Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15220 and BWI76_RS15225 are separated by 12 nucleotides BWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotides BWI76_RS15230 and BWI76_RS15235 overlap by 8 nucleotides
BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038
_RS15220
BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812
_RS15225
BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800
_RS15230
BWI76_RS15235: BWI76_RS15235 - hypothetical protein, at 3,192,793 to 3,194,031
_RS15235
Position (kb)
3191
3192 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3190.233 kb on + strand, within BWI76_RS15220 at 3190.611 kb on + strand, within BWI76_RS15220 at 3190.619 kb on - strand, within BWI76_RS15220 at 3191.144 kb on + strand, within BWI76_RS15225 at 3191.152 kb on - strand, within BWI76_RS15225 at 3191.276 kb on - strand, within BWI76_RS15225 at 3191.685 kb on + strand, within BWI76_RS15225 at 3191.693 kb on - strand, within BWI76_RS15225 at 3191.848 kb on - strand at 3191.851 kb on - strand at 3192.218 kb on + strand, within BWI76_RS15230 at 3192.243 kb on - strand, within BWI76_RS15230 at 3192.280 kb on + strand, within BWI76_RS15230 at 3192.316 kb on + strand, within BWI76_RS15230 at 3192.592 kb on + strand, within BWI76_RS15230 at 3192.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,190,233 + BWI76_RS15220 0.15 -0.2 3,190,611 + BWI76_RS15220 0.55 +0.1 3,190,619 - BWI76_RS15220 0.56 -0.1 3,191,144 + BWI76_RS15225 0.12 -1.2 3,191,152 - BWI76_RS15225 0.13 -2.7 3,191,276 - BWI76_RS15225 0.30 -0.9 3,191,685 + BWI76_RS15225 0.83 -2.7 3,191,693 - BWI76_RS15225 0.84 -3.0 3,191,848 - +0.0 3,191,851 - +0.8 3,192,218 + BWI76_RS15230 0.41 +0.0 3,192,243 - BWI76_RS15230 0.43 -0.1 3,192,280 + BWI76_RS15230 0.47 +0.2 3,192,316 + BWI76_RS15230 0.51 -2.0 3,192,592 + BWI76_RS15230 0.79 -0.4 3,192,728 - +0.2
Or see this region's nucleotide sequence