Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16260 and BWI76_RS16265 are separated by 74 nucleotides BWI76_RS16265 and BWI76_RS16270 overlap by 11 nucleotides BWI76_RS16270 and BWI76_RS16275 are separated by 142 nucleotides
BWI76_RS16260: BWI76_RS16260 - two-component sensor histidine kinase, at 3,372,842 to 3,374,143
_RS16260
BWI76_RS16265: BWI76_RS16265 - DNA replication terminus site-binding protein, at 3,374,218 to 3,375,150
_RS16265
BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540
_RS16270
BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329
_RS16275
Position (kb)
3375
3376
3377 Strain fitness (log2 ratio)
-1
0
1 at 3374.965 kb on + strand, within BWI76_RS16265 at 3375.238 kb on + strand at 3375.706 kb on + strand, within BWI76_RS16270 at 3376.030 kb on + strand, within BWI76_RS16270 at 3376.146 kb on + strand, within BWI76_RS16270 at 3376.166 kb on - strand, within BWI76_RS16270 at 3376.270 kb on + strand, within BWI76_RS16270 at 3376.367 kb on - strand, within BWI76_RS16270 at 3376.473 kb on + strand at 3376.531 kb on + strand at 3376.909 kb on - strand, within BWI76_RS16275
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,374,965 + BWI76_RS16265 0.80 +0.3 3,375,238 + -0.3 3,375,706 + BWI76_RS16270 0.40 +0.6 3,376,030 + BWI76_RS16270 0.64 +0.1 3,376,146 + BWI76_RS16270 0.72 +0.1 3,376,166 - BWI76_RS16270 0.73 +0.0 3,376,270 + BWI76_RS16270 0.81 -0.4 3,376,367 - BWI76_RS16270 0.88 -0.0 3,376,473 + +0.9 3,376,531 + -0.4 3,376,909 - BWI76_RS16275 0.14 +1.2
Or see this region's nucleotide sequence