6
3,759,884 - +0.2
3,759,886 + -0.1

Or see this region's nucleotide sequence