Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16225 and BWI76_RS16230 are separated by 36 nucleotides BWI76_RS16230 and BWI76_RS16235 overlap by 4 nucleotides BWI76_RS16235 and BWI76_RS16240 are separated by 990 nucleotides
BWI76_RS16225: BWI76_RS16225 - arginine:ornithine antiporter, at 3,365,468 to 3,366,850
_RS16225
BWI76_RS16230: BWI76_RS16230 - dihydromonapterin reductase, at 3,366,887 to 3,367,612
_RS16230
BWI76_RS16235: BWI76_RS16235 - membrane protein, at 3,367,609 to 3,367,944
_RS16235
BWI76_RS16240: BWI76_RS16240 - anti-adapter protein IraM, at 3,368,935 to 3,369,282
_RS16240
Position (kb)
3367
3368 Strain fitness (log2 ratio)
-2
-1
0
1 at 3367.111 kb on + strand, within BWI76_RS16230 at 3367.342 kb on - strand, within BWI76_RS16230 at 3367.527 kb on + strand at 3367.557 kb on - strand at 3367.647 kb on + strand, within BWI76_RS16235 at 3367.654 kb on + strand, within BWI76_RS16235 at 3367.816 kb on - strand, within BWI76_RS16235 at 3368.773 kb on - strand at 3368.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,367,111 + BWI76_RS16230 0.31 -0.8 3,367,342 - BWI76_RS16230 0.63 -0.3 3,367,527 + +0.6 3,367,557 - -1.3 3,367,647 + BWI76_RS16235 0.11 -1.9 3,367,654 + BWI76_RS16235 0.13 -1.6 3,367,816 - BWI76_RS16235 0.62 -2.5 3,368,773 - -1.2 3,368,900 - -0.1
Or see this region's nucleotide sequence